Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5028 and Pf6N2E2_5029 overlap by 6 nucleotides Pf6N2E2_5029 and Pf6N2E2_5030 are separated by 235 nucleotides Pf6N2E2_5030 and Pf6N2E2_5031 are separated by 337 nucleotides
Pf6N2E2_5028 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 5,796,398 to 5,796,964
_5028
Pf6N2E2_5029 - FIG00954142: hypothetical protein, at 5,796,959 to 5,797,168
_5029
Pf6N2E2_5030 - Transcriptional regulator, AsnC family, at 5,797,404 to 5,797,883
_5030
Pf6N2E2_5031 - hypothetical protein, at 5,798,221 to 5,798,898
_5031
Position (kb) 5797
5798 Strain fitness (log2 ratio) -2
-1
0
1 at 5796.514 kb on - strand, within Pf6N2E2_5028 at 5796.524 kb on + strand, within Pf6N2E2_5028 at 5796.570 kb on + strand, within Pf6N2E2_5028 at 5796.632 kb on + strand, within Pf6N2E2_5028 at 5796.633 kb on - strand, within Pf6N2E2_5028 at 5796.633 kb on - strand, within Pf6N2E2_5028 at 5796.633 kb on - strand, within Pf6N2E2_5028 at 5796.784 kb on + strand, within Pf6N2E2_5028 at 5796.960 kb on + strand at 5796.960 kb on + strand at 5796.960 kb on + strand at 5796.960 kb on + strand at 5796.960 kb on + strand at 5796.960 kb on + strand at 5796.961 kb on - strand at 5796.961 kb on - strand at 5796.962 kb on + strand at 5796.962 kb on + strand at 5796.962 kb on + strand at 5796.963 kb on - strand at 5796.963 kb on - strand at 5797.211 kb on + strand at 5797.212 kb on - strand at 5797.213 kb on + strand at 5797.213 kb on + strand at 5797.213 kb on + strand at 5797.214 kb on - strand at 5797.562 kb on - strand, within Pf6N2E2_5030 at 5797.674 kb on + strand, within Pf6N2E2_5030 at 5797.674 kb on + strand, within Pf6N2E2_5030 at 5797.674 kb on + strand, within Pf6N2E2_5030 at 5797.675 kb on - strand, within Pf6N2E2_5030 at 5797.675 kb on - strand, within Pf6N2E2_5030 at 5797.675 kb on - strand, within Pf6N2E2_5030 at 5797.675 kb on - strand, within Pf6N2E2_5030 at 5797.675 kb on - strand, within Pf6N2E2_5030 at 5797.675 kb on - strand, within Pf6N2E2_5030 at 5797.678 kb on + strand, within Pf6N2E2_5030 at 5797.679 kb on - strand, within Pf6N2E2_5030 at 5797.738 kb on + strand, within Pf6N2E2_5030 at 5797.739 kb on - strand, within Pf6N2E2_5030 at 5797.739 kb on - strand, within Pf6N2E2_5030 at 5797.919 kb on - strand at 5797.922 kb on + strand at 5798.002 kb on + strand at 5798.002 kb on + strand at 5798.003 kb on - strand at 5798.142 kb on + strand at 5798.143 kb on - strand at 5798.143 kb on - strand at 5798.197 kb on + strand at 5798.197 kb on + strand at 5798.198 kb on - strand at 5798.198 kb on - strand at 5798.435 kb on + strand, within Pf6N2E2_5031 at 5798.436 kb on - strand, within Pf6N2E2_5031 at 5798.519 kb on + strand, within Pf6N2E2_5031 at 5798.520 