Experiment: D-Trehalose 20mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1288 and BT1289 are separated by 23 nucleotides BT1289 and BT1290 overlap by 7 nucleotides BT1290 and BT1291 are separated by 35 nucleotides
BT1288: BT1288 - spermidine/putrescine ABC transporter (NCBI ptt file), at 1,604,650 to 1,605,999
BT1288
BT1289: BT1289 - putrescine transport system permease protein potI (NCBI ptt file), at 1,606,023 to 1,606,820
BT1289
BT1290: BT1290 - putrescine transport system permease protein potH (NCBI ptt file), at 1,606,814 to 1,607,590
BT1290
BT1291: BT1291 - spermidine/putrescine transport ATP-binding protein (NCBI ptt file), at 1,607,626 to 1,609,017
BT1291
Position (kb)
1606
1607 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1605.111 kb on - strand, within BT1288 at 1605.134 kb on + strand, within BT1288 at 1605.141 kb on + strand, within BT1288 at 1605.235 kb on - strand, within BT1288 at 1605.235 kb on - strand, within BT1288 at 1605.235 kb on - strand, within BT1288 at 1605.339 kb on + strand, within BT1288 at 1605.418 kb on - strand, within BT1288 at 1605.418 kb on - strand, within BT1288 at 1605.525 kb on - strand, within BT1288 at 1605.525 kb on - strand, within BT1288 at 1605.537 kb on - strand, within BT1288 at 1605.544 kb on - strand, within BT1288 at 1605.561 kb on + strand, within BT1288 at 1605.562 kb on - strand, within BT1288 at 1605.609 kb on + strand, within BT1288 at 1605.613 kb on - strand, within BT1288 at 1605.650 kb on + strand, within BT1288 at 1605.651 kb on - strand, within BT1288 at 1605.722 kb on + strand, within BT1288 at 1605.722 kb on + strand, within BT1288 at 1605.723 kb on - strand, within BT1288 at 1605.806 kb on + strand, within BT1288 at 1605.820 kb on - strand, within BT1288 at 1605.879 kb on + strand at 1605.882 kb on - strand at 1605.895 kb on - strand at 1605.896 kb on + strand at 1605.897 kb on - strand at 1605.897 kb on - strand at 1605.936 kb on - strand at 1605.936 kb on - strand at 1606.016 kb on + strand at 1606.017 kb on - strand at 1606.068 kb on + strand at 1606.159 kb on + strand, within BT1289 at 1606.159 kb on + strand, within BT1289 at 1606.159 kb on + strand, within BT1289 at 1606.159 kb on + strand, within BT1289 at 1606.160 kb on - strand, within BT1289 at 1606.196 kb on + strand, within BT1289 at 1606.196 kb on + strand, within BT1289 at 1606.304 kb on + strand, within BT1289 at 1606.378 kb on - strand, within BT1289 at 1606.430 kb on - strand, within BT1289 at 1606.459 kb on - strand, within BT1289 at 1606.487 kb on + strand, within BT1289 at 1606.508 kb on + strand, within BT1289 at 1606.509 kb on - strand, within BT1289 at 1606.590 kb on - strand, within BT1289 at 1606.625 kb on + strand, within BT1289 at 1606.739 kb on - strand, within BT1289 at 1606.767 kb on + strand at 1606.780 kb on - strand at 1606.816 kb on - strand at 1606.821 kb on - strand at 1606.841 kb on - strand at 1606.927 kb on + strand, within BT1290 at 1606.927 kb on + strand, within BT1290 at 1606.957 kb on + strand, within BT1290 at 1607.014 kb