Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS11060

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS11050 and RS_RS11055 are separated by 40 nucleotidesRS_RS11055 and RS_RS11060 are separated by 5 nucleotidesRS_RS11060 and RS_RS11065 are separated by 104 nucleotides RS_RS11050: RS_RS11050 - glycosyltransferase family 2 protein, at 2,383,869 to 2,384,639 _RS11050 RS_RS11055: RS_RS11055 - glycosyltransferase family 2 protein, at 2,384,680 to 2,385,600 _RS11055 RS_RS11060: RS_RS11060 - O-antigen ligase family protein, at 2,385,606 to 2,386,913 _RS11060 RS_RS11065: RS_RS11065 - DNA polymerase III subunit alpha, at 2,387,018 to 2,390,539 _RS11065 Position (kb) 2385 2386 2387Strain fitness (log2 ratio) -2 -1 0 1at 2384.780 kb on + strand, within RS_RS11055at 2384.781 kb on - strand, within RS_RS11055at 2384.969 kb on + strand, within RS_RS11055at 2384.970 kb on - strand, within RS_RS11055at 2385.168 kb on - strand, within RS_RS11055at 2385.168 kb on - strand, within RS_RS11055at 2385.438 kb on + strand, within RS_RS11055at 2385.439 kb on - strand, within RS_RS11055at 2385.439 kb on - strand, within RS_RS11055at 2385.591 kb on + strandat 2385.695 kb on - strandat 2385.896 kb on - strand, within RS_RS11060at 2386.051 kb on + strand, within RS_RS11060at 2386.159 kb on + strand, within RS_RS11060at 2386.221 kb on - strand, within RS_RS11060at 2386.837 kb on + strandat 2386.868 kb on - strandat 2386.919 kb on + strandat 2386.920 kb on - strandat 2386.920 kb on - strandat 2386.988 kb on + strandat 2386.988 kb on + strandat 2386.988 kb on + strandat 2386.988 kb on + strandat 2386.988 kb on + strandat 2386.989 kb on - strandat 2386.989 kb on - strandat 2386.989 kb on - strandat 2386.989 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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2,384,780 + RS_RS11055 0.11 -1.8
2,384,781 - RS_RS11055 0.11 -1.2
2,384,969 + RS_RS11055 0.31 -0.4
2,384,970 - RS_RS11055 0.31 -2.4
2,385,168 - RS_RS11055 0.53 -2.5
2,385,168 - RS_RS11055 0.53 -1.9
2,385,438 + RS_RS11055 0.82 +1.1
2,385,439 - RS_RS11055 0.82 -0.9
2,385,439 - RS_RS11055 0.82 -2.2
2,385,591 + -1.7
2,385,695 - -1.2
2,385,896 - RS_RS11060 0.22 -0.8
2,386,051 + RS_RS11060 0.34 -1.0
2,386,159 + RS_RS11060 0.42 -1.2
2,386,221 - RS_RS11060 0.47 -2.3
2,386,837 + -0.1
2,386,868 - +0.3
2,386,919 + -2.1
2,386,920 - -1.7
2,386,920 - +0.1
2,386,988 + -0.4
2,386,988 + +0.0
2,386,988 + +0.2
2,386,988 + -0.1
2,386,988 + +1.4
2,386,989 - -0.2
2,386,989 - -0.1
2,386,989 - -0.2
2,386,989 - -0.2

Or see this region's nucleotide sequence