Experiment: Lactulose 20mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4454 and BT4455 are separated by 56 nucleotides BT4455 and BT4456 overlap by 4 nucleotides BT4456 and BT4457 overlap by 4 nucleotides BT4457 and BT4458 are separated by 203 nucleotides
BT4454: BT4454 - conserved hypothetical protein (NCBI ptt file), at 5,864,732 to 5,865,280
BT4454
BT4455: BT4455 - oxidoreductase, putative glycolate oxidase (NCBI ptt file), at 5,865,337 to 5,866,077
BT4455
BT4456: BT4456 - putative electron transport protein (NCBI ptt file), at 5,866,074 to 5,867,459
BT4456
BT4457: BT4457 - conserved hypothetical protein (NCBI ptt file), at 5,867,456 to 5,868,037
BT4457
BT4458: BT4458 - pyridoxine kinase (NCBI ptt file), at 5,868,241 to 5,869,113
BT4458
Position (kb)
5866
5867
5868 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5865.085 kb on - strand, within BT4454 at 5865.119 kb on + strand, within BT4454 at 5865.133 kb on + strand, within BT4454 at 5865.134 kb on - strand, within BT4454 at 5865.134 kb on - strand, within BT4454 at 5865.134 kb on - strand, within BT4454 at 5865.136 kb on - strand, within BT4454 at 5865.139 kb on + strand, within BT4454 at 5865.233 kb on - strand at 5865.344 kb on + strand at 5865.365 kb on - strand at 5865.375 kb on + strand at 5865.376 kb on - strand at 5865.429 kb on - strand, within BT4455 at 5865.650 kb on - strand, within BT4455 at 5865.759 kb on - strand, within BT4455 at 5865.783 kb on + strand, within BT4455 at 5865.793 kb on + strand, within BT4455 at 5865.856 kb on - strand, within BT4455 at 5866.239 kb on + strand, within BT4456 at 5866.335 kb on + strand, within BT4456 at 5866.336 kb on - strand, within BT4456 at 5866.351 kb on - strand, within BT4456 at 5866.371 kb on - strand, within BT4456 at 5866.375 kb on + strand, within BT4456 at 5866.375 kb on + strand, within BT4456 at 5866.376 kb on - strand, within BT4456 at 5866.376 kb on - strand, within BT4456 at 5866.376 kb on - strand, within BT4456 at 5866.460 kb on - strand, within BT4456 at 5866.481 kb on + strand, within BT4456 at 5866.482 kb on - strand, within BT4456 at 5866.608 kb on + strand, within BT4456 at 5866.712 kb on - strand, within BT4456 at 5866.746 kb on - strand, within BT4456 at 5866.746 kb on - strand, within BT4456 at 5866.869 kb on + strand, within BT4456 at 5866.869 kb on + strand, within BT4456 at 5866.870 kb on - strand, within BT4456 at 5866.870 kb on - strand, within BT4456 at 5866.870 kb on - strand, within BT4456 at 5866.871 kb on + strand, within BT4456 at 5866.872 kb on - strand, within BT4456 at 5866.908 kb on + strand, within BT4456 at 5866.917 kb on - strand, within BT4456 at 5866.920 kb on - strand, within BT4456 at 5866.964 kb on + strand, within BT4456 at 5867.007 kb on - strand, within BT4456 at 5867.007 kb on - strand, within BT4456 at 5867.043 kb on + strand, within BT4456 at 5867.044 kb on - strand, within BT4456 at 5867.044 kb on - strand, within BT4456 at 5867.050 kb on - strand, within BT4456 at 5867.054 kb on + strand, within BT4456 at 5867.110 kb on - strand, within BT4456 at 5867.139 kb on - strand, within BT4456 at 5867.246 kb on - strand, within BT4456 at 5867.347 kb on - strand at 5867.434 kb on + strand at 5867.435 kb on - strand at 5867.491 kb on + strand at 5867.491 