Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS00855 and RS_RS00860 are separated by 198 nucleotides RS_RS00860 and RS_RS00865 are separated by 160 nucleotides
RS_RS00855: RS_RS00855 - membrane protein, at 194,451 to 195,875
_RS00855
RS_RS00860: RS_RS00860 - 2-dehydropantoate 2-reductase, at 196,074 to 197,015
_RS00860
RS_RS00865: RS_RS00865 - tRNA 2-thiocytidine(32) synthetase TtcA, at 197,176 to 198,114
_RS00865
Position (kb) 196
197
198 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 195.166 kb on + strand, within RS_RS00855 at 195.167 kb on - strand, within RS_RS00855 at 195.409 kb on + strand, within RS_RS00855 at 195.409 kb on + strand, within RS_RS00855 at 195.410 kb on - strand, within RS_RS00855 at 195.410 kb on - strand, within RS_RS00855 at 195.677 kb on - strand, within RS_RS00855 at 195.689 kb on + strand, within RS_RS00855 at 195.769 kb on + strand at 195.770 kb on - strand at 195.770 kb on - strand at 195.797 kb on - strand at 195.834 kb on + strand at 195.834 kb on + strand at 195.834 kb on + strand at 195.834 kb on + strand at 195.835 kb on - strand at 195.835 kb on - strand at 195.908 kb on + strand at 195.909 kb on - strand at 195.997 kb on + strand at 195.998 kb on - strand at 195.998 kb on - strand at 195.998 kb on - strand at 195.998 kb on - strand at 196.000 kb on + strand at 196.000 kb on + strand at 196.000 kb on + strand at 196.000 kb on + strand at 196.000 kb on + strand at 196.000 kb on + strand at 196.000 kb on + strand at 196.001 kb on - strand at 196.001 kb on - strand at 196.001 kb on - strand at 196.001 kb on - strand at 196.001 kb on - strand at 196.001 kb on - strand at 196.075 kb on + strand at 196.075 kb on + strand at 196.109 kb on - strand at 196.347 kb on + strand, within RS_RS00860 at 196.348 kb on + strand, within RS_RS00860 at 196.348 kb on + strand, within RS_RS00860 at 196.349 kb on - strand, within RS_RS00860 at 196.349 kb on - strand, within RS_RS00860 at 196.349 kb on - strand, within RS_RS00860 at 196.777 kb on + strand, within RS_RS00860 at 196.777 kb on + strand, within RS_RS00860 at 197.002 kb on - strand at 197.052 kb on - strand at 197.052 kb on - strand at 197.308 kb on + strand, within RS_RS00865 at 197.308 kb on + strand, within RS_RS00865 at 197.308 kb on + strand, within RS_RS00865 at 197.308 kb on + strand, within RS_RS00865 at 197.309 kb on - strand, within RS_RS00865 at 197.309 kb on - strand, within RS_RS00865 at 197.426 kb on - strand, within RS_RS00865 at 197.449 kb on + strand, within RS_RS00865 at 197.449 kb on + strand, within RS_RS00865 at 197.449 kb on + strand, within RS_RS00865 at 197.450 kb on - strand, within RS_RS00865 at 197.474 kb on - strand, within RS_RS00865 at 197.551 kb on + strand, within RS_RS00865 at 197.551 kb on + strand, within RS_RS00865 at 197.720 kb on - strand, within RS_RS00865 at 197.998 kb on + strand, within RS_RS00865
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 195,166 + RS_RS00855 0.50 -0.0 195,167 - RS_RS00855 0.50 +0.3 195,409 + RS_RS00855 0.67 +0.1 195,409 + RS_RS00855 0.67 +0.5 195,410 - RS_RS00855 0.67 +1.4 195,410 - RS_RS00855 0.67 +0.5 195,677 - RS_RS00855 0.86 +0.0 195,689 + RS_RS00855 0.87 +0.4 195,769 + +0.4 195,770 - -0.8 195,770 - +0.3 195,797 - +0.0 195,834 + -0.2 195,834 + -0.2 195,834 + +1.4 195,834 + -0.0 195,835 - +1.4 195,835 - -0.2 195,908 + -0.2 195,909 - -0.2 195,997 + -0.2 195,998 - +0.2 195,998 - -0.0 195,998 - +0.7 195,998 - +2.0 196,000 + -0.6 196,000 + -0.2 196,000 + -0.6 196,000 + -1.2 196,000 + +0.1 196,000 + -0.1 196,000 + +0.4 196,001 - +0.8 196,001 - -1.9 196,001 - +1.4 196,001 - -0.1 196,001 - +3.4 196,001 - -0.5 196,075 + -0.2 196,075 + -0.1 196,109 - +0.1 196,347 + RS_RS00860 0.29 +2.4 196,348 + RS_RS00860 0.29 -0.1 196,348 + RS_RS00860 0.29 -0.0 196,349 - RS_RS00860 0.29 -0.0 196,349 - RS_RS00860 0.29 -0.3 196,349 - RS_RS00860 0.29 +0.2 196,777 + RS_RS00860 0.75 -0.1 196,777 + RS_RS00860 0.75 -0.3 197,002 - -0.3 197,052 - -0.1 197,052 - -0.1 197,308 + RS_RS00865 0.14 -0.7 197,308 + RS_RS00865 0.14 -0.1 197,308 + RS_RS00865 0.14 +0.5 197,308 + RS_RS00865 0.14 -0.3 197,309 - RS_RS00865 0.14 -0.8 197,309 - RS_RS00865 0.14 +0.3 197,426 - RS_RS00865 0.27 -0.3 197,449 + RS_RS00865 0.29 +0.1 197,449 + RS_RS00865 0.29 -0.3 197,449 + RS_RS00865 0.29 -0.9 197,450 - RS_RS00865 0.29 -0.4 197,474 - RS_RS00865 0.32 -0.6 197,551 + RS_RS00865 0.40 -0.2 197,551 + RS_RS00865 0.40 +0.4 197,720 - RS_RS00865 0.58 -0.4 197,998 + RS_RS00865 0.88 +0.4
Or see this region's nucleotide sequence