Experiment: Supernatant; Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (~7.5 mM 3-keto-trehalose) 0.5x (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1381 and tRNA-Asp are separated by 446 nucleotides tRNA-Asp and tRNA-Asp are separated by 53 nucleotides tRNA-Asp and BT1382 are separated by 253 nucleotides BT1382 and BT1383 are separated by 206 nucleotides
BT1381: BT1381 - formyltetrahydrofolate deformylase (NCBI ptt file), at 1,708,512 to 1,709,366
BT1381
BT_t25: tRNA-Asp - tRNA-Asp (RefSeq), at 1,709,813 to 1,709,886
tRNA-Asp
BT_t26: tRNA-Asp - tRNA-Asp (RefSeq), at 1,709,940 to 1,710,013
tRNA-Asp
BT1382: BT1382 - conserved hypothetical protein (NCBI ptt file), at 1,710,267 to 1,710,860
BT1382
BT1383: BT1383 - oxidoreductase, aldo/keto reductase (NCBI ptt file), at 1,711,067 to 1,711,903
BT1383
Position (kb)
1710
1711 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1709.270 kb on - strand, within BT1381 at 1709.276 kb on + strand, within BT1381 at 1709.360 kb on + strand at 1709.513 kb on + strand at 1709.588 kb on - strand at 1709.693 kb on + strand at 1709.898 kb on + strand at 1710.110 kb on - strand at 1710.133 kb on + strand at 1710.168 kb on + strand at 1710.178 kb on + strand at 1710.179 kb on - strand at 1710.182 kb on + strand at 1710.194 kb on + strand at 1710.247 kb on + strand at 1710.292 kb on + strand at 1710.323 kb on + strand at 1710.326 kb on - strand at 1710.351 kb on - strand, within BT1382 at 1710.373 kb on - strand, within BT1382 at 1710.433 kb on + strand, within BT1382 at 1710.487 kb on + strand, within BT1382 at 1710.530 kb on - strand, within BT1382 at 1710.561 kb on - strand, within BT1382 at 1710.605 kb on + strand, within BT1382 at 1710.605 kb on + strand, within BT1382 at 1710.628 kb on + strand, within BT1382 at 1710.644 kb on + strand, within BT1382 at 1710.654 kb on + strand, within BT1382 at 1710.681 kb on + strand, within BT1382 at 1710.684 kb on - strand, within BT1382 at 1710.684 kb on - strand, within BT1382 at 1710.684 kb on - strand, within BT1382 at 1710.699 kb on + strand, within BT1382 at 1710.718 kb on + strand, within BT1382 at 1710.778 kb on + strand, within BT1382 at 1710.779 kb on - strand, within BT1382 at 1710.781 kb on + strand, within BT1382 at 1710.783 kb on + strand, within BT1382 at 1710.784 kb on - strand, within BT1382 at 1710.791 kb on - strand, within BT1382 at 1710.800 kb on + strand, within BT1382 at 1710.932 kb on - strand at 1710.960 kb on - strand at 1710.981 kb on - strand at 1711.014 kb on - strand at 1711.052 kb on + strand at 1711.056 kb on + strand at 1711.057 kb on - strand at 1711.075 kb on + strand at 1711.075 kb on + strand at 1711.454 kb on - strand, within BT1383 at 1711.557 kb on + strand, within BT1383 at 1711.557 kb on + strand, within BT1383 at 1711.557 kb on + strand, within BT1383 at 1711.558 kb on - strand, within BT1383 at 1711.558 kb on - strand, within BT1383 at 1711.566 kb on + strand, within BT1383 at 1711.570 kb on - strand, within BT1383 at 1711.625 kb on - strand, within BT1383 at 1711.715 kb on + strand, within BT1383 at 1711.761 kb on + strand, within BT1383 at 1711.762 kb on - strand, within BT1383 at 1711.762 kb on - strand, within BT1383 at 1711.780 kb on + strand, within BT1383 at 1711.846 kb on - strand at 1711.848 kb on - strand at 1711.848 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (~7.5 mM 3-keto-trehalose) 0.5x (C) remove 1,709,270 - BT1381 0.89 -2.0 1,709,276 + BT1381 0.89 -1.6 1,709,360 + -1.4 1,709,513 + +1.8 1,709,588 - -1.1 1,709,693 + -1.9 1,709,898 + -1.0 1,710,110 - -1.1 1,710,133 + -0.4 1,710,168 + -0.5 1,710,178 + -1.4 1,710,179 - -2.6 1,710,182 + +0.3 1,710,194 + +0.8 1,710,247 + +2.0 1,710,292 + +1.6 1,710,323 + -2.5 1,710,326 - +1.6 1,710,351 - BT1382 0.14 +0.8 1,710,373 - BT1382 0.18 -1.0 1,710,433 + BT1382 0.28 -1.2 1,710,487 + BT1382 0.37 -2.0 1,710,530 - BT1382 0.44 +0.6 1,710,561 - BT1382 0.49 -3.4 1,710,605 + BT1382 0.57 -0.7 1,710,605 + BT1382 0.57 -1.1 1,710,628 + BT1382 0.61 -2.6 1,710,644 + BT1382 0.63 -1.2 1,710,654 + BT1382 0.65 +1.1 1,710,681 + BT1382 0.70 -0.0 1,710,684 - BT1382 0.70 -0.7 1,710,684 - BT1382 0.70 -1.9 1,710,684 - BT1382 0.70 -2.7 1,710,699 + BT1382 0.73 -2.3 1,710,718 + BT1382 0.76 -1.2 1,710,778 + BT1382 0.86 -0.4 1,710,779 - BT1382 0.86 -0.5 1,710,781 + BT1382 0.87 -1.0 1,710,783 + BT1382 0.87 -2.1 1,710,784 - BT1382 0.87 -0.2 1,710,791 - BT1382 0.88 -1.2 1,710,800 + BT1382 0.90 -0.5 1,710,932 - +1.6 1,710,960 - -0.2 1,710,981 - -2.0 1,711,014 - -0.0 1,711,052 + -3.2 1,711,056 + +1.0 1,711,057 - -0.4 1,711,075 + -2.3 1,711,075 + +0.3 1,711,454 - BT1383 0.46 -1.7 1,711,557 + BT1383 0.59 -1.4 1,711,557 + BT1383 0.59 -3.0 1,711,557 + BT1383 0.59 -0.0 1,711,558 - BT1383 0.59 -0.0 1,711,558 - BT1383 0.59 +0.1 1,711,566 + BT1383 0.60 -0.7 1,711,570 - BT1383 0.60 +1.1 1,711,625 - BT1383 0.67 -2.7 1,711,715 + BT1383 0.77 -1.5 1,711,761 + BT1383 0.83 -1.7 1,711,762 - BT1383 0.83 +0.3 1,711,762 - BT1383 0.83 -0.7 1,711,780 + BT1383 0.85 +0.1 1,711,846 - +0.4 1,711,848 - -1.2 1,711,848 - -1.2
Or see this region's nucleotide sequence