Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_656 and Pf6N2E2_657 are separated by 113 nucleotides Pf6N2E2_657 and Pf6N2E2_658 are separated by 199 nucleotides Pf6N2E2_658 and Pf6N2E2_659 overlap by 4 nucleotides Pf6N2E2_659 and Pf6N2E2_660 are separated by 223 nucleotides
Pf6N2E2_656 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 759,262 to 761,835
_656
Pf6N2E2_657 - hypothetical protein, at 761,949 to 762,065
_657
Pf6N2E2_658 - sensor histidine kinase, at 762,265 to 763,575
_658
Pf6N2E2_659 - Transcriptional regulatory protein ompR, at 763,572 to 764,312
_659
Pf6N2E2_660 - hypothetical protein, at 764,536 to 764,799
_660
Position (kb) 762
763
764 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 761.300 kb on + strand, within Pf6N2E2_656 at 761.301 kb on - strand, within Pf6N2E2_656 at 761.448 kb on - strand, within Pf6N2E2_656 at 761.448 kb on - strand, within Pf6N2E2_656 at 761.490 kb on - strand, within Pf6N2E2_656 at 761.495 kb on + strand, within Pf6N2E2_656 at 761.496 kb on - strand, within Pf6N2E2_656 at 761.504 kb on + strand, within Pf6N2E2_656 at 761.534 kb on + strand, within Pf6N2E2_656 at 761.835 kb on + strand at 761.841 kb on + strand at 761.841 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.881 kb on + strand at 761.882 kb on - strand at 761.903 kb on + strand at 761.903 kb on + strand at 761.904 kb on - strand at 761.907 kb on + strand at 761.908 kb on - strand at 762.151 kb on + strand at 762.151 kb on + strand at 762.152 kb on - strand at 762.737 kb on + strand, within Pf6N2E2_658 at 762.997 kb on - strand, within Pf6N2E2_658 at 763.144 kb on + strand, within Pf6N2E2_658 at 763.145 kb on - strand, within Pf6N2E2_658 at 763.145 kb on - strand, within Pf6N2E2_658 at 763.235 kb on + strand, within Pf6N2E2_658 at 763.328 kb on + strand, within Pf6N2E2_658 at 763.328 kb on + strand, within Pf6N2E2_658 at 763.328 kb on + strand, within Pf6N2E2_658 at 763.328 kb on + strand, within Pf6N2E2_658 at 763.328 kb on + strand, within Pf6N2E2_658 at 763.328 kb on + strand, within Pf6N2E2_658 at 763.329 kb on - strand, within Pf6N2E2_658 at 763.329 kb on - strand, within Pf6N2E2_658 at 763.329 kb on - strand, within Pf6N2E2_658 at 763.329 kb on - strand, within Pf6N2E2_658 at 763.329 kb on - strand, within Pf6N2E2_658 at 763.329 kb on - strand, within Pf6N2E2_658 at 763.332 kb on + strand, within Pf6N2E2_658 at 763.332 kb on + strand, within Pf6N2E2_658 at 763.332 kb on + strand, within Pf6N2E2_658 at 763.332 kb on + strand, within Pf6N2E2_658 at 763.332 kb on + strand, within Pf6N2E2_658 at 763.332 kb on + strand, within Pf6N2E2_658 at 763.332 kb on + strand, within Pf6N2E2_658 at 763.333 kb on - strand, within Pf6N2E2_658 at 763.333 kb on - strand, within Pf6N2E2_658 at 763.333 kb on - strand, within Pf6N2E2_658 at 763.333 kb on - strand, within Pf6N2E2_658 at 763.333 kb on - strand, within Pf6N2E2_658 at 763.333 kb on - strand, within Pf6N2E2_658 at 763.333 kb on - strand, within Pf6N2E2_658 at 763.333 kb on - strand, within Pf6N2E2_658 at 763.334 kb on + strand, within Pf6N2E2_658 at 763.334 kb on + strand, within Pf6N2E2_658 at 763.334 kb on + strand, within Pf6N2E2_658 at 763.334 kb on + strand, within Pf6N2E2_658 at 763.334 kb on + strand, within Pf6N2E2_658 at 763.334 kb on + strand, within Pf6N2E2_658 at 763.334 kb on + strand, within Pf6N2E2_658 at 