Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5726 and Pf6N2E2_5727 are separated by 276 nucleotides Pf6N2E2_5727 and Pf6N2E2_5728 are separated by 8 nucleotides Pf6N2E2_5728 and Pf6N2E2_5729 overlap by 1 nucleotides
Pf6N2E2_5726 - FIG00953102: hypothetical protein, at 6,543,812 to 6,544,006
_5726
Pf6N2E2_5727 - FIG00955360: hypothetical protein, at 6,544,283 to 6,544,996
_5727
Pf6N2E2_5728 - Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2), at 6,545,005 to 6,545,655
_5728
Pf6N2E2_5729 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 6,545,655 to 6,546,764
_5729
Position (kb)
6545
6546 Strain fitness (log2 ratio)
-2
-1
0
1 at 6544.028 kb on + strand at 6544.028 kb on + strand at 6544.028 kb on + strand at 6544.028 kb on + strand at 6544.029 kb on - strand at 6544.029 kb on - strand at 6544.062 kb on - strand at 6544.080 kb on - strand at 6544.083 kb on + strand at 6544.084 kb on - strand at 6544.084 kb on - strand at 6544.084 kb on - strand at 6544.125 kb on + strand at 6544.208 kb on + strand at 6544.208 kb on + strand at 6544.208 kb on + strand at 6544.209 kb on - strand at 6544.209 kb on - strand at 6544.209 kb on - strand at 6544.209 kb on - strand at 6544.241 kb on + strand at 6544.241 kb on + strand at 6544.242 kb on - strand at 6544.242 kb on - strand at 6544.242 kb on - strand at 6544.242 kb on - strand at 6544.338 kb on + strand at 6544.338 kb on + strand at 6544.338 kb on + strand at 6544.338 kb on + strand at 6544.338 kb on + strand at 6544.339 kb on - strand at 6544.383 kb on + strand, within Pf6N2E2_5727 at 6544.384 kb on - strand, within Pf6N2E2_5727 at 6544.518 kb on + strand, within Pf6N2E2_5727 at 6544.518 kb on + strand, within Pf6N2E2_5727 at 6544.519 kb on - strand, within Pf6N2E2_5727 at 6544.519 kb on - strand, within Pf6N2E2_5727 at 6544.519 kb on - strand, within Pf6N2E2_5727 at 6544.523 kb on - strand, within Pf6N2E2_5727 at 6544.523 kb on - strand, within Pf6N2E2_5727 at 6544.523 kb on - strand, within Pf6N2E2_5727 at 6544.551 kb on + strand, within Pf6N2E2_5727 at 6544.552 kb on - strand, within Pf6N2E2_5727 at 6544.552 kb on - strand, within Pf6N2E2_5727 at 6544.552 kb on - strand, within Pf6N2E2_5727 at 6544.602 kb on + strand, within Pf6N2E2_5727 at 6544.602 kb on + strand, within Pf6N2E2_5727 at 6544.602 kb on + strand, within Pf6N2E2_5727 at 6544.603 kb on - strand, within Pf6N2E2_5727 at 6544.603 kb on - strand, within Pf6N2E2_5727 at 6544.743 kb on + strand, within Pf6N2E2_5727 at 6544.744 kb on - strand, within Pf6N2E2_5727 at 6544.840 kb on + strand, within Pf6N2E2_5727 at 6544.911 kb on + strand, within Pf6N2E2_5727 at 6544.912 kb on - strand, within Pf6N2E2_5727 at 6544.931 kb on + strand at 6544.932 kb on - strand at 6544.932 kb on - strand at 6544.932 kb