Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4578 and Pf6N2E2_4579 are separated by 28 nucleotides Pf6N2E2_4579 and Pf6N2E2_4580 overlap by 1 nucleotides
Pf6N2E2_4578 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 5,308,472 to 5,309,473
_4578
Pf6N2E2_4579 - Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-), at 5,309,502 to 5,310,113
_4579
Pf6N2E2_4580 - FIG015287: Zinc protease, at 5,310,113 to 5,311,603
_4580
Position (kb)
5309
5310
5311 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5308.521 kb on + strand at 5308.521 kb on + strand at 5308.521 kb on + strand at 5308.524 kb on + strand at 5308.524 kb on + strand at 5308.604 kb on + strand, within Pf6N2E2_4578 at 5308.719 kb on + strand, within Pf6N2E2_4578 at 5308.723 kb on - strand, within Pf6N2E2_4578 at 5308.878 kb on + strand, within Pf6N2E2_4578 at 5308.878 kb on + strand, within Pf6N2E2_4578 at 5308.878 kb on + strand, within Pf6N2E2_4578 at 5308.878 kb on + strand, within Pf6N2E2_4578 at 5308.879 kb on - strand, within Pf6N2E2_4578 at 5308.879 kb on - strand, within Pf6N2E2_4578 at 5308.879 kb on - strand, within Pf6N2E2_4578 at 5308.879 kb on - strand, within Pf6N2E2_4578 at 5308.879 kb on - strand, within Pf6N2E2_4578 at 5308.879 kb on - strand, within Pf6N2E2_4578 at 5308.879 kb on - strand, within Pf6N2E2_4578 at 5308.879 kb on - strand, within Pf6N2E2_4578 at 5308.879 kb on - strand, within Pf6N2E2_4578 at 5308.879 kb on - strand, within Pf6N2E2_4578 at 5308.879 kb on - strand, within Pf6N2E2_4578 at 5308.911 kb on + strand, within Pf6N2E2_4578 at 5308.912 kb on - strand, within Pf6N2E2_4578 at 5309.139 kb on + strand, within Pf6N2E2_4578 at 5309.140 kb on - strand, within Pf6N2E2_4578 at 5309.140 kb on - strand, within Pf6N2E2_4578 at 5309.241 kb on + strand, within Pf6N2E2_4578 at 5309.241 kb on + strand, within Pf6N2E2_4578 at 5309.241 kb on + strand, within Pf6N2E2_4578 at 5309.242 kb on - strand, within Pf6N2E2_4578 at 5309.474 kb on + strand at 5309.474 kb on + strand at 5309.474 kb on + strand at 5309.474 kb on + strand at 5309.474 kb on + strand at 5309.475 kb on - strand at 5309.475 kb on - strand at 5309.475 kb on - strand at 5309.540 kb on - strand at 5309.540 kb on - strand at 5309.540 kb on - strand at 5309.542 kb on + strand at 5309.542 kb on + strand at 5309.543 kb on - strand at 5309.543 kb on - strand at 5309.543 kb on - strand at 5309.626 kb on + strand, within Pf6N2E2_4579 at 5309.626 kb on + strand, within Pf6N2E2_4579 at 5309.627 kb on - strand, within Pf6N2E2_4579 at 5309.945 kb on - strand, within Pf6N2E2_4579 at 5310.053 kb on + strand at 5310.074 kb on + strand at 5310.115 kb on - strand at 5310.115 kb on - strand at 5310.297 kb on + strand, within Pf6N2E2_4580 at 5310.298 kb on - strand, within Pf6N2E2_4580 at 5310.315 kb on + strand, within Pf6N2E2_4580 at 5310.315 kb on + strand, within Pf6N2E2_4580 at 5310.315 kb on + strand, within Pf6N2E2_4580 at 5310.315 kb on + strand, within Pf6N2E2_4580 at 5310.316 kb on - strand, within Pf6N2E2_4580 at 5310.316 kb on - strand, within Pf6N2E2_4580 at 5310.316 kb on - strand, within Pf6N2E2_4580 at 5310.438 kb on + strand, within Pf6N2E2_4580 at 5310.439 kb on - strand, within Pf6N2E2_4580 at 5310.472 kb on + strand, within Pf6N2E2_4580 at 5310.473 kb on - strand, within Pf6N2E2_4580 at 5310.473 kb on - strand, within Pf6N2E2_4580 at 5310.473 kb on - strand, within