kb on - strand, within Pf6N2E2_5031 at 5798.637 kb on - strand, within Pf6N2E2_5031 at 5798.637 kb on - strand, within Pf6N2E2_5031 at 5798.684 kb on + strand, within Pf6N2E2_5031 at 5798.684 kb on + strand, within Pf6N2E2_5031 at 5798.685 kb on - strand, within Pf6N2E2_5031 at 5798.695 kb on + strand, within Pf6N2E2_5031 at 5798.695 kb on + strand, within Pf6N2E2_5031 at 5798.696 kb on - strand, within Pf6N2E2_5031 at 5798.814 kb on - strand, within Pf6N2E2_5031 at 5798.814 kb on - strand, within Pf6N2E2_5031 at 5798.817 kb on + strand, within Pf6N2E2_5031 at 5798.818 kb on - strand, within Pf6N2E2_5031 at 5798.873 kb on + strand at 5798.874 kb on - strand at 5798.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 5,796,514 - Pf6N2E2_5028 0.20 -0.1 5,796,524 + Pf6N2E2_5028 0.22 -0.1 5,796,570 + Pf6N2E2_5028 0.30 +0.3 5,796,632 + Pf6N2E2_5028 0.41 +0.4 5,796,633 - Pf6N2E2_5028 0.41 -0.3 5,796,633 - Pf6N2E2_5028 0.41 -1.0 5,796,633 - Pf6N2E2_5028 0.41 -2.0 5,796,784 + Pf6N2E2_5028 0.68 +0.6 5,796,960 + -0.7 5,796,960 + +0.5 5,796,960 + -0.2 5,796,960 + +1.5 5,796,960 + -0.1 5,796,960 + -0.3 5,796,961 - +0.9 5,796,961 - -0.1 5,796,962 + +0.0 5,796,962 + +1.6 5,796,962 + +0.2 5,796,963 - -0.8 5,796,963 - +0.3 5,797,211 + +0.3 5,797,212 - +0.9 5,797,213 + +0.3 5,797,213 + +0.6 5,797,213 + +0.5 5,797,214 - +0.8 5,797,562 - Pf6N2E2_5030 0.33 -1.6 5,797,674 + Pf6N2E2_5030 0.56 -1.2 5,797,674 + Pf6N2E2_5030 0.56 -1.2 5,797,674 + Pf6N2E2_5030 0.56 -1.5 5,797,675 - Pf6N2E2_5030 0.56 -2.0 5,797,675 - Pf6N2E2_5030 0.56 -1.6 5,797,675 - Pf6N2E2_5030 0.56 +0.9 5,797,675 - Pf6N2E2_5030 0.56 +0.0 5,797,675 - Pf6N2E2_5030 0.56 -1.3 5,797,675 - Pf6N2E2_5030 0.56 -1.5 5,797,678 + Pf6N2E2_5030 0.57 -0.5 5,797,679 - Pf6N2E2_5030 0.57 -1.0 5,797,738 + Pf6N2E2_5030 0.70 -0.6 5,797,739 - Pf6N2E2_5030 0.70 -2.0 5,797,739 - Pf6N2E2_5030 0.70 -1.0 5,797,919 - +0.5 5,797,922 + +0.3 5,798,002 + -0.0 5,798,002 + +0.6 5,798,003 - +0.7 5,798,142 + +0.3 5,798,143 - -0.3 5,798,143 - -1.7 5,798,197 + +0.2 5,798,197 + +0.7 5,798,198 - +0.0 5,798,198 - +0.3 5,798,435 + Pf6N2E2_5031 0.32 -1.1 5,798,436 - Pf6N2E2_5031 0.32 +0.5 5,798,519 + Pf6N2E2_5031 0.44 -0.3 5,798,520 - Pf6N2E2_5031 0.44 +0.1 5,798,637 - Pf6N2E2_5031 0.61 -0.9 5,798,637 - Pf6N2E2_5031 0.61 +0.2 5,798,684 + Pf6N2E2_5031 0.68 +1.0 5,798,684 + Pf6N2E2_5031 0.68 +0.3 5,798,685 - Pf6N2E2_5031 0.68 -0.2 5,798,695 + Pf6N2E2_5031 0.70 -0.4 5,798,695 + Pf6N2E2_5031 0.70 -0.3 5,798,696 - Pf6N2E2_5031 0.70 -0.1 5,798,814 - Pf6N2E2_5031 0.87 +0.6 5,798,814 - Pf6N2E2_5031 0.87 +0.7 5,798,817 + Pf6N2E2_5031 0.88 -1.3 5,798,818 - Pf6N2E2_5031 0.88 -0.3 5,798,873 + -0.2 5,798,874 - +0.5 5,798,874 - -0.5
Or see this region's nucleotide sequence