Experiment: Lactate-Sulfate (60-50mM) with 0.1% Yeast Extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt htrA and rpsA are separated by 160 nucleotides rpsA and ppiC are separated by 153 nucleotides ppiC and hspC are separated by 72 nucleotides hspC and DVU1472 overlap by 4 nucleotides
DVU1468: htrA - peptidase/PDZ domain protein (TIGR), at 1,548,081 to 1,549,529
htrA
DVU1469: rpsA - ribosomal protein S1, putative (TIGR), at 1,549,690 to 1,551,153
rpsA
DVU1470: ppiC - peptidyl-prolyl cis-trans isomerase C (TIGR), at 1,551,307 to 1,551,591
ppiC
DVU1471: hspC - heat shock protein, Hsp20 family (TIGR), at 1,551,664 to 1,552,092
hspC
DVU1472: DVU1472 - ATP-dependent protease, putative (TIGR), at 1,552,089 to 1,554,554
DVU1472
Position (kb)
1549
1550
1551
1552 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1548.745 kb on - strand, within htrA at 1548.748 kb on + strand, within htrA at 1548.756 kb on - strand, within htrA at 1548.908 kb on + strand, within htrA at 1548.947 kb on + strand, within htrA at 1548.980 kb on + strand, within htrA at 1548.993 kb on - strand, within htrA at 1549.025 kb on + strand, within htrA at 1549.025 kb on + strand, within htrA at 1549.033 kb on - strand, within htrA at 1549.081 kb on + strand, within htrA at 1549.089 kb on - strand, within htrA at 1549.091 kb on + strand, within htrA at 1549.144 kb on + strand, within htrA at 1549.200 kb on - strand, within htrA at 1549.216 kb on + strand, within htrA at 1549.217 kb on - strand, within htrA at 1549.241 kb on + strand, within htrA at 1549.344 kb on - strand, within htrA at 1549.479 kb on - strand at 1549.583 kb on + strand at 1549.657 kb on - strand at 1549.816 kb on + strand at 1549.906 kb on - strand, within rpsA at 1550.018 kb on - strand, within rpsA at 1550.191 kb on + strand, within rpsA at 1550.283 kb on + strand, within rpsA at 1550.661 kb on - strand, within rpsA at 1550.672 kb on + strand, within rpsA at 1550.672 kb on - strand, within rpsA at 1550.942 kb on - strand, within rpsA at 1550.986 kb on - strand, within rpsA at 1550.986 kb on - strand, within rpsA at 1551.007 kb on + strand at 1551.104 kb on + strand at 1551.123 kb on + strand at 1551.123 kb on + strand at 1551.123 kb on + strand at 1551.123 kb on + strand at 1551.131 kb on - strand at 1551.133 kb on + strand at 1551.133 kb on + strand at 1551.327 kb on + strand at 1551.344 kb on + strand, within ppiC at 1551.478 kb on - strand, within ppiC at 1551.508 kb on + strand, within ppiC at 1551.530 kb on + strand, within ppiC at 1551.536 kb on + strand, within ppiC at 1551.536 kb on + strand, within ppiC at 1551.602 kb on + strand at 1551.658 kb on - strand at 1551.660 kb on + strand at 1551.660 kb on + strand at 1551.818 kb on - strand, within hspC at 1551.899 kb on + strand, within hspC at 1551.939 kb on - strand, within hspC at 1552.008 kb on - strand, within hspC at 1552.028 kb on - strand, within hspC at 1552.046 kb on + strand, within hspC at 1552.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Sulfate (60-50mM) with 0.1% Yeast Extract remove 1,548,745 - htrA DVU1468 0.46 +0.5 1,548,748 + htrA DVU1468 0.46 -0.4 1,548,756 - htrA DVU1468 0.47 +0.0 1,548,908 + htrA DVU1468 0.57 +0.1 1,548,947 + htrA DVU1468 0.60 +0.1 1,548,980 + htrA DVU1468 0.62 -0.4 1,548,993 - htrA DVU1468 0.63 +0.0 1,549,025 + htrA DVU1468 0.65 -0.3 1,549,025 + htrA DVU1468 0.65 -0.6 1,549,033 - htrA DVU1468 0.66 +1.2 1,549,081 + htrA DVU1468 0.69 -1.0 1,549,089 - htrA DVU1468 0.70 +2.2 1,549,091 + htrA DVU1468 0.70 -0.7 1,549,144 + htrA DVU1468 0.73 +0.3 1,549,200 - htrA DVU1468 0.77 +0.2 1,549,216 + htrA DVU1468 0.78 -0.5 1,549,217 - htrA DVU1468 0.78 -0.1 1,549,241 + htrA DVU1468 0.80 +0.6 1,549,344 - htrA DVU1468 0.87 +0.0 1,549,479 - +2.9 1,549,583 + -0.4 1,549,657 - -0.0 1,549,816 + -1.0 1,549,906 - rpsA DVU1469 0.15 +0.7 1,550,018 - rpsA DVU1469 0.22 -0.3 1,550,191 + rpsA DVU1469 0.34 -1.5 1,550,283 + rpsA DVU1469 0.41 -0.2 1,550,661 - rpsA DVU1469 0.66 -0.8 1,550,672 + rpsA DVU1469 0.67 -1.7 1,550,672 - rpsA DVU1469 0.67 +0.0 1,550,942 - rpsA DVU1469 0.86 -0.6 1,550,986 - rpsA DVU1469 0.89 +0.6 1,550,986 - rpsA DVU1469 0.89 -1.4 1,551,007 + -0.6 1,551,104 + -1.2 1,551,123 + +0.9 1,551,123 + -0.6 1,551,123 + +1.4 1,551,123 + +0.0 1,551,131 - -0.6 1,551,133 + -0.7 1,551,133 + -0.8 1,551,327 + -0.5 1,551,344 + ppiC DVU1470 0.13 -1.0 1,551,478 - ppiC DVU1470 0.60 +0.1 1,551,508 + ppiC DVU1470 0.71 -0.9 1,551,530 + ppiC DVU1470 0.78 -1.0 1,551,536 + ppiC DVU1470 0.80 -0.2 1,551,536 + ppiC DVU1470 0.80 +2.2 1,551,602 + -0.9 1,551,658 - +0.4 1,551,660 + -0.4 1,551,660 + -0.4 1,551,818 - hspC DVU1471 0.36 +0.3 1,551,899 + hspC DVU1471 0.55 -1.6 1,551,939 - hspC DVU1471 0.64 +0.0 1,552,008 - hspC DVU1471 0.80 -0.4 1,552,028 - hspC DVU1471 0.85 +0.9 1,552,046 + hspC DVU1471 0.89 -0.1 1,552,054 - +0.2
Or see this region's nucleotide sequence