Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS00950 and RS_RS00955 are separated by 103 nucleotides RS_RS00955 and RS_RS00960 are separated by 239 nucleotides RS_RS00960 and RS_RS00965 are separated by 45 nucleotides
RS_RS00950: RS_RS00950 - DNA-binding response regulator, at 213,632 to 214,279
_RS00950
RS_RS00955: RS_RS00955 - hypothetical protein, at 214,383 to 214,877
_RS00955
RS_RS00960: RS_RS00960 - alcohol dehydrogenase, at 215,117 to 216,154
_RS00960
RS_RS00965: RS_RS00965 - ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent, at 216,200 to 218,017
_RS00965
Position (kb) 215
216
217 Strain fitness (log2 ratio) -1
0
1 at 214.171 kb on + strand, within RS_RS00950 at 214.171 kb on + strand, within RS_RS00950 at 214.178 kb on + strand, within RS_RS00950 at 214.178 kb on + strand, within RS_RS00950 at 214.213 kb on + strand, within RS_RS00950 at 214.236 kb on - strand at 214.351 kb on + strand at 214.352 kb on - strand at 214.352 kb on - strand at 214.381 kb on + strand at 214.589 kb on - strand, within RS_RS00955 at 214.681 kb on - strand, within RS_RS00955 at 214.707 kb on - strand, within RS_RS00955 at 214.944 kb on - strand at 214.971 kb on - strand at 215.054 kb on - strand at 215.062 kb on - strand at 215.082 kb on + strand at 215.082 kb on + strand at 215.083 kb on - strand at 215.245 kb on + strand, within RS_RS00960 at 215.245 kb on + strand, within RS_RS00960 at 215.245 kb on + strand, within RS_RS00960 at 215.246 kb on - strand, within RS_RS00960 at 215.374 kb on + strand, within RS_RS00960 at 215.375 kb on - strand, within RS_RS00960 at 215.417 kb on + strand, within RS_RS00960 at 215.417 kb on + strand, within RS_RS00960 at 215.480 kb on + strand, within RS_RS00960 at 215.481 kb on - strand, within RS_RS00960 at 215.513 kb on + strand, within RS_RS00960 at 215.520 kb on + strand, within RS_RS00960 at 215.563 kb on + strand, within RS_RS00960 at 215.564 kb on - strand, within RS_RS00960 at 215.925 kb on + strand, within RS_RS00960 at 216.153 kb on - strand at 216.153 kb on - strand at 216.157 kb on + strand at 216.269 kb on + strand at 216.270 kb on - strand at 216.377 kb on + strand at 216.378 kb on - strand at 216.730 kb on + strand, within RS_RS00965 at 216.731 kb on - strand, within RS_RS00965 at 216.731 kb on - strand, within RS_RS00965 at 216.798 kb on + strand, within RS_RS00965 at 216.877 kb on + strand, within RS_RS00965 at 216.932 kb on + strand, within RS_RS00965 at 216.933 kb on - strand, within RS_RS00965 at 216.933 kb on - strand, within RS_RS00965
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 214,171 + RS_RS00950 0.83 -1.6 214,171 + RS_RS00950 0.83 -0.4 214,178 + RS_RS00950 0.84 +0.1 214,178 + RS_RS00950 0.84 +1.4 214,213 + RS_RS00950 0.90 -0.2 214,236 - -0.0 214,351 + -0.1 214,352 - -0.1 214,352 - +1.4 214,381 + -0.1 214,589 - RS_RS00955 0.42 +0.8 214,681 - RS_RS00955 0.60 -0.8 214,707 - RS_RS00955 0.65 -1.1 214,944 - +0.4 214,971 - -0.1 215,054 - +0.3 215,062 - -0.8 215,082 + -0.1 215,082 + +0.1 215,083 - +0.5 215,245 + RS_RS00960 0.12 +0.3 215,245 + RS_RS00960 0.12 -0.8 215,245 + RS_RS00960 0.12 -0.8 215,246 - RS_RS00960 0.12 -0.2 215,374 + RS_RS00960 0.25 +0.2 215,375 - RS_RS00960 0.25 +0.2 215,417 + RS_RS00960 0.29 +0.2 215,417 + RS_RS00960 0.29 -0.4 215,480 + RS_RS00960 0.35 +0.6 215,481 - RS_RS00960 0.35 +0.3 215,513 + RS_RS00960 0.38 +0.0 215,520 + RS_RS00960 0.39 -0.4 215,563 + RS_RS00960 0.43 -0.2 215,564 - RS_RS00960 0.43 -0.1 215,925 + RS_RS00960 0.78 -0.6 216,153 - +0.1 216,153 - -0.3 216,157 + +0.3 216,269 + -0.3 216,270 - -0.7 216,377 + -0.0 216,378 - +0.8 216,730 + RS_RS00965 0.29 -0.1 216,731 - RS_RS00965 0.29 -0.1 216,731 - RS_RS00965 0.29 +1.4 216,798 + RS_RS00965 0.33 -0.3 216,877 + RS_RS00965 0.37 +0.1 216,932 + RS_RS00965 0.40 +0.5 216,933 - RS_RS00965 0.40 -0.3 216,933 - RS_RS00965 0.40 +0.6
Or see this region's nucleotide sequence