Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_731 and Pf6N2E2_732 overlap by 8 nucleotides Pf6N2E2_732 and Pf6N2E2_733 are separated by 10 nucleotides Pf6N2E2_733 and Pf6N2E2_734 are separated by 25 nucleotides
Pf6N2E2_731 - Respiratory nitrate reductase delta chain (EC 1.7.99.4), at 839,552 to 840,307
_731
Pf6N2E2_732 - Respiratory nitrate reductase gamma chain (EC 1.7.99.4), at 840,300 to 840,980
_732
Pf6N2E2_733 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 840,991 to 841,959
_733
Pf6N2E2_734 - Molybdenum cofactor biosynthesis protein MoaA, at 841,985 to 842,977
_734
Position (kb) 840
841
842 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 840.298 kb on + strand at 840.298 kb on + strand at 840.305 kb on + strand at 840.305 kb on + strand at 840.306 kb on - strand at 840.306 kb on - strand at 840.396 kb on + strand, within Pf6N2E2_732 at 840.397 kb on - strand, within Pf6N2E2_732 at 840.397 kb on - strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.528 kb on + strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.529 kb on - strand, within Pf6N2E2_732 at 840.740 kb on + strand, within Pf6N2E2_732 at 840.852 kb on + strand, within Pf6N2E2_732 at 840.852 kb on + strand, within Pf6N2E2_732 at 840.853 kb on - strand, within Pf6N2E2_732 at 840.940 kb on - strand at 840.940 kb on - strand at 841.246 kb on + strand, within Pf6N2E2_733 at 841.247 kb on - strand, within Pf6N2E2_733 at 841.454 kb on - strand, within Pf6N2E2_733 at 841.454 kb on - strand, within Pf6N2E2_733 at 841.454 kb on - strand, within Pf6N2E2_733 at 841.882 kb on + strand at 842.073 kb on - strand at 842.517 kb on - strand, within Pf6N2E2_734 at 842.684 kb on + strand, within Pf6N2E2_734 at 842.684 kb on + strand, within Pf6N2E2_734 at 842.684 kb on + strand, within Pf6N2E2_734 at 842.685 kb on - strand, within Pf6N2E2_734
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 840,298 + -2.1 840,298 + -2.0 840,305 + -0.7 840,305 + -2.0 840,306 - -2.1 840,306 - -1.3 840,396 + Pf6N2E2_732 0.14 -3.7 840,397 - Pf6N2E2_732 0.14 -2.2 840,397 - Pf6N2E2_732 0.14 -3.4 840,528 + Pf6N2E2_732 0.33 -2.4 840,528 + Pf6N2E2_732 0.33 -2.6 840,528 + Pf6N2E2_732 0.33 -2.8 840,528 + Pf6N2E2_732 0.33 -1.9 840,528 + Pf6N2E2_732 0.33 -3.1 840,528 + Pf6N2E2_732 0.33 -3.1 840,528 + Pf6N2E2_732 0.33 -1.2 840,528 + Pf6N2E2_732 0.33 -1.4 840,529 - Pf6N2E2_732 0.34 +0.6 840,529 - Pf6N2E2_732 0.34 +1.6 840,529 - Pf6N2E2_732 0.34 -2.0 840,529 - Pf6N2E2_732 0.34 -2.7 840,529 - Pf6N2E2_732 0.34 -0.0 840,529 - Pf6N2E2_732 0.34 -2.8 840,740 + Pf6N2E2_732 0.65 -1.8 840,852 + Pf6N2E2_732 0.81 -4.1 840,852 + Pf6N2E2_732 0.81 -2.5 840,853 - Pf6N2E2_732 0.81 -1.6 840,940 - -1.0 840,940 - -2.4 841,246 + Pf6N2E2_733 0.26 -2.6 841,247 - Pf6N2E2_733 0.26 -2.5 841,454 - Pf6N2E2_733 0.48 -3.1 841,454 - Pf6N2E2_733 0.48 -0.4 841,454 - Pf6N2E2_733 0.48 -0.8 841,882 + -2.1 842,073 - +0.6 842,517 - Pf6N2E2_734 0.54 -0.1 842,684 + Pf6N2E2_734 0.70 -0.3 842,684 + Pf6N2E2_734 0.70 +0.1 842,684 + Pf6N2E2_734 0.70 -0.8 842,685 - Pf6N2E2_734 0.70 +0.1
Or see this region's nucleotide sequence