Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_737

Experiment: Nitrate reduction, replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_735 and Pf6N2E2_736 are separated by 31 nucleotidesPf6N2E2_736 and Pf6N2E2_737 are separated by 5 nucleotidesPf6N2E2_737 and Pf6N2E2_738 are separated by 110 nucleotides Pf6N2E2_735 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 843,255 to 845,270 _735 Pf6N2E2_736 - hypothetical protein, at 845,302 to 845,463 _736 Pf6N2E2_737 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 845,469 to 846,155 _737 Pf6N2E2_738 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 846,266 to 847,885 _738 Position (kb) 845 846 847Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 844.530 kb on + strand, within Pf6N2E2_735at 844.530 kb on + strand, within Pf6N2E2_735at 844.531 kb on - strand, within Pf6N2E2_735at 844.531 kb on - strand, within Pf6N2E2_735at 844.531 kb on - strand, within Pf6N2E2_735at 844.645 kb on - strand, within Pf6N2E2_735at 844.650 kb on + strand, within Pf6N2E2_735at 844.845 kb on + strand, within Pf6N2E2_735at 844.846 kb on - strand, within Pf6N2E2_735at 844.846 kb on - strand, within Pf6N2E2_735at 844.846 kb on - strand, within Pf6N2E2_735at 844.950 kb on + strand, within Pf6N2E2_735at 844.950 kb on + strand, within Pf6N2E2_735at 844.950 kb on + strand, within Pf6N2E2_735at 844.950 kb on + strand, within Pf6N2E2_735at 844.950 kb on + strand, within Pf6N2E2_735at 844.951 kb on - strand, within Pf6N2E2_735at 844.953 kb on + strand, within Pf6N2E2_735at 844.954 kb on - strand, within Pf6N2E2_735at 844.954 kb on - strand, within Pf6N2E2_735at 845.014 kb on + strand, within Pf6N2E2_735at 845.014 kb on + strand, within Pf6N2E2_735at 845.015 kb on - strand, within Pf6N2E2_735at 845.037 kb on + strand, within Pf6N2E2_735at 845.037 kb on + strand, within Pf6N2E2_735at 845.038 kb on - strand, within Pf6N2E2_735at 845.038 kb on - strand, within Pf6N2E2_735at 845.064 kb on + strand, within Pf6N2E2_735at 845.064 kb on + strand, within Pf6N2E2_735at 845.064 kb on + strand, within Pf6N2E2_735at 845.064 kb on + strand, within Pf6N2E2_735at 845.064 kb on + strand, within Pf6N2E2_735at 845.065 kb on - strand, within Pf6N2E2_735at 845.065 kb on - strand, within Pf6N2E2_735at 845.065 kb on - strand, within Pf6N2E2_735at 845.065 kb on - strand, within Pf6N2E2_735at 845.187 kb on + strandat 845.188 kb on - strandat 845.403 kb on + strand, within Pf6N2E2_736at 845.403 kb on + strand, within Pf6N2E2_736at 845.403 kb on + strand, within Pf6N2E2_736at 845.403 kb on + strand, within Pf6N2E2_736at 845.404 kb on - strand, within Pf6N2E2_736at 845.404 kb on - strand, within Pf6N2E2_736at 845.533 kb on + strandat 845.931 kb on + strand, within Pf6N2E2_737at 845.932 kb on - strand, within Pf6N2E2_737at 846.145 kb on + strandat 846.146 kb on - strandat 846.243 kb on + strandat 846.243 kb on + strandat 846.244 kb on - strandat 846.244 kb on - strandat 846.244 kb on - strandat 846.265 kb on + strandat 846.265 kb on + strandat 846.266 kb on - strandat 846.266 kb on - strandat 846.594 kb on - strand, within Pf6N2E2_738at 846.623 kb on + strand, within Pf6N2E2_738at 846.624 kb on - strand, within Pf6N2E2_738at 846.624 kb on - strand, within Pf6N2E2_738at 846.624 kb on - strand, within Pf6N2E2_738at 846.624 kb on - strand, within Pf6N2E2_738at 846.728 kb on + strand, within Pf6N2E2_738at 846.804 kb on - strand, within Pf6N2E2_738at 846.804 kb on - strand, within Pf6N2E2_738at 846.985 kb on - strand, within Pf6N2E2_738at 846.985 kb on - strand, within Pf6N2E2_738at 847.009 kb on + strand, within Pf6N2E2_738at 847.009 kb on + strand, within Pf6N2E2_738at 847.009 kb on + strand, within Pf6N2E2_738at 847.010 kb on - strand, within Pf6N2E2_738at 847.054 kb on + strand, within Pf6N2E2_738at 847.055 kb on - strand, within Pf6N2E2_738