Pf6N2E2_4580 at 5310.538 kb on + strand, within Pf6N2E2_4580 at 5310.539 kb on - strand, within Pf6N2E2_4580 at 5310.550 kb on - strand, within Pf6N2E2_4580 at 5310.550 kb on - strand, within Pf6N2E2_4580 at 5310.550 kb on - strand, within Pf6N2E2_4580 at 5310.550 kb on - strand, within Pf6N2E2_4580 at 5310.558 kb on + strand, within Pf6N2E2_4580 at 5310.651 kb on + strand, within Pf6N2E2_4580 at 5310.954 kb on + strand, within Pf6N2E2_4580 at 5310.954 kb on + strand, within Pf6N2E2_4580 at 5310.954 kb on + strand, within Pf6N2E2_4580 at 5310.954 kb on + strand, within Pf6N2E2_4580 at 5310.954 kb on + strand, within Pf6N2E2_4580 at 5310.955 kb on - strand, within Pf6N2E2_4580 at 5310.970 kb on - strand, within Pf6N2E2_4580
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 5,308,521 + +1.4 5,308,521 + +0.7 5,308,521 + -0.0 5,308,524 + -1.6 5,308,524 + +0.3 5,308,604 + Pf6N2E2_4578 0.13 -0.1 5,308,719 + Pf6N2E2_4578 0.25 -0.8 5,308,723 - Pf6N2E2_4578 0.25 +0.0 5,308,878 + Pf6N2E2_4578 0.41 +0.2 5,308,878 + Pf6N2E2_4578 0.41 +0.2 5,308,878 + Pf6N2E2_4578 0.41 +0.9 5,308,878 + Pf6N2E2_4578 0.41 -1.1 5,308,879 - Pf6N2E2_4578 0.41 -0.7 5,308,879 - Pf6N2E2_4578 0.41 -0.6 5,308,879 - Pf6N2E2_4578 0.41 -0.5 5,308,879 - Pf6N2E2_4578 0.41 +1.2 5,308,879 - Pf6N2E2_4578 0.41 +0.3 5,308,879 - Pf6N2E2_4578 0.41 +0.1 5,308,879 - Pf6N2E2_4578 0.41 +1.4 5,308,879 - Pf6N2E2_4578 0.41 -0.3 5,308,879 - Pf6N2E2_4578 0.41 +0.8 5,308,879 - Pf6N2E2_4578 0.41 -0.3 5,308,879 - Pf6N2E2_4578 0.41 -0.4 5,308,911 + Pf6N2E2_4578 0.44 -1.2 5,308,912 - Pf6N2E2_4578 0.44 -0.9 5,309,139 + Pf6N2E2_4578 0.67 -0.8 5,309,140 - Pf6N2E2_4578 0.67 +1.9 5,309,140 - Pf6N2E2_4578 0.67 +1.4 5,309,241 + Pf6N2E2_4578 0.77 -0.2 5,309,241 + Pf6N2E2_4578 0.77 -0.4 5,309,241 + Pf6N2E2_4578 0.77 -0.3 5,309,242 - Pf6N2E2_4578 0.77 +0.2 5,309,474 + -0.0 5,309,474 + -0.8 5,309,474 + -0.4 5,309,474 + +1.0 5,309,474 + -0.5 5,309,475 - -0.2 5,309,475 - +1.4 5,309,475 - -0.7 5,309,540 - +1.4 5,309,540 - +0.4 5,309,540 - +0.2 5,309,542 + +0.0 5,309,542 + -2.0 5,309,543 - -0.6 5,309,543 - +1.4 5,309,543 - +1.4 5,309,626 + Pf6N2E2_4579 0.20 +0.4 5,309,626 + Pf6N2E2_4579 0.20 -1.4 5,309,627 - Pf6N2E2_4579 0.20 +0.4 5,309,945 - Pf6N2E2_4579 0.72 +1.4 5,310,053 + +1.4 5,310,074 + +1.4 5,310,115 - -0.2 5,310,115 - -0.7 5,310,297 + Pf6N2E2_4580 0.12 -0.1 5,310,298 - Pf6N2E2_4580 0.12 -0.0 5,310,315 + Pf6N2E2_4580 0.14 +1.4 5,310,315 + Pf6N2E2_4580 0.14 +0.4 5,310,315 + Pf6N2E2_4580 0.14 +1.4 5,310,315 + Pf6N2E2_4580 0.14 +0.1 5,310,316 - Pf6N2E2_4580 0.14 -1.1 5,310,316 - Pf6N2E2_4580 0.14 -0.2 5,310,316 - Pf6N2E2_4580 0.14 -0.5 5,310,438 + Pf6N2E2_4580 0.22 +1.4 5,310,439 - Pf6N2E2_4580 0.22 +0.2 5,310,472 + Pf6N2E2_4580 0.24 +1.4 5,310,473 - Pf6N2E2_4580 0.24 +0.3 5,310,473 - Pf6N2E2_4580 0.24 -1.4 5,310,473 - Pf6N2E2_4580 0.24 +0.1 5,310,538 + Pf6N2E2_4580 0.29 +1.4 5,310,539 - Pf6N2E2_4580 0.29 +0.0 5,310,550 - Pf6N2E2_4580 0.29 -1.2 5,310,550 - Pf6N2E2_4580 0.29 +0.2 5,310,550 - Pf6N2E2_4580 0.29 +0.7 5,310,550 - Pf6N2E2_4580 0.29 -0.4 5,310,558 + Pf6N2E2_4580 0.30 +0.4 5,310,651 + Pf6N2E2_4580 0.36 +1.4 5,310,954 + Pf6N2E2_4580 0.56 +1.4 5,310,954 + Pf6N2E2_4580 0.56 +1.4 5,310,954 + Pf6N2E2_4580 0.56 +0.4 5,310,954 + Pf6N2E2_4580 0.56 +1.4 5,310,954 + Pf6N2E2_4580 0.56 -0.6 5,310,955 - Pf6N2E2_4580 0.56 -1.1 5,310,970 - Pf6N2E2_4580 0.57 +0.2
Or see this region's nucleotide sequence