kb on + strand at 5867.526 kb on + strand, within BT4457 at 5867.527 kb on - strand, within BT4457 at 5867.527 kb on - strand, within BT4457 at 5867.593 kb on - strand, within BT4457 at 5867.610 kb on + strand, within BT4457 at 5867.611 kb on - strand, within BT4457 at 5867.694 kb on + strand, within BT4457 at 5867.695 kb on - strand, within BT4457 at 5867.897 kb on + strand, within BT4457 at 5868.028 kb on - strand at 5868.087 kb on + strand at 5868.087 kb on + strand at 5868.123 kb on - strand at 5868.130 kb on - strand at 5868.173 kb on + strand at 5868.175 kb on + strand at 5868.177 kb on + strand at 5868.178 kb on - strand at 5868.178 kb on - strand at 5868.225 kb on - strand at 5868.225 kb on - strand at 5868.229 kb on - strand at 5868.231 kb on + strand at 5868.245 kb on - strand at 5868.253 kb on - strand at 5868.258 kb on - strand at 5868.384 kb on + strand, within BT4458
Per-strain Table
Position Strand Gene LocusTag Fraction Lactulose 20mM (C) remove 5,865,085 - BT4454 0.64 -0.5 5,865,119 + BT4454 0.70 -2.0 5,865,133 + BT4454 0.73 -0.8 5,865,134 - BT4454 0.73 +0.0 5,865,134 - BT4454 0.73 -1.0 5,865,134 - BT4454 0.73 +0.6 5,865,136 - BT4454 0.74 -2.0 5,865,139 + BT4454 0.74 -2.2 5,865,233 - -0.0 5,865,344 + -0.2 5,865,365 - -0.3 5,865,375 + -1.8 5,865,376 - -0.3 5,865,429 - BT4455 0.12 -1.1 5,865,650 - BT4455 0.42 -1.4 5,865,759 - BT4455 0.57 -0.1 5,865,783 + BT4455 0.60 -0.1 5,865,793 + BT4455 0.62 -1.9 5,865,856 - BT4455 0.70 +0.1 5,866,239 + BT4456 0.12 -0.6 5,866,335 + BT4456 0.19 +0.8 5,866,336 - BT4456 0.19 -1.1 5,866,351 - BT4456 0.20 -1.1 5,866,371 - BT4456 0.21 +1.0 5,866,375 + BT4456 0.22 -0.3 5,866,375 + BT4456 0.22 +0.5 5,866,376 - BT4456 0.22 -1.1 5,866,376 - BT4456 0.22 -3.2 5,866,376 - BT4456 0.22 -1.8 5,866,460 - BT4456 0.28 -1.8 5,866,481 + BT4456 0.29 -2.3 5,866,482 - BT4456 0.29 -4.2 5,866,608 + BT4456 0.39 -1.6 5,866,712 - BT4456 0.46 -1.3 5,866,746 - BT4456 0.48 -1.5 5,866,746 - BT4456 0.48 -0.6 5,866,869 + BT4456 0.57 -1.9 5,866,869 + BT4456 0.57 -0.9 5,866,870 - BT4456 0.57 -1.2 5,866,870 - BT4456 0.57 -1.6 5,866,870 - BT4456 0.57 -2.0 5,866,871 + BT4456 0.58 -0.2 5,866,872 - BT4456 0.58 -1.3 5,866,908 + BT4456 0.60 -1.6 5,866,917 - BT4456 0.61 -2.5 5,866,920 - BT4456 0.61 -0.5 5,866,964 + BT4456 0.64 -0.7 5,867,007 - BT4456 0.67 -1.6 5,867,007 - BT4456 0.67 -2.1 5,867,043 + BT4456 0.70 +0.8 5,867,044 - BT4456 0.70 -2.5 5,867,044 - BT4456 0.70 -0.6 5,867,050 - BT4456 0.70 +0.6 5,867,054 + BT4456 0.71 -1.3 5,867,110 - BT4456 0.75 -1.5 5,867,139 - BT4456 0.77 -0.9 5,867,246 - BT4456 0.85 +0.4 5,867,347 - +0.3 5,867,434 + -0.2 5,867,435 - -0.9 5,867,491 + -0.5 5,867,491 + -1.0 5,867,526 + BT4457 0.12 -0.6 5,867,527 - BT4457 0.12 -0.1 5,867,527 - BT4457 0.12 -0.2 5,867,593 - BT4457 0.24 -1.8 5,867,610 + BT4457 0.26 +0.2 5,867,611 - BT4457 0.27 -2.0 5,867,694 + BT4457 0.41 -1.6 5,867,695 - BT4457 0.41 -0.8 5,867,897 + BT4457 0.76 -2.5 5,868,028 - +1.2 5,868,087 + +0.1 5,868,087 + -0.0 5,868,123 - -0.3 5,868,130 - -0.8 5,868,173 + -1.5 5,868,175 + -0.8 5,868,177 + -0.9 5,868,178 - -0.9 5,868,178 - -1.4 5,868,225 - -0.6 5,868,225 - +0.3 5,868,229 - -2.1 5,868,231 + -0.9 5,868,245 - -2.8 5,868,253 - -2.9 5,868,258 - +0.3 5,868,384 + BT4458 0.16 -1.1
Or see this region's nucleotide sequence