on + strand, within BT1290 at 1607.131 kb on - strand, within BT1290 at 1607.131 kb on - strand, within BT1290 at 1607.131 kb on - strand, within BT1290 at 1607.185 kb on + strand, within BT1290 at 1607.270 kb on - strand, within BT1290 at 1607.273 kb on + strand, within BT1290 at 1607.273 kb on + strand, within BT1290 at 1607.386 kb on + strand, within BT1290 at 1607.387 kb on - strand, within BT1290 at 1607.408 kb on - strand, within BT1290 at 1607.411 kb on + strand, within BT1290 at 1607.428 kb on + strand, within BT1290 at 1607.497 kb on + strand, within BT1290 at 1607.560 kb on - strand at 1607.594 kb on + strand at 1607.627 kb on + strand at 1607.640 kb on + strand at 1607.670 kb on + strand at 1607.756 kb on + strand at 1607.769 kb on + strand, within BT1291 at 1607.770 kb on - strand, within BT1291
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose 20mM (C) remove 1,605,111 - BT1288 0.34 -2.2 1,605,134 + BT1288 0.36 +0.6 1,605,141 + BT1288 0.36 -2.1 1,605,235 - BT1288 0.43 -0.4 1,605,235 - BT1288 0.43 -0.8 1,605,235 - BT1288 0.43 +0.2 1,605,339 + BT1288 0.51 -1.6 1,605,418 - BT1288 0.57 -2.0 1,605,418 - BT1288 0.57 -1.2 1,605,525 - BT1288 0.65 -0.9 1,605,525 - BT1288 0.65 -0.3 1,605,537 - BT1288 0.66 -3.8 1,605,544 - BT1288 0.66 -0.0 1,605,561 + BT1288 0.67 +0.6 1,605,562 - BT1288 0.68 -0.8 1,605,609 + BT1288 0.71 -0.6 1,605,613 - BT1288 0.71 -1.3 1,605,650 + BT1288 0.74 +0.1 1,605,651 - BT1288 0.74 +0.2 1,605,722 + BT1288 0.79 +0.3 1,605,722 + BT1288 0.79 -0.2 1,605,723 - BT1288 0.79 +0.1 1,605,806 + BT1288 0.86 +0.3 1,605,820 - BT1288 0.87 -0.4 1,605,879 + -1.6 1,605,882 - -0.2 1,605,895 - -3.1 1,605,896 + +0.7 1,605,897 - -1.9 1,605,897 - -0.8 1,605,936 - +0.3 1,605,936 - -1.2 1,606,016 + -2.2 1,606,017 - +0.1 1,606,068 + +0.7 1,606,159 + BT1289 0.17 +1.0 1,606,159 + BT1289 0.17 +1.9 1,606,159 + BT1289 0.17 -2.3 1,606,159 + BT1289 0.17 +1.8 1,606,160 - BT1289 0.17 +0.0 1,606,196 + BT1289 0.22 +0.3 1,606,196 + BT1289 0.22 +1.6 1,606,304 + BT1289 0.35 -0.7 1,606,378 - BT1289 0.44 +0.6 1,606,430 - BT1289 0.51 +0.7 1,606,459 - BT1289 0.55 +0.2 1,606,487 + BT1289 0.58 -1.7 1,606,508 + BT1289 0.61 -0.2 1,606,509 - BT1289 0.61 +1.4 1,606,590 - BT1289 0.71 -1.9 1,606,625 + BT1289 0.75 -1.4 1,606,739 - BT1289 0.90 -2.3 1,606,767 + +0.6 1,606,780 - -1.9 1,606,816 - -1.0 1,606,821 - -2.1 1,606,841 - +0.2 1,606,927 + BT1290 0.15 -2.1 1,606,927 + BT1290 0.15 -0.3 1,606,957 + BT1290 0.18 -1.9 1,607,014 + BT1290 0.26 -0.4 1,607,131 - BT1290 0.41 -2.5 1,607,131 - BT1290 0.41 -1.4 1,607,131 - BT1290 0.41 -0.4 1,607,185 + BT1290 0.48 -1.1 1,607,270 - BT1290 0.59 -1.7 1,607,273 + BT1290 0.59 -2.2 1,607,273 + BT1290 0.59 -0.8 1,607,386 + BT1290 0.74 -0.1 1,607,387 - BT1290 0.74 -2.7 1,607,408 - BT1290 0.76 -0.6 1,607,411 + BT1290 0.77 +1.1 1,607,428 + BT1290 0.79 -2.6 1,607,497 + BT1290 0.88 -2.8 1,607,560 - +0.3 1,607,594 + +0.1 1,607,627 + -1.2 1,607,640 + -2.2 1,607,670 + -1.7 1,607,756 + +0.3 1,607,769 + BT1291 0.10 -1.6 1,607,770 - BT1291 0.10 -0.8
Or see this region's nucleotide sequence