Experiment: Supernatant; Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (~7.5 mM 3-keto-trehalose) 0.5x (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3214 and BT3215 are separated by 16 nucleotides BT3215 and BT3216 are separated by 7 nucleotides BT3216 and BT3217 are separated by 30 nucleotides BT3217 and BT3218 overlap by 4 nucleotides
BT3214: BT3214 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 4,106,571 to 4,107,629
BT3214
BT3215: BT3215 - conserved hypothetical protein (NCBI ptt file), at 4,107,646 to 4,107,981
BT3215
BT3216: BT3216 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine (NCBI ptt file), at 4,107,989 to 4,108,471
BT3216
BT3217: BT3217 - hypothetical protein (NCBI ptt file), at 4,108,502 to 4,108,693
BT3217
BT3218: BT3218 - hypothetical protein (NCBI ptt file), at 4,108,690 to 4,108,923
BT3218
Position (kb)
4107
4108 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4107.684 kb on + strand, within BT3215 at 4107.684 kb on + strand at 4107.712 kb on + strand, within BT3215 at 4107.734 kb on + strand, within BT3215 at 4107.907 kb on + strand, within BT3215 at 4107.932 kb on + strand, within BT3215 at 4107.932 kb on + strand, within BT3215 at 4107.974 kb on + strand at 4107.974 kb on + strand at 4108.563 kb on - strand, within BT3217 at 4108.571 kb on + strand, within BT3217 at 4108.571 kb on + strand, within BT3217 at 4108.572 kb on - strand, within BT3217 at 4108.583 kb on - strand, within BT3217 at 4108.613 kb on + strand, within BT3217 at 4108.614 kb on - strand, within BT3217 at 4108.624 kb on - strand, within BT3217 at 4108.643 kb on + strand, within BT3217 at 4108.648 kb on - strand, within BT3217 at 4108.687 kb on + strand at 4108.694 kb on - strand at 4108.708 kb on - strand at 4108.729 kb on + strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.787 kb on + strand, within BT3218 at 4108.788 kb on - strand, within BT3218 at 4108.868 kb on + strand, within BT3218 at 4108.877 kb on - strand, within BT3218 at 4108.877 kb on - strand, within BT3218 at 4108.908 kb on + strand at 4108.909 kb on - strand at 4108.911 kb on - strand at 4108.911 kb on - strand at 4108.917 kb on + strand at 4108.939 kb on - strand at 4108.958 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (~7.5 mM 3-keto-trehalose) 0.5x (C) remove 4,107,684 + BT3215 0.11 -1.2 4,107,684 + -0.6 4,107,712 + BT3215 0.20 -0.6 4,107,734 + BT3215 0.26 -0.2 4,107,907 + BT3215 0.78 +0.0 4,107,932 + BT3215 0.85 +1.9 4,107,932 + BT3215 0.85 -0.1 4,107,974 + -1.2 4,107,974 + +1.0 4,108,563 - BT3217 0.32 -1.3 4,108,571 + BT3217 0.36 -0.7 4,108,571 + BT3217 0.36 -0.1 4,108,572 - BT3217 0.36 -0.1 4,108,583 - BT3217 0.42 -2.2 4,108,613 + BT3217 0.58 -0.9 4,108,614 - BT3217 0.58 -2.1 4,108,624 - BT3217 0.64 -0.9 4,108,643 + BT3217 0.73 -3.1 4,108,648 - BT3217 0.76 +0.4 4,108,687 + -0.7 4,108,694 - -0.2 4,108,708 - -2.7 4,108,729 + BT3218 0.17 -1.9 4,108,756 - BT3218 0.28 -1.6 4,108,756 - BT3218 0.28 -2.9 4,108,756 - BT3218 0.28 -0.7 4,108,787 + BT3218 0.41 -1.0 4,108,788 - BT3218 0.42 +1.1 4,108,868 + BT3218 0.76 -2.7 4,108,877 - BT3218 0.80 -2.1 4,108,877 - BT3218 0.80 -0.5 4,108,908 + -0.8 4,108,909 - -1.8 4,108,911 - -0.2 4,108,911 - -0.0 4,108,917 + +0.1 4,108,939 - -1.8 4,108,958 + -1.2
Or see this region's nucleotide sequence