Experiment: MoYLS4 with Molybdate 100uM and Tungstate 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hynA-1 and hupD are separated by 368 nucleotides hupD and hypC are separated by 11 nucleotides hypC and tesA are separated by 17 nucleotides tesA and DVU1926 overlap by 19 nucleotides
DVU1922: hynA-1 - periplasmic [NiFe] hydrogenase, large subunit, isozyme 1 (TIGR), at 1,995,648 to 1,997,348
hynA-1
DVU1923: hupD - hydrogenase expression/formation protein HupD (TIGR), at 1,997,717 to 1,998,214
hupD
DVU1924: hypC - hydrogenase assembly chaperone hypC/hupF (TIGR), at 1,998,226 to 1,998,477
hypC
DVU1925: tesA - lipase, GDSL family (TIGR), at 1,998,495 to 1,999,136
tesA
DVU1926: DVU1926 - conserved hypothetical protein (TIGR), at 1,999,118 to 1,999,606
DVU1926
Position (kb)
1997
1998
1999 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1996.731 kb on + strand, within hynA-1 at 1996.833 kb on - strand, within hynA-1 at 1996.847 kb on + strand, within hynA-1 at 1996.855 kb on - strand, within hynA-1 at 1996.904 kb on + strand, within hynA-1 at 1996.992 kb on - strand, within hynA-1 at 1997.027 kb on + strand, within hynA-1 at 1997.194 kb on - strand at 1997.269 kb on + strand at 1997.270 kb on - strand at 1997.280 kb on + strand at 1997.295 kb on - strand at 1997.318 kb on - strand at 1997.355 kb on + strand at 1997.355 kb on + strand at 1997.355 kb on + strand at 1997.355 kb on + strand at 1997.355 kb on + strand at 1997.363 kb on - strand at 1997.363 kb on - strand at 1997.363 kb on - strand at 1997.376 kb on - strand at 1997.491 kb on - strand at 1997.554 kb on - strand at 1997.591 kb on - strand at 1997.707 kb on - strand at 1997.709 kb on - strand at 1997.731 kb on + strand at 1997.772 kb on - strand, within hupD at 1997.779 kb on - strand, within hupD at 1997.779 kb on - strand, within hupD at 1997.820 kb on - strand, within hupD at 1997.821 kb on - strand, within hupD at 1997.888 kb on - strand, within hupD at 1997.890 kb on - strand, within hupD at 1997.900 kb on - strand, within hupD at 1997.961 kb on + strand, within hupD at 1997.969 kb on - strand, within hupD at 1998.022 kb on - strand, within hupD at 1998.024 kb on + strand, within hupD at 1998.034 kb on - strand, within hupD at 1998.121 kb on - strand, within hupD at 1998.542 kb on + strand at 1998.542 kb on + strand at 1998.601 kb on + strand, within tesA at 1998.628 kb on + strand, within tesA at 1998.795 kb on + strand, within tesA at 1998.854 kb on + strand, within tesA at 1998.862 kb on - strand, within tesA at 1998.862 kb on - strand, within tesA at 1998.862 kb on - strand, within tesA at 1998.914 kb on + strand, within tesA at 1998.914 kb on + strand, within tesA at 1998.914 kb on + strand, within tesA at 1998.914 kb on + strand, within tesA at 1998.922 kb on - strand, within tesA at 1999.029 kb on + strand, within tesA
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Molybdate 100uM and Tungstate 0.5mM remove 1,996,731 + hynA-1 DVU1922 0.64 -0.2 1,996,833 - hynA-1 DVU1922 0.70 -0.9 1,996,847 + hynA-1 DVU1922 0.70 -0.2 1,996,855 - hynA-1 DVU1922 0.71 -1.0 1,996,904 + hynA-1 DVU1922 0.74 -0.1 1,996,992 - hynA-1 DVU1922 0.79 -0.3 1,997,027 + hynA-1 DVU1922 0.81 +0.2 1,997,194 - +1.1 1,997,269 + +1.6 1,997,270 - -0.6 1,997,280 + +0.6 1,997,295 - -0.9 1,997,318 - +0.5 1,997,355 + +0.3 1,997,355 + -0.4 1,997,355 + -0.4 1,997,355 + -0.2 1,997,355 + -1.5 1,997,363 - -0.8 1,997,363 - +1.6 1,997,363 - -0.9 1,997,376 - -1.3 1,997,491 - +0.5 1,997,554 - -0.5 1,997,591 - -0.0 1,997,707 - -0.4 1,997,709 - +1.4 1,997,731 + -0.4 1,997,772 - hupD DVU1923 0.11 -0.2 1,997,779 - hupD DVU1923 0.12 -1.1 1,997,779 - hupD DVU1923 0.12 -1.5 1,997,820 - hupD DVU1923 0.21 -0.9 1,997,821 - hupD DVU1923 0.21 -1.7 1,997,888 - hupD DVU1923 0.34 -1.0 1,997,890 - hupD DVU1923 0.35 -0.3 1,997,900 - hupD DVU1923 0.37 -1.6 1,997,961 + hupD DVU1923 0.49 -1.5 1,997,969 - hupD DVU1923 0.51 -2.0 1,998,022 - hupD DVU1923 0.61 -1.5 1,998,024 + hupD DVU1923 0.62 -0.2 1,998,034 - hupD DVU1923 0.64 -1.3 1,998,121 - hupD DVU1923 0.81 -1.9 1,998,542 + +0.6 1,998,542 + -1.2 1,998,601 + tesA DVU1925 0.17 -0.2 1,998,628 + tesA DVU1925 0.21 -0.3 1,998,795 + tesA DVU1925 0.47 +3.0 1,998,854 + tesA DVU1925 0.56 -0.5 1,998,862 - tesA DVU1925 0.57 +0.5 1,998,862 - tesA DVU1925 0.57 -0.5 1,998,862 - tesA DVU1925 0.57 -0.5 1,998,914 + tesA DVU1925 0.65 -2.8 1,998,914 + tesA DVU1925 0.65 +0.3 1,998,914 + tesA DVU1925 0.65 -0.5 1,998,914 + tesA DVU1925 0.65 -0.4 1,998,922 - tesA DVU1925 0.67 +0.6 1,999,029 + tesA DVU1925 0.83 -0.4
Or see this region's nucleotide sequence