Experiment: MoLS4 with L-Cysteine 1mM as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proA and nadD are separated by 25 nucleotides nadD and DVU1955 overlap by 4 nucleotides DVU1955 and DVU1956 are separated by 141 nucleotides
DVU1953: proA - gamma-glutamyl phosphate reductase (TIGR), at 2,026,227 to 2,027,486
proA
DVU1954: nadD - nicotinate (nicotinamide) nucleotide adenylyltransferase (TIGR), at 2,027,512 to 2,028,216
nadD
DVU1955: DVU1955 - TPR domain protein (TIGR), at 2,028,213 to 2,029,046
DVU1955
DVU1956: DVU1956 - heptosyltransferase family protein (TIGR), at 2,029,188 to 2,030,243
DVU1956
Position (kb)
2027
2028
2029 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2026.665 kb on - strand, within proA at 2026.766 kb on - strand, within proA at 2026.799 kb on + strand, within proA at 2026.901 kb on + strand, within proA at 2026.909 kb on - strand, within proA at 2026.923 kb on + strand, within proA at 2027.090 kb on + strand, within proA at 2027.171 kb on - strand, within proA at 2027.237 kb on + strand, within proA at 2027.385 kb on + strand at 2028.181 kb on + strand at 2028.387 kb on - strand, within DVU1955 at 2028.447 kb on + strand, within DVU1955 at 2028.561 kb on - strand, within DVU1955 at 2028.644 kb on - strand, within DVU1955 at 2028.686 kb on + strand, within DVU1955 at 2028.716 kb on + strand, within DVU1955 at 2028.833 kb on - strand, within DVU1955 at 2028.855 kb on + strand, within DVU1955 at 2028.860 kb on + strand, within DVU1955 at 2028.863 kb on - strand, within DVU1955 at 2028.863 kb on - strand, within DVU1955 at 2028.863 kb on - strand, within DVU1955 at 2028.890 kb on + strand, within DVU1955 at 2028.898 kb on - strand, within DVU1955 at 2028.898 kb on - strand, within DVU1955 at 2028.898 kb on - strand, within DVU1955 at 2028.902 kb on + strand, within DVU1955 at 2028.922 kb on + strand, within DVU1955 at 2029.173 kb on + strand at 2029.181 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Cysteine 1mM as (N) remove 2,026,665 - proA DVU1953 0.35 -2.6 2,026,766 - proA DVU1953 0.43 -3.0 2,026,799 + proA DVU1953 0.45 -0.3 2,026,901 + proA DVU1953 0.53 -5.4 2,026,909 - proA DVU1953 0.54 +0.3 2,026,923 + proA DVU1953 0.55 -2.3 2,027,090 + proA DVU1953 0.68 -3.5 2,027,171 - proA DVU1953 0.75 -2.6 2,027,237 + proA DVU1953 0.80 -4.8 2,027,385 + -0.3 2,028,181 + -0.7 2,028,387 - DVU1955 0.21 +0.5 2,028,447 + DVU1955 0.28 -1.1 2,028,561 - DVU1955 0.42 -0.2 2,028,644 - DVU1955 0.52 +0.0 2,028,686 + DVU1955 0.57 -0.4 2,028,716 + DVU1955 0.60 -0.4 2,028,833 - DVU1955 0.74 -1.0 2,028,855 + DVU1955 0.77 -1.9 2,028,860 + DVU1955 0.78 +0.3 2,028,863 - DVU1955 0.78 +0.3 2,028,863 - DVU1955 0.78 +0.7 2,028,863 - DVU1955 0.78 +1.0 2,028,890 + DVU1955 0.81 +0.0 2,028,898 - DVU1955 0.82 +0.1 2,028,898 - DVU1955 0.82 -0.3 2,028,898 - DVU1955 0.82 -0.4 2,028,902 + DVU1955 0.83 +0.7 2,028,922 + DVU1955 0.85 +0.0 2,029,173 + -0.9 2,029,181 - -2.6
Or see this region's nucleotide sequence