763.334 kb on + strand, within Pf6N2E2_658 at 763.334 kb on + strand, within Pf6N2E2_658 at 763.335 kb on - strand, within Pf6N2E2_658 at 763.461 kb on - strand at 763.469 kb on + strand at 763.472 kb on + strand at 763.567 kb on + strand at 763.568 kb on - strand at 763.616 kb on - strand at 763.616 kb on - strand at 763.642 kb on + strand at 764.006 kb on - strand, within Pf6N2E2_659 at 764.006 kb on - strand, within Pf6N2E2_659 at 764.074 kb on - strand, within Pf6N2E2_659 at 764.246 kb on - strand at 764.286 kb on + strand at 764.287 kb on - strand at 764.287 kb on - strand at 764.349 kb on + strand at 764.384 kb on + strand at 764.385 kb on - strand at 764.385 kb on - strand at 764.385 kb on - strand at 764.385 kb on - strand at 764.394 kb on + strand at 764.395 kb on - strand at 764.510 kb on + strand at 764.511 kb on - strand at 764.554 kb on + strand at 764.555 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 761,300 + Pf6N2E2_656 0.79 -0.3 761,301 - Pf6N2E2_656 0.79 -0.7 761,448 - Pf6N2E2_656 0.85 -0.0 761,448 - Pf6N2E2_656 0.85 -0.0 761,490 - Pf6N2E2_656 0.87 +0.6 761,495 + Pf6N2E2_656 0.87 +0.3 761,496 - Pf6N2E2_656 0.87 +0.7 761,504 + Pf6N2E2_656 0.87 -0.7 761,534 + Pf6N2E2_656 0.88 +0.0 761,835 + -0.2 761,841 + -0.0 761,841 + +0.3 761,881 + -0.2 761,881 + +0.7 761,881 + +0.1 761,881 + -0.2 761,881 + -1.3 761,882 - +0.4 761,903 + -0.1 761,903 + -0.7 761,904 - +2.0 761,907 + +0.1 761,908 - +1.6 762,151 + +2.0 762,151 + -0.8 762,152 - -0.9 762,737 + Pf6N2E2_658 0.36 -0.7 762,997 - Pf6N2E2_658 0.56 -0.6 763,144 + Pf6N2E2_658 0.67 +1.6 763,145 - Pf6N2E2_658 0.67 -2.5 763,145 - Pf6N2E2_658 0.67 -1.0 763,235 + Pf6N2E2_658 0.74 -1.2 763,328 + Pf6N2E2_658 0.81 -0.5 763,328 + Pf6N2E2_658 0.81 -0.3 763,328 + Pf6N2E2_658 0.81 -0.9 763,328 + Pf6N2E2_658 0.81 -1.0 763,328 + Pf6N2E2_658 0.81 -0.2 763,328 + Pf6N2E2_658 0.81 -1.1 763,329 - Pf6N2E2_658 0.81 -0.0 763,329 - Pf6N2E2_658 0.81 +1.6 763,329 - Pf6N2E2_658 0.81 -1.0 763,329 - Pf6N2E2_658 0.81 -2.8 763,329 - Pf6N2E2_658 0.81 -1.9 763,329 - Pf6N2E2_658 0.81 -0.4 763,332 + Pf6N2E2_658 0.81 +1.6 763,332 + Pf6N2E2_658 0.81 -2.5 763,332 + Pf6N2E2_658 0.81 +1.6 763,332 + Pf6N2E2_658 0.81 +0.2 763,332 + Pf6N2E2_658 0.81 -1.9 763,332 + Pf6N2E2_658 0.81 -0.7 763,332 + Pf6N2E2_658 0.81 -1.8 763,333 - Pf6N2E2_658 0.81 -1.4 763,333 - Pf6N2E2_658 0.81 -1.2 763,333 - Pf6N2E2_658 0.81 -2.0 763,333 - Pf6N2E2_658 0.81 -0.5 763,333 - Pf6N2E2_658 0.81 +0.1 763,333 - Pf6N2E2_658 0.81 -1.4 763,333 - Pf6N2E2_658 0.81 -1.0 763,333 - Pf6N2E2_658 0.81 -1.3 763,334 + Pf6N2E2_658 0.82 -2.6 763,334 + Pf6N2E2_658 0.82 -1.0 763,334 + Pf6N2E2_658 0.82 -1.3 763,334 + Pf6N2E2_658 0.82 -0.2 763,334 + Pf6N2E2_658 0.82 -0.9 763,334 + Pf6N2E2_658 0.82 -1.8 763,334 + Pf6N2E2_658 0.82 -2.3 763,334 + Pf6N2E2_658 0.82 -1.6 763,334 + Pf6N2E2_658 0.82 -0.4 763,335 - Pf6N2E2_658 0.82 -2.4 763,461 - -1.0 763,469 + -0.4 763,472 + +0.2 763,567 + -0.5 763,568 - -0.7 763,616 - -0.1 763,616 - -0.1 763,642 + +0.7 764,006 - Pf6N2E2_659 0.59 +0.1 764,006 - Pf6N2E2_659 0.59 +1.6 764,074 - Pf6N2E2_659 0.68 +0.5 764,246 - +0.6 764,286 + +0.3 764,287 - +0.0 764,287 - -0.3 764,349 + +0.3 764,384 + +0.2 764,385 - +2.6 764,385 - +1.0 764,385 - -0.4 764,385 - -0.3 764,394 + -0.0 764,395 - -0.4 764,510 + -0.2 764,511 - +2.6 764,554 + +0.6 764,555 - -0.6
Or see this region's nucleotide sequence