Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS10215

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS10205 and RS_RS10210 are separated by 30 nucleotidesRS_RS10210 and RS_RS10215 are separated by 42 nucleotidesRS_RS10215 and RS_RS10220 are separated by 99 nucleotidesRS_RS10220 and RS_RS10225 are separated by 13 nucleotides RS_RS10205: RS_RS10205 - urease accessory protein UreJ, at 2,208,122 to 2,208,739 _RS10205 RS_RS10210: RS_RS10210 - urease subunit gamma, at 2,208,770 to 2,209,072 _RS10210 RS_RS10215: RS_RS10215 - urease accessory protein, at 2,209,115 to 2,210,002 _RS10215 RS_RS10220: RS_RS10220 - urea ABC transporter ATP-binding subunit UrtE, at 2,210,102 to 2,210,794 _RS10220 RS_RS10225: RS_RS10225 - urea ABC transporter ATP-binding protein UrtD, at 2,210,808 to 2,211,671 _RS10225 Position (kb) 2209 2210 2211Strain fitness (log2 ratio) -1 0 1at 2208.901 kb on - strand, within RS_RS10210at 2208.901 kb on - strand, within RS_RS10210at 2209.084 kb on + strandat 2209.711 kb on - strand, within RS_RS10215at 2210.451 kb on + strand, within RS_RS10220at 2210.451 kb on + strand, within RS_RS10220at 2210.556 kb on + strand, within RS_RS10220at 2210.556 kb on + strand, within RS_RS10220at 2210.556 kb on + strand, within RS_RS10220at 2210.766 kb on + strandat 2210.767 kb on - strandat 2210.821 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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2,208,901 - RS_RS10210 0.43 -0.4
2,208,901 - RS_RS10210 0.43 -0.8
2,209,084 + +0.1
2,209,711 - RS_RS10215 0.67 +0.1
2,210,451 + RS_RS10220 0.50 -0.0
2,210,451 + RS_RS10220 0.50 +1.1
2,210,556 + RS_RS10220 0.66 +0.0
2,210,556 + RS_RS10220 0.66 -0.2
2,210,556 + RS_RS10220 0.66 -0.4
2,210,766 + +0.4
2,210,767 - -0.1
2,210,821 + -0.3

Or see this region's nucleotide sequence