Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4825 and Pf6N2E2_4826 overlap by 4 nucleotides Pf6N2E2_4826 and Pf6N2E2_4827 are separated by 3 nucleotides Pf6N2E2_4827 and Pf6N2E2_4828 overlap by 4 nucleotides
Pf6N2E2_4825 - Anthranilate phosphoribosyltransferase (EC 2.4.2.18), at 5,579,815 to 5,580,864
_4825
Pf6N2E2_4826 - Indole-3-glycerol phosphate synthase (EC 4.1.1.48), at 5,580,861 to 5,581,697
_4826
Pf6N2E2_4827 - Lipoate-protein ligase A, at 5,581,701 to 5,582,402
_4827
Pf6N2E2_4828 - Vfr transcriptional regulator / Cyclic AMP receptor protein, at 5,582,399 to 5,583,043
_4828
Position (kb)
5580
5581
5582 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5580.081 kb on + strand, within Pf6N2E2_4825 at 5580.081 kb on + strand, within Pf6N2E2_4825 at 5580.208 kb on + strand, within Pf6N2E2_4825 at 5580.208 kb on + strand, within Pf6N2E2_4825 at 5580.208 kb on + strand, within Pf6N2E2_4825 at 5580.208 kb on + strand, within Pf6N2E2_4825 at 5580.209 kb on - strand, within Pf6N2E2_4825 at 5580.209 kb on - strand, within Pf6N2E2_4825 at 5580.243 kb on + strand, within Pf6N2E2_4825 at 5580.288 kb on + strand, within Pf6N2E2_4825 at 5580.363 kb on + strand, within Pf6N2E2_4825 at 5580.363 kb on + strand, within Pf6N2E2_4825 at 5580.363 kb on + strand, within Pf6N2E2_4825 at 5580.392 kb on + strand, within Pf6N2E2_4825 at 5580.393 kb on - strand, within Pf6N2E2_4825 at 5580.516 kb on + strand, within Pf6N2E2_4825 at 5580.517 kb on - strand, within Pf6N2E2_4825 at 5580.517 kb on - strand, within Pf6N2E2_4825 at 5580.517 kb on - strand, within Pf6N2E2_4825 at 5580.517 kb on - strand, within Pf6N2E2_4825 at 5580.556 kb on + strand, within Pf6N2E2_4825 at 5580.582 kb on + strand, within Pf6N2E2_4825 at 5580.728 kb on - strand, within Pf6N2E2_4825 at 5580.728 kb on - strand, within Pf6N2E2_4825 at 5580.831 kb on + strand at 5580.831 kb on + strand at 5580.831 kb on + strand at 5580.831 kb on + strand at 5580.831 kb on + strand at 5580.832 kb on - strand at 5580.832 kb on - strand at 5580.839 kb on + strand at 5580.839 kb on + strand at 5580.840 kb on - strand at 5580.966 kb on - strand, within Pf6N2E2_4826 at 5581.113 kb on + strand, within Pf6N2E2_4826 at 5581.130 kb on + strand, within Pf6N2E2_4826 at 5581.130 kb on + strand, within Pf6N2E2_4826 at 5581.130 kb on + strand, within Pf6N2E2_4826 at 5581.182 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.584 kb on + strand, within Pf6N2E2_4826 at 5581.867 kb on - strand, within Pf6N2E2_4827 at 5581.879 kb on + strand, within Pf6N2E2_4827 at 5581.959 kb on - strand, within Pf6N2E2_4827 at 5581.971 kb on + strand, within Pf6N2E2_4827 at 5581.971 kb on + strand, within Pf6N2E2_4827 at 5581.972 kb on - strand, within Pf6N2E2_4827 at 5581.972 kb on - strand, within Pf6N2E2_4827 at 5581.972 kb on - strand, within Pf6N2E2_4827 at 5582.006 kb on + strand, within Pf6N2E2_4827 at 5582.367 kb on + strand at 5582.650 kb on + strand, within Pf6N2E2_4828 at 5582.650 kb on + strand, within Pf6N2E2_4828 at 5582.651 kb on - strand, within Pf6N2E2_4828 at 5582.651 kb on - strand, within Pf6N2E2_4828 at 5582.651 kb on - strand, within Pf6N2E2_4828 at 5582.685 kb on - strand, within Pf6N2E2_4828 at 5582.685 kb on - strand, within Pf6N2E2_4828 at 5582.685 kb on - strand, within Pf6N2E2_4828 at 5582.685 kb on - strand, within Pf6N2E2_4828 at 5582.688 kb on + strand, within Pf6N2E2_4828 at 5582.688 kb on + strand, within Pf6N2E2_4828 at 5582.689 kb on - strand, within Pf6N2E2_4828 at 5582.689 kb on - strand, within Pf6N2E2_4828
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 5,580,081 + Pf6N2E2_4825 0.25 -3.4 5,580,081 + Pf6N2E2_4825 0.25 -3.2 5,580,208 + Pf6N2E2_4825 0.37 -1.9 5,580,208 + Pf6N2E2_4825 0.37 -2.5 5,580,208 + Pf6N2E2_4825 0.37 +0.5 5,580,208 + Pf6N2E2_4825 0.37 -1.7 5,580,209 - Pf6N2E2_4825 0.38 -1.5 5,580,209 - Pf6N2E2_4825 0.38 -2.8 5,580,243 + Pf6N2E2_4825 0.41 -2.1 5,580,288 + Pf6N2E2_4825 0.45 -0.1 5,580,363 + Pf6N2E2_4825 0.52 -3.8 5,580,363 + Pf6N2E2_4825 0.52 +1.5 5,580,363 + Pf6N2E2_4825 0.52 -2.8 5,580,392 + Pf6N2E2_4825 0.55 -2.6 5,580,393 - Pf6N2E2_4825 0.55 -1.3 5,580,516 + Pf6N2E2_4825 0.67 -2.0 5,580,517 - Pf6N2E2_4825 0.67 -2.2 5,580,517 - Pf6N2E2_4825 0.67 -3.3 5,580,517 - Pf6N2E2_4825 0.67 -1.7 5,580,517 - Pf6N2E2_4825 0.67 -2.4 5,580,556 + Pf6N2E2_4825 0.71 -3.1 5,580,582 + Pf6N2E2_4825 0.73 -3.2 5,580,728 - Pf6N2E2_4825 0.87 -3.0 5,580,728 - Pf6N2E2_4825 0.87 -2.7 5,580,831 + -1.6 5,580,831 + -3.2 5,580,831 + -1.8 5,580,831 + -2.5 5,580,831 + -2.6 5,580,832 - -3.7 5,580,832 - -3.5 5,580,839 + -0.3 5,580,839 + -0.6 5,580,840 - -2.8 5,580,966 - Pf6N2E2_4826 0.13 -1.1 5,581,113 + Pf6N2E2_4826 0.30 -3.7 5,581,130 + Pf6N2E2_4826 0.32 -2.0 5,581,130 + Pf6N2E2_4826 0.32 -3.2 5,581,130 + Pf6N2E2_4826 0.32 -2.6 5,581,182 + Pf6N2E2_4826 0.38 -2.3 5,581,584 + Pf6N2E2_4826 0.86 -3.2 5,581,584 + Pf6N2E2_4826 0.86 -0.5 5,581,584 + Pf6N2E2_4826 0.86 -1.8 5,581,584 + Pf6N2E2_4826 0.86 -3.4 5,581,584 + Pf6N2E2_4826 0.86 -4.2 5,581,584 + Pf6N2E2_4826 0.86 -1.8 5,581,584 + Pf6N2E2_4826 0.86 +1.5 5,581,584 + Pf6N2E2_4826 0.86 -3.3 5,581,584 + Pf6N2E2_4826 0.86 -2.5 5,581,867 - Pf6N2E2_4827 0.24 +0.0 5,581,879 + Pf6N2E2_4827 0.25 +0.1 5,581,959 - Pf6N2E2_4827 0.37 -0.2 5,581,971 + Pf6N2E2_4827 0.38 -0.1 5,581,971 + Pf6N2E2_4827 0.38 -0.1 5,581,972 - Pf6N2E2_4827 0.39 +0.1 5,581,972 - Pf6N2E2_4827 0.39 -0.9 5,581,972 - Pf6N2E2_4827 0.39 -0.1 5,582,006 + Pf6N2E2_4827 0.43 -0.6 5,582,367 + -0.2 5,582,650 + Pf6N2E2_4828 0.39 +0.6 5,582,650 + Pf6N2E2_4828 0.39 -0.7 5,582,651 - Pf6N2E2_4828 0.39 -0.4 5,582,651 - Pf6N2E2_4828 0.39 -0.6 5,582,651 - Pf6N2E2_4828 0.39 -0.6 5,582,685 - Pf6N2E2_4828 0.44 +0.6 5,582,685 - Pf6N2E2_4828 0.44 +0.7 5,582,685 - Pf6N2E2_4828 0.44 +1.1 5,582,685 - Pf6N2E2_4828 0.44 +0.5 5,582,688 + Pf6N2E2_4828 0.45 -0.1 5,582,688 + Pf6N2E2_4828 0.45 -0.2 5,582,689 - Pf6N2E2_4828 0.45 -0.6 5,582,689 - Pf6N2E2_4828 0.45 -1.0
Or see this region's nucleotide sequence