Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4926 and Pf6N2E2_4927 are separated by 110 nucleotides Pf6N2E2_4927 and Pf6N2E2_4928 overlap by 4 nucleotides Pf6N2E2_4928 and Pf6N2E2_4929 overlap by 1 nucleotides Pf6N2E2_4929 and Pf6N2E2_4930 are separated by 65 nucleotides
Pf6N2E2_4926 - hypothetical protein, at 5,679,096 to 5,679,806
_4926
Pf6N2E2_4927 - GTP cyclohydrolase II (EC 3.5.4.25), at 5,679,917 to 5,680,534
_4927
Pf6N2E2_4928 - Permeases of the major facilitator superfamily, at 5,680,531 to 5,680,950
_4928
Pf6N2E2_4929 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 5,680,950 to 5,681,735
_4929
Pf6N2E2_4930 - Outer membrane vitamin B12 receptor BtuB, at 5,681,801 to 5,683,681
_4930
Position (kb) 5680
5681 Strain fitness (log2 ratio) -1
0
1 at 5679.555 kb on + strand, within Pf6N2E2_4926 at 5679.556 kb on - strand, within Pf6N2E2_4926 at 5679.648 kb on + strand, within Pf6N2E2_4926 at 5679.680 kb on + strand, within Pf6N2E2_4926 at 5679.680 kb on + strand, within Pf6N2E2_4926 at 5679.680 kb on + strand, within Pf6N2E2_4926 at 5679.681 kb on - strand, within Pf6N2E2_4926 at 5679.768 kb on + strand at 5679.769 kb on - strand at 5679.814 kb on + strand at 5679.827 kb on + strand at 5679.843 kb on + strand at 5679.843 kb on + strand at 5679.867 kb on + strand at 5679.867 kb on + strand at 5679.867 kb on + strand at 5679.867 kb on + strand at 5679.867 kb on + strand at 5679.867 kb on + strand at 5679.895 kb on + strand at 5679.895 kb on + strand at 5679.898 kb on + strand at 5679.898 kb on + strand at 5679.898 kb on + strand at 5680.579 kb on + strand, within Pf6N2E2_4928 at 5680.580 kb on - strand, within Pf6N2E2_4928 at 5680.918 kb on + strand at 5680.918 kb on + strand at 5680.918 kb on + strand at 5680.918 kb on + strand at 5680.918 kb on + strand at 5680.918 kb on + strand at 5680.919 kb on - strand at 5680.919 kb on - strand at 5680.919 kb on - strand at 5680.919 kb on - strand at 5680.919 kb on - strand at 5681.122 kb on + strand, within Pf6N2E2_4929 at 5681.123 kb on - strand, within Pf6N2E2_4929 at 5681.257 kb on - strand, within Pf6N2E2_4929 at 5681.257 kb on - strand, within Pf6N2E2_4929 at 5681.257 kb on - strand, within Pf6N2E2_4929 at 5681.421 kb on + strand, within Pf6N2E2_4929 at 5681.421 kb on + strand, within Pf6N2E2_4929 at 5681.421 kb on + strand, within Pf6N2E2_4929 at 5681.421 kb on + strand, within Pf6N2E2_4929 at 5681.421 kb on + strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.422 kb on - strand, within Pf6N2E2_4929 at 5681.445 kb on + strand, within Pf6N2E2_4929 at 5681.446 kb on - strand, within Pf6N2E2_4929 at 5681.446 kb on - strand, within Pf6N2E2_4929 at 5681.707 kb on + strand at 5681.707 kb on + strand at 5681.707 kb on + strand at 5681.707 kb on + strand at 5681.708 kb on - strand at 5681.734 kb on - strand at 5681.881 kb on + strand at 5681.881 kb on + strand at 5681.883 kb on + strand at 5681.883 kb on + strand at 5681.883 kb on + strand at 5681.884 kb on - strand at 5681.884 kb on - strand at 5681.884 kb on - strand at 5681.884 kb on - strand at 5681.898 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 5,679,555 + Pf6N2E2_4926 0.65 +1.5 5,679,556 - Pf6N2E2_4926 0.65 +0.3 5,679,648 + Pf6N2E2_4926 0.78 +0.5 5,679,680 + Pf6N2E2_4926 0.82 -1.2 5,679,680 + Pf6N2E2_4926 0.82 +0.9 5,679,680 + Pf6N2E2_4926 0.82 -0.2 5,679,681 - Pf6N2E2_4926 0.82 -0.0 5,679,768 + +0.3 5,679,769 - +0.5 5,679,814 + +1.5 5,679,827 + +0.3 5,679,843 + +1.5 5,679,843 + +0.1 5,679,867 + -0.5 5,679,867 + +0.5 5,679,867 + +0.9 5,679,867 + +0.6 5,679,867 + +0.1 5,679,867 + +0.4 5,679,895 + +1.0 5,679,895 + +0.2 5,679,898 + -0.2 5,679,898 + +1.5 5,679,898 + +0.4 5,680,579 + Pf6N2E2_4928 0.11 -1.5 5,680,580 - Pf6N2E2_4928 0.12 +1.5 5,680,918 + -0.5 5,680,918 + -0.3 5,680,918 + +1.5 5,680,918 + -0.1 5,680,918 + -0.4 5,680,918 + +0.3 5,680,919 - +1.2 5,680,919 - +1.1 5,680,919 - +0.4 5,680,919 - -0.1 5,680,919 - -0.8 5,681,122 + Pf6N2E2_4929 0.22 -0.3 5,681,123 - Pf6N2E2_4929 0.22 -0.4 5,681,257 - Pf6N2E2_4929 0.39 +0.0 5,681,257 - Pf6N2E2_4929 0.39 +0.1 5,681,257 - Pf6N2E2_4929 0.39 -0.2 5,681,421 + Pf6N2E2_4929 0.60 -1.2 5,681,421 + Pf6N2E2_4929 0.60 -1.1 5,681,421 + Pf6N2E2_4929 0.60 +1.5 5,681,421 + Pf6N2E2_4929 0.60 +0.3 5,681,421 + Pf6N2E2_4929 0.60 -0.8 5,681,422 - Pf6N2E2_4929 0.60 -1.4 5,681,422 - Pf6N2E2_4929 0.60 -0.8 5,681,422 - Pf6N2E2_4929 0.60 +0.5 5,681,422 - Pf6N2E2_4929 0.60 +0.2 5,681,422 - Pf6N2E2_4929 0.60 -0.7 5,681,422 - Pf6N2E2_4929 0.60 -0.2 5,681,445 + Pf6N2E2_4929 0.63 -0.4 5,681,446 - Pf6N2E2_4929 0.63 +0.5 5,681,446 - Pf6N2E2_4929 0.63 +0.8 5,681,707 + -0.2 5,681,707 + -0.1 5,681,707 + +0.4 5,681,707 + -0.5 5,681,708 - +0.3 5,681,734 - -1.0 5,681,881 + -0.9 5,681,881 + +1.3 5,681,883 + -1.3 5,681,883 + -0.7 5,681,883 + -1.1 5,681,884 - -0.6 5,681,884 - -1.2 5,681,884 - -0.9 5,681,884 - +0.9 5,681,898 + +0.7
Or see this region's nucleotide sequence