Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5217 and Pf6N2E2_5218 are separated by 1 nucleotides Pf6N2E2_5218 and Pf6N2E2_5219 are separated by 4 nucleotides Pf6N2E2_5219 and Pf6N2E2_5220 are separated by 250 nucleotides
Pf6N2E2_5217 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148), at 6,015,261 to 6,016,112
_5217
Pf6N2E2_5218 - Outer membrane lipoprotein LolB precursor, at 6,016,114 to 6,016,731
_5218
Pf6N2E2_5219 - FIG140336: TPR domain protein, at 6,016,736 to 6,018,442
_5219
Pf6N2E2_5220 - Glutamyl-tRNA reductase (EC 1.2.1.70), at 6,018,693 to 6,019,922
_5220
Position (kb)
6016
6017
6018
6019 Strain fitness (log2 ratio)
-1
0
1
2
3 at 6016.738 kb on - strand at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.066 kb on - strand, within Pf6N2E2_5219 at 6017.182 kb on - strand, within Pf6N2E2_5219 at 6017.182 kb on - strand, within Pf6N2E2_5219 at 6017.182 kb on - strand, within Pf6N2E2_5219 at 6017.182 kb on - strand, within Pf6N2E2_5219 at 6017.182 kb on - strand, within Pf6N2E2_5219 at 6017.287 kb on - strand, within Pf6N2E2_5219 at 6017.404 kb on - strand, within Pf6N2E2_5219 at 6017.431 kb on - strand, within Pf6N2E2_5219 at 6018.043 kb on - strand, within Pf6N2E2_5219 at 6018.043 kb on - strand, within Pf6N2E2_5219 at 6018.043 kb on - strand, within Pf6N2E2_5219 at 6018.043 kb on - strand, within Pf6N2E2_5219 at 6018.043 kb on - strand, within Pf6N2E2_5219 at 6018.043 kb on - strand, within Pf6N2E2_5219 at 6018.043 kb on - strand, within Pf6N2E2_5219 at 6018.043 kb on - strand, within Pf6N2E2_5219 at 6018.061 kb on - strand, within Pf6N2E2_5219 at 6018.175 kb on - strand, within Pf6N2E2_5219 at 6018.241 kb on - strand, within Pf6N2E2_5219 at 6018.241 kb on - strand, within Pf6N2E2_5219 at 6018.241 kb on - strand, within Pf6N2E2_5219 at 6018.241 kb on - strand, within Pf6N2E2_5219 at 6018.241 kb on - strand, within Pf6N2E2_5219 at 6018.331 kb on - strand at 6018.455 kb on - strand at 6018.455 kb on - strand at 6018.455 kb on - strand at 6018.455 kb on - strand at 6018.455 kb on - strand at 6018.455 kb on - strand at 6018.455 kb on - strand at 6018.455 kb on - strand at 6018.461 kb on - strand at 6018.461 kb on - strand at 6018.461 kb on - strand at 6018.463 kb on - strand at 6018.463 kb on - strand at 6018.463 kb on - strand at 6018.463 kb on - strand at 6018.521 kb on + strand at 6018.538 kb on + strand at 6018.539 kb on - strand at 6018.539 kb on - strand at 6018.592 kb on + strand at 6018.592 kb on + strand at 6018.618 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 6,016,738 - +0.2 6,017,066 - Pf6N2E2_5219 0.19 +1.5 6,017,066 - Pf6N2E2_5219 0.19 +0.2 6,017,066 - Pf6N2E2_5219 0.19 +3.1 6,017,066 - Pf6N2E2_5219 0.19 +0.8 6,017,066 - Pf6N2E2_5219 0.19 +1.5 6,017,066 - Pf6N2E2_5219 0.19 +2.6 6,017,066 - Pf6N2E2_5219 0.19 +1.5 6,017,066 - Pf6N2E2_5219 0.19 +0.6 6,017,066 - Pf6N2E2_5219 0.19 +0.5 6,017,066 - Pf6N2E2_5219 0.19 +2.1 6,017,066 - Pf6N2E2_5219 0.19 -1.3 6,017,066 - Pf6N2E2_5219 0.19 +0.7 6,017,066 - Pf6N2E2_5219 0.19 -0.2 6,017,066 - Pf6N2E2_5219 0.19 -1.5 6,017,182 - Pf6N2E2_5219 0.26 -0.7 6,017,182 - Pf6N2E2_5219 0.26 +1.8 6,017,182 - Pf6N2E2_5219 0.26 +0.6 6,017,182 - Pf6N2E2_5219 0.26 +0.2 6,017,182 - Pf6N2E2_5219 0.26 +1.7 6,017,287 - Pf6N2E2_5219 0.32 -0.3 6,017,404 - Pf6N2E2_5219 0.39 +0.7 6,017,431 - Pf6N2E2_5219 0.41 +1.0 6,018,043 - Pf6N2E2_5219 0.77 +0.9 6,018,043 - Pf6N2E2_5219 0.77 +1.1 6,018,043 - Pf6N2E2_5219 0.77 +0.7 6,018,043 - Pf6N2E2_5219 0.77 +0.8 6,018,043 - Pf6N2E2_5219 0.77 +2.5 6,018,043 - Pf6N2E2_5219 0.77 +0.9 6,018,043 - Pf6N2E2_5219 0.77 +0.6 6,018,043 - Pf6N2E2_5219 0.77 +1.5 6,018,061 - Pf6N2E2_5219 0.78 +1.2 6,018,175 - Pf6N2E2_5219 0.84 +1.5 6,018,241 - Pf6N2E2_5219 0.88 -0.3 6,018,241 - Pf6N2E2_5219 0.88 +0.5 6,018,241 - Pf6N2E2_5219 0.88 +2.5 6,018,241 - Pf6N2E2_5219 0.88 +1.2 6,018,241 - Pf6N2E2_5219 0.88 +0.5 6,018,331 - +1.5 6,018,455 - +0.9 6,018,455 - +0.7 6,018,455 - +0.6 6,018,455 - +1.3 6,018,455 - -0.1 6,018,455 - +2.0 6,018,455 - +1.8 6,018,455 - +1.5 6,018,461 - +0.7 6,018,461 - +0.5 6,018,461 - +0.5 6,018,463 - +0.2 6,018,463 - +0.7 6,018,463 - +1.5 6,018,463 - +1.4 6,018,521 + +1.1 6,018,538 + +0.5 6,018,539 - -0.8 6,018,539 - +1.0 6,018,592 + +0.7 6,018,592 + +1.5 6,018,618 + -1.3
Or see this region's nucleotide sequence