Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS05080

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS05070 and RS_RS05075 are separated by 43 nucleotidesRS_RS05075 and RS_RS05080 are separated by 41 nucleotidesRS_RS05080 and RS_RS05085 are separated by 208 nucleotides RS_RS05070: RS_RS05070 - molecular chaperone HscA, at 1,075,903 to 1,077,768 _RS05070 RS_RS05075: RS_RS05075 - ISC system 2Fe-2S type ferredoxin, at 1,077,812 to 1,078,150 _RS05075 RS_RS05080: RS_RS05080 - Fe-S assembly protein IscX, at 1,078,192 to 1,078,389 _RS05080 RS_RS05085: RS_RS05085 - glycine zipper 2TM domain-containing protein, at 1,078,598 to 1,079,350 _RS05085 Position (kb) 1078 1079Strain fitness (log2 ratio) -1 0 1at 1078.166 kb on + strandat 1078.232 kb on - strand, within RS_RS05080at 1078.435 kb on - strandat 1078.542 kb on + strandat 1078.542 kb on + strandat 1078.542 kb on + strandat 1078.542 kb on + strandat 1078.668 kb on + strandat 1078.668 kb on + strandat 1078.669 kb on - strandat 1078.669 kb on - strandat 1078.735 kb on - strand, within RS_RS05085at 1078.735 kb on - strand, within RS_RS05085at 1078.735 kb on - strand, within RS_RS05085at 1078.880 kb on - strand, within RS_RS05085at 1078.960 kb on - strand, within RS_RS05085at 1078.999 kb on - strand, within RS_RS05085

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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1,078,166 + -0.1
1,078,232 - RS_RS05080 0.20 -0.1
1,078,435 - -0.2
1,078,542 + +0.3
1,078,542 + +0.2
1,078,542 + +0.0
1,078,542 + +0.2
1,078,668 + +0.1
1,078,668 + -0.3
1,078,669 - -0.0
1,078,669 - +0.5
1,078,735 - RS_RS05085 0.18 -0.8
1,078,735 - RS_RS05085 0.18 +0.7
1,078,735 - RS_RS05085 0.18 -0.5
1,078,880 - RS_RS05085 0.37 -0.6
1,078,960 - RS_RS05085 0.48 +0.6
1,078,999 - RS_RS05085 0.53 -0.7

Or see this region's nucleotide sequence