on - strand at 6544.997 kb on - strand at 6545.006 kb on + strand at 6545.007 kb on - strand at 6545.007 kb on - strand at 6545.007 kb on - strand at 6545.007 kb on - strand at 6545.115 kb on - strand, within Pf6N2E2_5728 at 6545.182 kb on + strand, within Pf6N2E2_5728 at 6545.183 kb on - strand, within Pf6N2E2_5728 at 6545.185 kb on + strand, within Pf6N2E2_5728 at 6545.290 kb on + strand, within Pf6N2E2_5728 at 6545.291 kb on - strand, within Pf6N2E2_5728 at 6545.300 kb on + strand, within Pf6N2E2_5728 at 6545.301 kb on - strand, within Pf6N2E2_5728 at 6545.301 kb on - strand, within Pf6N2E2_5728 at 6545.301 kb on - strand, within Pf6N2E2_5728 at 6545.345 kb on + strand, within Pf6N2E2_5728 at 6545.608 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 6,544,028 + +0.7 6,544,028 + -0.2 6,544,028 + -1.1 6,544,028 + -0.4 6,544,029 - -0.1 6,544,029 - +0.3 6,544,062 - -0.8 6,544,080 - +0.6 6,544,083 + +0.2 6,544,084 - -0.1 6,544,084 - -1.8 6,544,084 - -0.7 6,544,125 + -0.4 6,544,208 + -0.1 6,544,208 + -1.3 6,544,208 + -0.5 6,544,209 - +1.5 6,544,209 - -0.3 6,544,209 - +0.0 6,544,209 - +0.2 6,544,241 + -0.4 6,544,241 + -0.6 6,544,242 - -0.5 6,544,242 - +0.3 6,544,242 - +0.3 6,544,242 - -0.2 6,544,338 + +1.5 6,544,338 + -0.5 6,544,338 + +0.3 6,544,338 + +0.8 6,544,338 + +1.5 6,544,339 - +1.5 6,544,383 + Pf6N2E2_5727 0.14 -0.1 6,544,384 - Pf6N2E2_5727 0.14 -0.5 6,544,518 + Pf6N2E2_5727 0.33 -0.9 6,544,518 + Pf6N2E2_5727 0.33 -0.6 6,544,519 - Pf6N2E2_5727 0.33 +0.2 6,544,519 - Pf6N2E2_5727 0.33 +0.6 6,544,519 - Pf6N2E2_5727 0.33 +1.1 6,544,523 - Pf6N2E2_5727 0.34 +0.3 6,544,523 - Pf6N2E2_5727 0.34 +1.4 6,544,523 - Pf6N2E2_5727 0.34 +1.1 6,544,551 + Pf6N2E2_5727 0.38 +0.1 6,544,552 - Pf6N2E2_5727 0.38 +0.2 6,544,552 - Pf6N2E2_5727 0.38 -0.7 6,544,552 - Pf6N2E2_5727 0.38 -0.8 6,544,602 + Pf6N2E2_5727 0.45 +1.5 6,544,602 + Pf6N2E2_5727 0.45 +1.5 6,544,602 + Pf6N2E2_5727 0.45 +0.5 6,544,603 - Pf6N2E2_5727 0.45 -1.7 6,544,603 - Pf6N2E2_5727 0.45 +0.5 6,544,743 + Pf6N2E2_5727 0.64 +1.5 6,544,744 - Pf6N2E2_5727 0.65 +0.2 6,544,840 + Pf6N2E2_5727 0.78 +1.5 6,544,911 + Pf6N2E2_5727 0.88 -0.1 6,544,912 - Pf6N2E2_5727 0.88 +1.5 6,544,931 + -0.3 6,544,932 - +0.6 6,544,932 - +0.3 6,544,932 - +1.6 6,544,997 - +0.3 6,545,006 + +0.7 6,545,007 - -0.8 6,545,007 - -0.3 6,545,007 - -0.8 6,545,007 - -0.2 6,545,115 - Pf6N2E2_5728 0.17 -0.8 6,545,182 + Pf6N2E2_5728 0.27 +0.1 6,545,183 - Pf6N2E2_5728 0.27 -2.2 6,545,185 + Pf6N2E2_5728 0.28 -1.3 6,545,290 + Pf6N2E2_5728 0.44 -2.0 6,545,291 - Pf6N2E2_5728 0.44 -1.0 6,545,300 + Pf6N2E2_5728 0.45 -0.7 6,545,301 - Pf6N2E2_5728 0.45 -2.4 6,545,301 - Pf6N2E2_5728 0.45 -1.5 6,545,301 - Pf6N2E2_5728 0.45 -2.2 6,545,345 + Pf6N2E2_5728 0.52 -2.0 6,545,608 - -0.5
Or see this region's nucleotide sequence