Experiment: MoLS4 with L-Phenylalanine and L-Tyrosine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt modA and DVU0179 are separated by 204 nucleotides DVU0179 and modC overlap by 14 nucleotides modC and modB overlap by 4 nucleotides
DVU0177: modA - molybdenum ABC transporter, periplasmic molybdenum-binding protein (TIGR), at 222,194 to 223,003
modA
DVU0179: DVU0179 - molybdenum-pterin binding domain protein/site-specific recombinase, phage integrase family (TIGR), at 223,208 to 224,605
DVU0179
DVU0180: modC - ATP-binding component of molybdate ABC transporter (Dmitry Rodionov), at 224,592 to 225,353
modC
DVU0181: modB - molybdenum ABC transporter, permease protein (TIGR), at 225,350 to 226,051
modB
Position (kb) 223
224
225 Strain fitness (log2 ratio) -2
-1
0
1
2 at 222.354 kb on + strand, within modA at 222.397 kb on - strand, within modA at 222.419 kb on - strand, within modA at 222.425 kb on + strand, within modA at 222.433 kb on - strand, within modA at 222.474 kb on - strand, within modA at 222.482 kb on - strand, within modA at 222.482 kb on - strand, within modA at 222.486 kb on - strand, within modA at 222.539 kb on - strand, within modA at 222.570 kb on + strand, within modA at 222.625 kb on + strand, within modA at 222.660 kb on + strand, within modA at 222.771 kb on - strand, within modA at 222.910 kb on - strand, within modA at 222.936 kb on - strand at 222.939 kb on + strand at 222.939 kb on + strand at 222.942 kb on - strand at 222.944 kb on - strand at 222.947 kb on - strand at 222.947 kb on - strand at 222.947 kb on - strand at 222.951 kb on + strand at 222.951 kb on + strand at 222.951 kb on + strand at 222.951 kb on + strand at 222.951 kb on + strand at 222.951 kb on + strand at 222.957 kb on - strand at 222.959 kb on - strand at 222.959 kb on - strand at 222.959 kb on - strand at 222.961 kb on + strand at 223.007 kb on - strand at 223.010 kb on + strand at 223.076 kb on + strand at 223.084 kb on - strand at 223.104 kb on + strand at 223.154 kb on - strand at 223.154 kb on - strand at 223.320 kb on + strand at 223.374 kb on + strand, within DVU0179 at 223.399 kb on + strand, within DVU0179 at 223.426 kb on + strand, within DVU0179 at 223.426 kb on + strand, within DVU0179 at 223.426 kb on + strand, within DVU0179 at 223.441 kb on - strand, within DVU0179 at 223.441 kb on - strand, within DVU0179 at 223.450 kb on + strand, within DVU0179 at 223.458 kb on - strand, within DVU0179 at 223.458 kb on - strand, within DVU0179 at 223.461 kb on + strand, within DVU0179 at 223.503 kb on - strand, within DVU0179 at 223.576 kb on + strand, within DVU0179 at 223.584 kb on - strand, within DVU0179 at 223.655 kb on - strand, within DVU0179 at 223.710 kb on - strand, within DVU0179 at 223.839 kb on + strand, within DVU0179 at 223.840 kb on + strand, within DVU0179 at 223.840 kb on + strand, within DVU0179 at 223.850 kb on + strand, within DVU0179 at 223.895 kb on + strand, within DVU0179 at 223.901 kb on - strand, within DVU0179 at 223.903 kb on - strand, within DVU0179 at 223.905 kb on + strand, within DVU0179 at 223.931 kb on + strand, within DVU0179 at 224.009 kb on - strand, within DVU0179 at 224.056 kb on + strand, within DVU0179 at 224.078 kb on + strand, within DVU0179 at 224.087 kb on + strand, within DVU0179 at 224.196 kb on - strand, within DVU0179 at 224.229 kb on - strand, within DVU0179 at 224.276 kb on + strand, within DVU0179 at 224.380 kb on - strand, within DVU0179 at 224.477 kb on + strand at 224.490 kb on - strand at 224.525 kb on - strand at 224.529 kb on - strand at 224.562 kb on - strand at 224.567 kb on - strand at 224.567 kb on - strand at 224.569 kb on + strand at 224.577 kb on - strand at 224.624 kb on + strand at 224.670 kb on + strand, within modC at 224.690 kb on + strand, within modC at 224.730 kb on + strand, within modC at 224.756 kb on - strand, within modC at 224.825 kb on + strand, within modC at 224.856 kb on + strand, within modC at 224.880 kb on + strand, within modC at 224.887 kb on + strand, within modC at 224.895 kb on - strand, within modC at 224.905 kb on - strand, within modC at 225.074 kb on + strand, within modC at 225.081 kb on + strand, within modC at 225.081 kb on + strand, within modC at 225.089 kb on - strand, within modC at 225.159 kb on - strand, within modC at 225.159 kb on - strand, within modC at 225.263 kb on - strand, within modC at 225.320 kb on - strand at 225.376 kb on + strand at 225.382 kb on - strand at 225.402 kb on - strand at 225.415 kb on + strand at 225.437 kb on - strand, within modB at 225.439 kb on - strand, within modB at 225.489 kb on - strand, within modB at 225.495 kb on - strand, within modB at 225.549 kb on + strand, within modB
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Phenylalanine and L-Tyrosine as nutrient 10mM remove 222,354 + modA DVU0177 0.20 -0.2 222,397 - modA DVU0177 0.25 +0.1 222,419 - modA DVU0177 0.28 +0.0 222,425 + modA DVU0177 0.29 -0.4 222,433 - modA DVU0177 0.30 -0.2 222,474 - modA DVU0177 0.35 -0.1 222,482 - modA DVU0177 0.36 -0.5 222,482 - modA DVU0177 0.36 -0.3 222,486 - modA DVU0177 0.36 -0.2 222,539 - modA DVU0177 0.43 +0.8 222,570 + modA DVU0177 0.46 -1.5 222,625 + modA DVU0177 0.53 -0.2 222,660 + modA DVU0177 0.58 +0.9 222,771 - modA DVU0177 0.71 +1.1 222,910 - modA DVU0177 0.88 +1.0 222,936 - +1.2 222,939 + -0.5 222,939 + -0.4 222,942 - +1.2 222,944 - -0.8 222,947 - -0.0 222,947 - -2.0 222,947 - +0.1 222,951 + +1.0 222,951 + +0.5 222,951 + -0.2 222,951 + +0.0 222,951 + -0.4 222,951 + -0.8 222,957 - -0.3 222,959 - -1.1 222,959 - +0.4 222,959 - -0.5 222,961 + -1.2 223,007 - -0.2 223,010 + +1.4 223,076 + +0.0 223,084 - -0.8 223,104 + -0.4 223,154 - -0.6 223,154 - +0.1 223,320 + -0.7 223,374 + DVU0179 0.12 -0.8 223,399 + DVU0179 0.14 +0.0 223,426 + DVU0179 0.16 +1.9 223,426 + DVU0179 0.16 -0.1 223,426 + DVU0179 0.16 +1.7 223,441 - DVU0179 0.17 +1.2 223,441 - DVU0179 0.17 -2.4 223,450 + DVU0179 0.17 +2.2 223,458 - DVU0179 0.18 -1.5 223,458 - DVU0179 0.18 -0.1 223,461 + DVU0179 0.18 +0.2 223,503 - DVU0179 0.21 -0.2 223,576 + DVU0179 0.26 -0.0 223,584 - DVU0179 0.27 -1.3 223,655 - DVU0179 0.32 +0.2 223,710 - DVU0179 0.36 -0.3 223,839 + DVU0179 0.45 -0.4 223,840 + DVU0179 0.45 +0.0 223,840 + DVU0179 0.45 +0.5 223,850 + DVU0179 0.46 +0.6 223,895 + DVU0179 0.49 +1.2 223,901 - DVU0179 0.50 +0.7 223,903 - DVU0179 0.50 -0.1 223,905 + DVU0179 0.50 +0.7 223,931 + DVU0179 0.52 +0.6 224,009 - DVU0179 0.57 +0.3 224,056 + DVU0179 0.61 -0.3 224,078 + DVU0179 0.62 +0.3 224,087 + DVU0179 0.63 +0.2 224,196 - DVU0179 0.71 -0.0 224,229 - DVU0179 0.73 +0.3 224,276 + DVU0179 0.76 -0.2 224,380 - DVU0179 0.84 +0.2 224,477 + -0.3 224,490 - -0.0 224,525 - +0.2 224,529 - +0.7 224,562 - -0.4 224,567 - -0.0 224,567 - +0.2 224,569 + +0.4 224,577 - -1.4 224,624 + -0.4 224,670 + modC DVU0180 0.10 -0.1 224,690 + modC DVU0180 0.13 -0.4 224,730 + modC DVU0180 0.18 -0.3 224,756 - modC DVU0180 0.22 -0.5 224,825 + modC DVU0180 0.31 -0.6 224,856 + modC DVU0180 0.35 -1.2 224,880 + modC DVU0180 0.38 -0.5 224,887 + modC DVU0180 0.39 +0.0 224,895 - modC DVU0180 0.40 -0.0 224,905 - modC DVU0180 0.41 -0.6 225,074 + modC DVU0180 0.63 -0.0 225,081 + modC DVU0180 0.64 -0.5 225,081 + modC DVU0180 0.64 -1.7 225,089 - modC DVU0180 0.65 +0.0 225,159 - modC DVU0180 0.74 -0.1 225,159 - modC DVU0180 0.74 -0.1 225,263 - modC DVU0180 0.88 -0.1 225,320 - -0.7 225,376 + -0.4 225,382 - -0.4 225,402 - -0.3 225,415 + -0.1 225,437 - modB DVU0181 0.12 -0.4 225,439 - modB DVU0181 0.13 -1.0 225,489 - modB DVU0181 0.20 +0.3 225,495 - modB DVU0181 0.21 +0.5 225,549 + modB DVU0181 0.28 -0.4
Or see this region's nucleotide sequence