Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_53 and Pf6N2E2_6096 are separated by 146 nucleotides Pf6N2E2_6096 and Pf6N2E2_6097 are separated by 82 nucleotides Pf6N2E2_6097 and Pf6N2E2_6098 are separated by 56 nucleotides Pf6N2E2_6098 and Pf6N2E2_6099 are separated by 78 nucleotides Pf6N2E2_6099 and Pf6N2E2_54 are separated by 184 nucleotides Pf6N2E2_54 and Pf6N2E2_55 are separated by 28 nucleotides
Pf6N2E2_53 - Glutamyl-tRNA synthetase (EC 6.1.1.17) @ Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24), at 92,556 to 94,037
_53
Pf6N2E2_6096 - tRNA-Ala-GGC, at 94,184 to 94,256
_6096
Pf6N2E2_6097 - tRNA-Glu-TTC, at 94,339 to 94,411
_6097
Pf6N2E2_6098 - tRNA-Ala-GGC, at 94,468 to 94,540
_6098
Pf6N2E2_6099 - tRNA-Glu-TTC, at 94,619 to 94,691
_6099
Pf6N2E2_54 - Transcriptional regulator, TetR family, at 94,876 to 95,415
_54
Pf6N2E2_55 - Probable hydrolase, at 95,444 to 96,277
_55
Position (kb)
94
95 Strain fitness (log2 ratio)
-1
0
1 at 94.263 kb on + strand at 94.263 kb on + strand at 94.263 kb on + strand at 94.324 kb on + strand at 94.546 kb on + strand at 94.546 kb on + strand at 94.663 kb on + strand, within Pf6N2E2_6099 at 94.718 kb on + strand at 94.718 kb on + strand at 94.718 kb on + strand at 95.008 kb on + strand, within Pf6N2E2_54 at 95.143 kb on + strand, within Pf6N2E2_54 at 95.143 kb on + strand, within Pf6N2E2_54 at 95.143 kb on + strand, within Pf6N2E2_54 at 95.144 kb on - strand, within Pf6N2E2_54 at 95.144 kb on - strand, within Pf6N2E2_54 at 95.253 kb on + strand, within Pf6N2E2_54 at 95.418 kb on + strand at 95.419 kb on - strand at 95.419 kb on - strand at 95.468 kb on - strand at 95.626 kb on - strand, within Pf6N2E2_55 at 95.626 kb on - strand, within Pf6N2E2_55
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 94,263 + -1.1 94,263 + +0.0 94,263 + +0.3 94,324 + +0.4 94,546 + +0.5 94,546 + -0.8 94,663 + Pf6N2E2_6099 0.60 -1.1 94,718 + -0.3 94,718 + +0.7 94,718 + -0.5 95,008 + Pf6N2E2_54 0.24 -0.4 95,143 + Pf6N2E2_54 0.49 -1.7 95,143 + Pf6N2E2_54 0.49 -0.9 95,143 + Pf6N2E2_54 0.49 +0.1 95,144 - Pf6N2E2_54 0.50 -0.8 95,144 - Pf6N2E2_54 0.50 -1.3 95,253 + Pf6N2E2_54 0.70 -0.9 95,418 + -0.3 95,419 - +0.1 95,419 - +0.5 95,468 - +1.5 95,626 - Pf6N2E2_55 0.22 -0.2 95,626 - Pf6N2E2_55 0.22 -0.6
Or see this region's nucleotide sequence