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B
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844,530 + Pf6N2E2_735 0.63 +1.6
844,530 + Pf6N2E2_735 0.63 +1.6
844,531 - Pf6N2E2_735 0.63 -4.9
844,531 - Pf6N2E2_735 0.63 -2.5
844,531 - Pf6N2E2_735 0.63 -2.0
844,645 - Pf6N2E2_735 0.69 -2.9
844,650 + Pf6N2E2_735 0.69 -3.0
844,845 + Pf6N2E2_735 0.79 -3.2
844,846 - Pf6N2E2_735 0.79 -3.4
844,846 - Pf6N2E2_735 0.79 -1.9
844,846 - Pf6N2E2_735 0.79 -1.8
844,950 + Pf6N2E2_735 0.84 +0.3
844,950 + Pf6N2E2_735 0.84 -3.7
844,950 + Pf6N2E2_735 0.84 -1.1
844,950 + Pf6N2E2_735 0.84 -1.0
844,950 + Pf6N2E2_735 0.84 -0.4
844,951 - Pf6N2E2_735 0.84 -2.3
844,953 + Pf6N2E2_735 0.84 -2.8
844,954 - Pf6N2E2_735 0.84 -2.6
844,954 - Pf6N2E2_735 0.84 -2.9
845,014 + Pf6N2E2_735 0.87 -3.2
845,014 + Pf6N2E2_735 0.87 +1.6
845,015 - Pf6N2E2_735 0.87 -3.1
845,037 + Pf6N2E2_735 0.88 -2.4
845,037 + Pf6N2E2_735 0.88 +1.6
845,038 - Pf6N2E2_735 0.88 -3.6
845,038 - Pf6N2E2_735 0.88 -3.0
845,064 + Pf6N2E2_735 0.90 -2.5
845,064 + Pf6N2E2_735 0.90 -3.0
845,064 + Pf6N2E2_735 0.90 -1.8
845,064 + Pf6N2E2_735 0.90 -4.8
845,064 + Pf6N2E2_735 0.90 -3.6
845,065 - Pf6N2E2_735 0.90 -3.4
845,065 - Pf6N2E2_735 0.90 -1.0
845,065 - Pf6N2E2_735 0.90 -1.5
845,065 - Pf6N2E2_735 0.90 -3.0
845,187 + -4.5
845,188 - -1.8
845,403 + Pf6N2E2_736 0.62 -2.1
845,403 + Pf6N2E2_736 0.62 -3.3
845,403 + Pf6N2E2_736 0.62 -3.3
845,403 + Pf6N2E2_736 0.62 -2.0
845,404 - Pf6N2E2_736 0.63 -3.0
845,404 - Pf6N2E2_736 0.63 -1.5
845,533 + -2.3
845,931 + Pf6N2E2_737 0.67 -2.7
845,932 - Pf6N2E2_737 0.67 -2.2
846,145 + +0.2
846,146 - -1.4
846,243 + +0.3
846,243 + -0.0
846,244 - -1.0
846,244 - +1.0
846,244 - -0.4
846,265 + -0.8
846,265 + +1.2
846,266 - -0.7
846,266 - +0.2
846,594 - Pf6N2E2_738 0.20 +1.8
846,623 + Pf6N2E2_738 0.22 +0.3
846,624 - Pf6N2E2_738 0.22 +1.4
846,624 - Pf6N2E2_738 0.22 +0.4
846,624 - Pf6N2E2_738 0.22 -0.8
846,624 - Pf6N2E2_738 0.22 -0.0
846,728 + Pf6N2E2_738 0.29 -0.0
846,804 - Pf6N2E2_738 0.33 +0.6
846,804 - Pf6N2E2_738 0.33 -1.7
846,985 - Pf6N2E2_738 0.44 +0.3
846,985 - Pf6N2E2_738 0.44 +1.0
847,009 + Pf6N2E2_738 0.46 +0.3
847,009 + Pf6N2E2_738 0.46 -0.7
847,009 + Pf6N2E2_738 0.46 +2.0
847,010 - Pf6N2E2_738 0.46 +0.6
847,054 + Pf6N2E2_738 0.49 +0.2
847,055 - Pf6N2E2_738 0.49 -0.4

Or see this region's nucleotide sequence