Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2735 and Pf6N2E2_2736 overlap by 4 nucleotides Pf6N2E2_2736 and Pf6N2E2_2737 overlap by 4 nucleotides Pf6N2E2_2737 and Pf6N2E2_2738 are separated by 2 nucleotides
Pf6N2E2_2735 - hypothetical protein, at 3,226,543 to 3,228,630
_2735
Pf6N2E2_2736 - hypothetical protein, at 3,228,627 to 3,229,010
_2736
Pf6N2E2_2737 - SAM-dependent methyltransferases, at 3,229,007 to 3,229,717
_2737
Pf6N2E2_2738 - Glycosyltransferase, at 3,229,720 to 3,230,688
_2738
Position (kb)
3229
3230 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3228.050 kb on + strand, within Pf6N2E2_2735 at 3228.051 kb on - strand, within Pf6N2E2_2735 at 3228.079 kb on + strand, within Pf6N2E2_2735 at 3228.131 kb on + strand, within Pf6N2E2_2735 at 3228.132 kb on - strand, within Pf6N2E2_2735 at 3228.132 kb on - strand, within Pf6N2E2_2735 at 3228.132 kb on - strand, within Pf6N2E2_2735 at 3228.425 kb on + strand at 3228.426 kb on - strand at 3228.488 kb on + strand at 3228.488 kb on + strand at 3228.488 kb on + strand at 3228.488 kb on + strand at 3228.488 kb on + strand at 3228.489 kb on - strand at 3228.489 kb on - strand at 3228.489 kb on - strand at 3228.707 kb on - strand, within Pf6N2E2_2736 at 3228.825 kb on + strand, within Pf6N2E2_2736 at 3228.825 kb on + strand, within Pf6N2E2_2736 at 3228.826 kb on - strand, within Pf6N2E2_2736 at 3228.939 kb on + strand, within Pf6N2E2_2736 at 3228.940 kb on - strand, within Pf6N2E2_2736 at 3228.940 kb on - strand, within Pf6N2E2_2736 at 3229.049 kb on - strand at 3229.196 kb on + strand, within Pf6N2E2_2737 at 3229.197 kb on - strand, within Pf6N2E2_2737 at 3229.197 kb on - strand, within Pf6N2E2_2737 at 3229.210 kb on - strand, within Pf6N2E2_2737 at 3229.210 kb on - strand, within Pf6N2E2_2737 at 3229.212 kb on + strand, within Pf6N2E2_2737 at 3229.212 kb on + strand, within Pf6N2E2_2737 at 3229.213 kb on - strand, within Pf6N2E2_2737 at 3229.275 kb on + strand, within Pf6N2E2_2737 at 3229.276 kb on - strand, within Pf6N2E2_2737 at 3229.276 kb on - strand, within Pf6N2E2_2737 at 3229.276 kb on - strand, within Pf6N2E2_2737 at 3229.377 kb on + strand, within Pf6N2E2_2737 at 3229.377 kb on + strand, within Pf6N2E2_2737 at 3229.377 kb on + strand, within Pf6N2E2_2737 at 3229.377 kb on + strand, within Pf6N2E2_2737 at 3229.378 kb on - strand, within Pf6N2E2_2737 at 3229.609 kb on + strand, within Pf6N2E2_2737 at 3229.665 kb on + strand at 3229.666 kb on - strand at 3229.736 kb on + strand at 3229.736 kb on + strand at 3229.736 kb on + strand at 3229.736 kb on + strand at 3229.736 kb on + strand at 3229.737 kb on - strand at 3229.737 kb on - strand at 3229.781 kb on + strand at 3229.782 kb on - strand at 3229.782 kb on - strand at 3229.782 kb on - strand at 3229.782 kb on - strand at 3229.796 kb on + strand at 3229.796 kb on + strand at 3229.869 kb on - strand, within Pf6N2E2_2738 at 3229.882 kb on + strand, within Pf6N2E2_2738 at 3229.883 kb on - strand, within Pf6N2E2_2738 at 3229.906 kb on + strand, within Pf6N2E2_2738 at 3229.907 kb on - strand, within Pf6N2E2_2738 at 3229.958 kb on + strand, within Pf6N2E2_2738 at 3229.958 kb on + strand, within Pf6N2E2_2738 at 3229.958 kb on + strand, within Pf6N2E2_2738 at 3229.959 kb