Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_38 and Pf6N2E2_39 are separated by 143 nucleotides Pf6N2E2_39 and Pf6N2E2_40 overlap by 1 nucleotides Pf6N2E2_40 and Pf6N2E2_41 overlap by 1 nucleotides Pf6N2E2_41 and Pf6N2E2_42 overlap by 4 nucleotides
Pf6N2E2_38 - FIG00954456: hypothetical protein, at 77,278 to 78,357
_38
Pf6N2E2_39 - FIG00857858: hypothetical protein, at 78,501 to 78,809
_39
Pf6N2E2_40 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 78,809 to 79,120
_40
Pf6N2E2_41 - Two-component system response regulator QseB, at 79,120 to 79,788
_41
Pf6N2E2_42 - Sensor protein PhoQ (EC 2.7.13.3), at 79,785 to 81,098
_42
Position (kb) 78
79
80 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.065 kb on + strand, within Pf6N2E2_38 at 78.066 kb on - strand, within Pf6N2E2_38 at 78.066 kb on - strand, within Pf6N2E2_38 at 78.066 kb on - strand, within Pf6N2E2_38 at 78.388 kb on + strand at 78.389 kb on - strand at 78.389 kb on - strand at 78.389 kb on - strand at 78.390 kb on + strand at 78.391 kb on - strand at 78.409 kb on + strand at 78.410 kb on - strand at 78.410 kb on - strand at 78.410 kb on - strand at 78.410 kb on - strand at 78.482 kb on + strand at 78.483 kb on - strand at 78.483 kb on - strand at 78.484 kb on + strand at 78.484 kb on + strand at 78.484 kb on + strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.485 kb on - strand at 78.495 kb on + strand at 78.611 kb on + strand, within Pf6N2E2_39 at 78.708 kb on + strand, within Pf6N2E2_39 at 78.708 kb on + strand, within Pf6N2E2_39 at 78.709 kb on - strand, within Pf6N2E2_39 at 78.709 kb on - strand, within Pf6N2E2_39 at 78.714 kb on + strand, within Pf6N2E2_39 at 78.714 kb on + strand, within Pf6N2E2_39 at 78.715 kb on - strand, within Pf6N2E2_39 at 78.715 kb on - strand, within Pf6N2E2_39 at 78.735 kb on - strand, within Pf6N2E2_39 at 78.804 kb on - strand at 78.807 kb on + strand at 78.808 kb on - strand at 78.808 kb on - strand at 78.833 kb on - strand at 79.023 kb on - strand, within Pf6N2E2_40 at 79.023 kb on - strand, within Pf6N2E2_40 at 79.023 kb on - strand, within Pf6N2E2_40 at 79.025 kb on - strand, within Pf6N2E2_40 at 79.025 kb on - strand, within Pf6N2E2_40 at 79.025 kb on - strand, within Pf6N2E2_40 at 79.029 kb on - strand, within Pf6N2E2_40 at 79.099 kb on - strand at 79.108 kb on + strand at 79.109 kb on - strand at 79.230 kb on - strand, within Pf6N2E2_41 at 79.232 kb on - strand, within Pf6N2E2_41 at 79.252 kb on + strand, within Pf6N2E2_41 at 79.253 kb on - strand, within Pf6N2E2_41 at 79.411 kb on + strand, within Pf6N2E2_41 at 79.600 kb on + strand, within Pf6N2E2_41 at 79.600 kb on + strand, within Pf6N2E2_41 at 79.601 kb on - strand, within Pf6N2E2_41 at 79.601 kb on - strand, within Pf6N2E2_41 at 79.632 kb on + strand, within Pf6N2E2_41 at 79.633 kb on - strand, within Pf6N2E2_41 at 79.658 kb on - strand, within Pf6N2E2_41 at 79.658 kb on - strand, within Pf6N2E2_41 at 79.999 kb on - strand, within Pf6N2E2_42 at 80.023 kb on - strand, within Pf6N2E2_42 at 80.023 kb on - strand, within Pf6N2E2_42 at 80.023 kb on - strand, within Pf6N2E2_42
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 78,065 + Pf6N2E2_38 0.73 +0.0 78,065 + Pf6N2E2_38 0.73 -0.6 78,065 + Pf6N2E2_38 0.73 -0.2 78,065 + Pf6N2E2_38 0.73 +0.5 78,065 + Pf6N2E2_38 0.73 -0.1 78,065 + Pf6N2E2_38 0.73 +1.2 78,065 + Pf6N2E2_38 0.73 -0.9 78,066 - Pf6N2E2_38 0.73 -1.1 78,066 - Pf6N2E2_38 0.73 +0.5 78,066 - Pf6N2E2_38 0.73 +0.4 78,388 + +0.1 78,389 - -0.5 78,389 - +0.4 78,389 - +1.5 78,390 + -1.3 78,391 - +0.5 78,409 + -0.8 78,410 - +1.5 78,410 - +0.5 78,410 - +0.1 78,410 - +1.5 78,482 + +0.4 78,483 - +0.4 78,483 - -0.6 78,484 + -0.9 78,484 + -0.3 78,484 + -0.4 78,485 - -1.5 78,485 - +0.7 78,485 - +0.4 78,485 - +1.0 78,485 - +0.3 78,485 - -0.7 78,485 - +0.3 78,495 + -1.1 78,611 + Pf6N2E2_39 0.36 -0.9 78,708 + Pf6N2E2_39 0.67 +1.5 78,708 + Pf6N2E2_39 0.67 +0.8 78,709 - Pf6N2E2_39 0.67 +1.3 78,709 - Pf6N2E2_39 0.67 +0.4 78,714 + Pf6N2E2_39 0.69 +1.5 78,714 + Pf6N2E2_39 0.69 -1.2 78,715 - Pf6N2E2_39 0.69 +0.4 78,715 - Pf6N2E2_39 0.69 +0.4 78,735 - Pf6N2E2_39 0.76 +0.5 78,804 - -0.5 78,807 + -2.3 78,808 - +0.9 78,808 - -0.9 78,833 - -1.5 79,023 - Pf6N2E2_40 0.69 -2.9 79,023 - Pf6N2E2_40 0.69 -4.1 79,023 - Pf6N2E2_40 0.69 -3.5 79,025 - Pf6N2E2_40 0.69 -0.6 79,025 - Pf6N2E2_40 0.69 -0.9 79,025 - Pf6N2E2_40 0.69 -1.5 79,029 - Pf6N2E2_40 0.71 -1.0 79,099 - +1.5 79,108 + -1.2 79,109 - -0.3 79,230 - Pf6N2E2_41 0.16 -1.1 79,232 - Pf6N2E2_41 0.17 -2.2 79,252 + Pf6N2E2_41 0.20 -0.4 79,253 - Pf6N2E2_41 0.20 +0.8 79,411 + Pf6N2E2_41 0.43 +0.3 79,600 + Pf6N2E2_41 0.72 +0.6 79,600 + Pf6N2E2_41 0.72 -0.6 79,601 - Pf6N2E2_41 0.72 -0.3 79,601 - Pf6N2E2_41 0.72 -1.0 79,632 + Pf6N2E2_41 0.77 +1.8 79,633 - Pf6N2E2_41 0.77 -0.8 79,658 - Pf6N2E2_41 0.80 -0.8 79,658 - Pf6N2E2_41 0.80 -0.9 79,999 - Pf6N2E2_42 0.16 -0.6 80,023 - Pf6N2E2_42 0.18 -0.2 80,023 - Pf6N2E2_42 0.18 -1.6 80,023 - Pf6N2E2_42 0.18 +1.5
Or see this region's nucleotide sequence