Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_76 and Pf6N2E2_77 are separated by 58 nucleotides Pf6N2E2_77 and Pf6N2E2_78 overlap by 4 nucleotides Pf6N2E2_78 and Pf6N2E2_6100 are separated by 168 nucleotides
Pf6N2E2_76 - Amidophosphoribosyltransferase (EC 2.4.2.14), at 117,987 to 119,492
_76
Pf6N2E2_77 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48), at 119,551 to 120,762
_77
Pf6N2E2_78 - Oxidoreductase, short chain dehydrogenase/reductase family, at 120,759 to 121,532
_78
Pf6N2E2_6100 - tRNA-Val-TAC, at 121,701 to 121,773
_6100
Position (kb)
119
120
121 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 118.680 kb on - strand, within Pf6N2E2_76 at 119.481 kb on + strand at 119.482 kb on - strand at 119.482 kb on - strand at 119.676 kb on + strand, within Pf6N2E2_77 at 119.687 kb on - strand, within Pf6N2E2_77 at 119.687 kb on - strand, within Pf6N2E2_77 at 119.687 kb on - strand, within Pf6N2E2_77 at 119.944 kb on + strand, within Pf6N2E2_77 at 119.945 kb on - strand, within Pf6N2E2_77 at 119.974 kb on + strand, within Pf6N2E2_77 at 120.024 kb on + strand, within Pf6N2E2_77 at 120.439 kb on + strand, within Pf6N2E2_77 at 121.092 kb on + strand, within Pf6N2E2_78 at 121.159 kb on - strand, within Pf6N2E2_78 at 121.236 kb on + strand, within Pf6N2E2_78 at 121.236 kb on + strand, within Pf6N2E2_78 at 121.237 kb on - strand, within Pf6N2E2_78 at 121.237 kb on - strand, within Pf6N2E2_78 at 121.412 kb on - strand, within Pf6N2E2_78 at 121.421 kb on - strand, within Pf6N2E2_78 at 121.524 kb on + strand at 121.524 kb on + strand at 121.525 kb on - strand at 121.525 kb on - strand at 121.525 kb on - strand at 121.572 kb on + strand at 121.572 kb on + strand at 121.573 kb on - strand at 121.598 kb on + strand at 121.599 kb on - strand at 121.658 kb on - strand at 121.734 kb on + strand, within Pf6N2E2_6100 at 121.734 kb on + strand, within Pf6N2E2_6100
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 118,680 - Pf6N2E2_76 0.46 +1.5 119,481 + -0.3 119,482 - -1.9 119,482 - -3.2 119,676 + Pf6N2E2_77 0.10 -1.5 119,687 - Pf6N2E2_77 0.11 -1.9 119,687 - Pf6N2E2_77 0.11 -0.8 119,687 - Pf6N2E2_77 0.11 -3.4 119,944 + Pf6N2E2_77 0.32 +0.5 119,945 - Pf6N2E2_77 0.33 -1.9 119,974 + Pf6N2E2_77 0.35 -0.8 120,024 + Pf6N2E2_77 0.39 -2.5 120,439 + Pf6N2E2_77 0.73 -2.4 121,092 + Pf6N2E2_78 0.43 -1.2 121,159 - Pf6N2E2_78 0.52 -0.2 121,236 + Pf6N2E2_78 0.62 -0.2 121,236 + Pf6N2E2_78 0.62 -0.1 121,237 - Pf6N2E2_78 0.62 +0.1 121,237 - Pf6N2E2_78 0.62 -0.3 121,412 - Pf6N2E2_78 0.84 +0.3 121,421 - Pf6N2E2_78 0.86 -0.9 121,524 + +1.3 121,524 + +0.9 121,525 - +0.5 121,525 - +0.1 121,525 - -0.5 121,572 + -0.8 121,572 + +1.1 121,573 - +1.5 121,598 + -0.1 121,599 - +1.5 121,658 - +0.4 121,734 + Pf6N2E2_6100 0.45 +0.8 121,734 + Pf6N2E2_6100 0.45 -1.8
Or see this region's nucleotide sequence