Experiment: Supernatant; Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (~7.5 mM 3-keto-trehalose) 0.5x (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3216 and BT3217 are separated by 30 nucleotides BT3217 and BT3218 overlap by 4 nucleotides BT3218 and BT3219 are separated by 207 nucleotides
BT3216: BT3216 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine (NCBI ptt file), at 4,107,989 to 4,108,471
BT3216
BT3217: BT3217 - hypothetical protein (NCBI ptt file), at 4,108,502 to 4,108,693
BT3217
BT3218: BT3218 - hypothetical protein (NCBI ptt file), at 4,108,690 to 4,108,923
BT3218
BT3219: BT3219 - S-adenosylmethionine synthetase (NCBI ptt file), at 4,109,131 to 4,110,423
BT3219
Position (kb)
4109
4110
4111 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4108.563 kb on - strand, within BT3217 at 4108.571 kb on + strand, within BT3217 at 4108.571 kb on + strand, within BT3217 at 4108.572 kb on - strand, within BT3217 at 4108.583 kb on - strand, within BT3217 at 4108.613 kb on + strand, within BT3217 at 4108.614 kb on - strand, within BT3217 at 4108.624 kb on - strand, within BT3217 at 4108.643 kb on + strand, within BT3217 at 4108.648 kb on - strand, within BT3217 at 4108.687 kb on + strand at 4108.694 kb on - strand at 4108.708 kb on - strand at 4108.729 kb on + strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.787 kb on + strand, within BT3218 at 4108.788 kb on - strand, within BT3218 at 4108.868 kb on + strand, within BT3218 at 4108.877 kb on - strand, within BT3218 at 4108.877 kb on - strand, within BT3218 at 4108.908 kb on + strand at 4108.909 kb on - strand at 4108.911 kb on - strand at 4108.911 kb on - strand at 4108.917 kb on + strand at 4108.939 kb on - strand at 4108.958 kb on + strand at 4109.110 kb on + strand at 4109.110 kb on + strand at 4110.444 kb on + strand at 4110.445 kb on - strand at 4110.467 kb on + strand at 4110.537 kb on + strand at 4110.545 kb on - strand at 4110.551 kb on + strand at 4110.577 kb on + strand at 4110.577 kb on + strand at 4110.584 kb on + strand at 4110.585 kb on - strand at 4110.593 kb on + strand at 4110.605 kb on - strand at 4110.629 kb on - strand at 4110.680 kb on - strand at 4110.682 kb on - strand at 4110.687 kb on - strand at 4110.687 kb on - strand at 4110.731 kb on - strand at 4110.739 kb on + strand at 4110.739 kb on + strand at 4110.752 kb on + strand at 4110.775 kb on - strand at 4110.805 kb on + strand at 4110.810 kb on + strand at 4110.810 kb on + strand at 4110.811 kb on - strand at 4110.815 kb on + strand at 4110.816 kb on - strand at 4110.819 kb on - strand at 4110.819 kb on - strand at 4110.903 kb on + strand at 4110.967 kb on + strand at 4110.968 kb on - strand at 4111.017 kb on + strand at 4111.146 kb on + strand at 4111.147 kb on - strand at 4111.165 kb on - strand at 4111.207 kb on - strand at 4111.208 kb on + strand at 4111.208 kb on + strand at 4111.209 kb on - strand at 4111.227 kb on + strand at 4111.258 kb on - strand at 4111.263 kb on - strand at 4111.335 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (~7.5 mM 3-keto-trehalose) 0.5x (C) remove 4,108,563 - BT3217 0.32 -1.3 4,108,571 + BT3217 0.36 -0.7 4,108,571 + BT3217 0.36 -0.1 4,108,572 - BT3217 0.36 -0.1 4,108,583 - BT3217 0.42 -2.2 4,108,613 + BT3217 0.58 -0.9 4,108,614 - BT3217 0.58 -2.1 4,108,624 - BT3217 0.64 -0.9 4,108,643 + BT3217 0.73 -3.1 4,108,648 - BT3217 0.76 +0.4 4,108,687 + -0.7 4,108,694 - -0.2 4,108,708 - -2.7 4,108,729 + BT3218 0.17 -1.9 4,108,756 - BT3218 0.28 -1.6 4,108,756 - BT3218 0.28 -2.9 4,108,756 - BT3218 0.28 -0.7 4,108,787 + BT3218 0.41 -1.0 4,108,788 - BT3218 0.42 +1.1 4,108,868 + BT3218 0.76 -2.7 4,108,877 - BT3218 0.80 -2.1 4,108,877 - BT3218 0.80 -0.5 4,108,908 + -0.8 4,108,909 - -1.8 4,108,911 - -0.2 4,108,911 - -0.0 4,108,917 + +0.1 4,108,939 - -1.8 4,108,958 + -1.2 4,109,110 + -1.6 4,109,110 + +0.5 4,110,444 + -0.1 4,110,445 - +0.0 4,110,467 + -2.6 4,110,537 + -1.6 4,110,545 - -1.1 4,110,551 + +0.4 4,110,577 + -0.7 4,110,577 + -1.0 4,110,584 + +1.9 4,110,585 - -0.5 4,110,593 + -0.1 4,110,605 - -0.9 4,110,629 - -0.2 4,110,680 - -2.3 4,110,682 - -2.1 4,110,687 - -0.6 4,110,687 - -1.6 4,110,731 - -1.6 4,110,739 + -1.9 4,110,739 + -1.5 4,110,752 + +1.4 4,110,775 - +0.5 4,110,805 + -0.9 4,110,810 + +0.4 4,110,810 + +0.1 4,110,811 - -0.7 4,110,815 + -0.4 4,110,816 - -0.1 4,110,819 - -1.9 4,110,819 - -0.3 4,110,903 + +0.4 4,110,967 + -1.1 4,110,968 - -2.1 4,111,017 + -1.6 4,111,146 + +0.6 4,111,147 - -0.9 4,111,165 - +0.2 4,111,207 - +0.3 4,111,208 + -0.6 4,111,208 + +0.1 4,111,209 - +0.4 4,111,227 + -1.1 4,111,258 - -0.8 4,111,263 - -0.3 4,111,335 + +0.7
Or see this region's nucleotide sequence