Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_532 and Pf6N2E2_533 are separated by 179 nucleotides Pf6N2E2_533 and Pf6N2E2_534 overlap by 4 nucleotides Pf6N2E2_534 and Pf6N2E2_535 overlap by 4 nucleotides Pf6N2E2_535 and Pf6N2E2_536 overlap by 4 nucleotides
Pf6N2E2_532 - Na(+) H(+) antiporter subunit G, at 601,441 to 601,554
_532
Pf6N2E2_533 - Na(+) H(+) antiporter subunit G, at 601,734 to 602,087
_533
Pf6N2E2_534 - Na(+) H(+) antiporter subunit F, at 602,084 to 602,362
_534
Pf6N2E2_535 - Na(+) H(+) antiporter subunit E, at 602,359 to 602,847
_535
Pf6N2E2_536 - Na(+) H(+) antiporter subunit D, at 602,844 to 604,529
_536
Position (kb) 602
603 Strain fitness (log2 ratio) -2
-1
0
1
2 at 601.169 kb on + strand at 601.979 kb on - strand, within Pf6N2E2_533 at 601.979 kb on - strand, within Pf6N2E2_533 at 601.979 kb on - strand, within Pf6N2E2_533 at 601.979 kb on - strand, within Pf6N2E2_533 at 601.979 kb on - strand, within Pf6N2E2_533 at 602.175 kb on + strand, within Pf6N2E2_534 at 602.176 kb on - strand, within Pf6N2E2_534 at 602.176 kb on - strand, within Pf6N2E2_534 at 602.176 kb on - strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.232 kb on + strand, within Pf6N2E2_534 at 602.233 kb on - strand, within Pf6N2E2_534 at 602.233 kb on - strand, within Pf6N2E2_534 at 602.233 kb on - strand, within Pf6N2E2_534 at 602.233 kb on - strand, within Pf6N2E2_534 at 602.233 kb on - strand, within Pf6N2E2_534 at 602.239 kb on - strand, within Pf6N2E2_534 at 602.239 kb on - strand, within Pf6N2E2_534 at 602.390 kb on + strand at 602.391 kb on - strand at 602.391 kb on - strand at 602.920 kb on + strand at 602.920 kb on + strand at 602.920 kb on + strand at 602.920 kb on + strand at 602.921 kb on - strand at 602.921 kb on - strand at 602.921 kb on - strand at 602.921 kb on - strand at 602.921 kb on - strand at 603.138 kb on + strand, within Pf6N2E2_536 at 603.287 kb on + strand, within Pf6N2E2_536 at 603.288 kb on - strand, within Pf6N2E2_536 at 603.288 kb on - strand, within Pf6N2E2_536 at 603.324 kb on + strand, within Pf6N2E2_536 at 603.324 kb on + strand, within Pf6N2E2_536 at 603.325 kb on - strand, within Pf6N2E2_536 at 603.325 kb on - strand, within Pf6N2E2_536 at 603.325 kb on - strand, within Pf6N2E2_536 at 603.325 kb on - strand, within Pf6N2E2_536
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 601,169 + +0.0 601,979 - Pf6N2E2_533 0.69 -0.2 601,979 - Pf6N2E2_533 0.69 +0.1 601,979 - Pf6N2E2_533 0.69 -0.2 601,979 - Pf6N2E2_533 0.69 +2.5 601,979 - Pf6N2E2_533 0.69 +0.2 602,175 + Pf6N2E2_534 0.33 +0.3 602,176 - Pf6N2E2_534 0.33 +0.1 602,176 - Pf6N2E2_534 0.33 +0.5 602,176 - Pf6N2E2_534 0.33 +0.7 602,232 + Pf6N2E2_534 0.53 -0.0 602,232 + Pf6N2E2_534 0.53 +0.5 602,232 + Pf6N2E2_534 0.53 +0.1 602,232 + Pf6N2E2_534 0.53 -0.7 602,232 + Pf6N2E2_534 0.53 +0.5 602,232 + Pf6N2E2_534 0.53 -0.3 602,232 + Pf6N2E2_534 0.53 -0.1 602,233 - Pf6N2E2_534 0.53 -0.7 602,233 - Pf6N2E2_534 0.53 -0.5 602,233 - Pf6N2E2_534 0.53 +1.5 602,233 - Pf6N2E2_534 0.53 +0.2 602,233 - Pf6N2E2_534 0.53 +2.5 602,239 - Pf6N2E2_534 0.56 -1.8 602,239 - Pf6N2E2_534 0.56 -0.8 602,390 + -0.6 602,391 - +1.5 602,391 - -0.1 602,920 + -1.9 602,920 + +0.5 602,920 + -0.1 602,920 + +1.5 602,921 - +0.5 602,921 - -1.4 602,921 - -0.6 602,921 - +0.0 602,921 - +1.5 603,138 + Pf6N2E2_536 0.17 +0.7 603,287 + Pf6N2E2_536 0.26 -0.3 603,288 - Pf6N2E2_536 0.26 -1.8 603,288 - Pf6N2E2_536 0.26 +1.5 603,324 + Pf6N2E2_536 0.28 -0.2 603,324 + Pf6N2E2_536 0.28 -1.5 603,325 - Pf6N2E2_536 0.29 +1.5 603,325 - Pf6N2E2_536 0.29 -2.0 603,325 - Pf6N2E2_536 0.29 +1.5 603,325 - Pf6N2E2_536 0.29 +1.5
Or see this region's nucleotide sequence