on - strand, within Pf6N2E2_2738 at 3229.959 kb on - strand, within Pf6N2E2_2738 at 3229.959 kb on - strand, within Pf6N2E2_2738 at 3229.968 kb on + strand, within Pf6N2E2_2738 at 3229.968 kb on + strand, within Pf6N2E2_2738 at 3229.969 kb on - strand, within Pf6N2E2_2738 at 3230.072 kb on + strand, within Pf6N2E2_2738 at 3230.072 kb on + strand, within Pf6N2E2_2738 at 3230.073 kb on - strand, within Pf6N2E2_2738 at 3230.073 kb on - strand, within Pf6N2E2_2738 at 3230.073 kb on - strand, within Pf6N2E2_2738 at 3230.073 kb on - strand, within Pf6N2E2_2738 at 3230.081 kb on + strand, within Pf6N2E2_2738 at 3230.081 kb on + strand, within Pf6N2E2_2738 at 3230.081 kb on + strand, within Pf6N2E2_2738 at 3230.081 kb on + strand, within Pf6N2E2_2738 at 3230.082 kb on - strand, within Pf6N2E2_2738 at 3230.082 kb on - strand, within Pf6N2E2_2738 at 3230.082 kb on - strand, within Pf6N2E2_2738 at 3230.099 kb on - strand, within Pf6N2E2_2738 at 3230.224 kb on - strand, within Pf6N2E2_2738 at 3230.237 kb on + strand, within Pf6N2E2_2738 at 3230.625 kb on - strand at 3230.625 kb on - strand at 3230.625 kb on - strand at 3230.651 kb on - strand at 3230.693 kb on + strand at 3230.693 kb on + strand at 3230.694 kb on - strand at 3230.694 kb on - strand at 3230.694 kb on - strand at 3230.706 kb on + strand at 3230.706 kb on + strand at 3230.706 kb on + strand at 3230.708 kb on + strand at 3230.708 kb on + strand at 3230.708 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 3,228,050 + Pf6N2E2_2735 0.72 +0.2 3,228,051 - Pf6N2E2_2735 0.72 +0.3 3,228,079 + Pf6N2E2_2735 0.74 +0.3 3,228,131 + Pf6N2E2_2735 0.76 +1.1 3,228,132 - Pf6N2E2_2735 0.76 +0.3 3,228,132 - Pf6N2E2_2735 0.76 -0.9 3,228,132 - Pf6N2E2_2735 0.76 +0.7 3,228,425 + +0.7 3,228,426 - -0.8 3,228,488 + -1.4 3,228,488 + -0.4 3,228,488 + -0.3 3,228,488 + +0.3 3,228,488 + +0.5 3,228,489 - +0.3 3,228,489 - -0.5 3,228,489 - +1.5 3,228,707 - Pf6N2E2_2736 0.21 -3.0 3,228,825 + Pf6N2E2_2736 0.52 +0.7 3,228,825 + Pf6N2E2_2736 0.52 -0.6 3,228,826 - Pf6N2E2_2736 0.52 -0.1 3,228,939 + Pf6N2E2_2736 0.81 -0.5 3,228,940 - Pf6N2E2_2736 0.82 -0.5 3,228,940 - Pf6N2E2_2736 0.82 -1.0 3,229,049 - -1.4 3,229,196 + Pf6N2E2_2737 0.27 -0.5 3,229,197 - Pf6N2E2_2737 0.27 -0.1 3,229,197 - Pf6N2E2_2737 0.27 +2.5 3,229,210 - Pf6N2E2_2737 0.29 -0.7 3,229,210 - Pf6N2E2_2737 0.29 -0.7 3,229,212 + Pf6N2E2_2737 0.29 -0.4 3,229,212 + Pf6N2E2_2737 0.29 +0.5 3,229,213 - Pf6N2E2_2737 0.29 -0.8 3,229,275 + Pf6N2E2_2737 0.38 -0.1 3,229,276 - Pf6N2E2_2737 0.38 -0.8 3,229,276 - Pf6N2E2_2737 0.38 -0.1 3,229,276 - Pf6N2E2_2737 0.38 -1.0 3,229,377 + Pf6N2E2_2737 0.52 -0.8 3,229,377 + Pf6N2E2_2737 0.52 -0.5 3,229,377 + Pf6N2E2_2737 0.52 -0.2 3,229,377 + Pf6N2E2_2737 0.52 -0.3 3,229,378 - Pf6N2E2_2737 0.52 +0.5 3,229,609 + Pf6N2E2_2737 0.85 -0.1 3,229,665 + -0.4 3,229,666 - +0.7 3,229,736 + -0.5 3,229,736 + -0.8 3,229,736 + +1.0 3,229,736 + -0.6 3,229,736 + +0.1 3,229,737 - +0.5 3,229,737 - -1.0 3,229,781 + +1.5 3,229,782 - -1.6 3,229,782 - -0.1 3,229,782 - -1.3 3,229,782 - +2.1 3,229,796 + +1.5 3,229,796 + -0.1 3,229,869 - Pf6N2E2_2738 0.15 +0.5 3,229,882 + Pf6N2E2_2738 0.17 -0.3 3,229,883 - Pf6N2E2_2738 0.17 -0.2 3,229,906 + Pf6N2E2_2738 0.19 +0.7 3,229,907 - Pf6N2E2_2738 0.19 +0.1 3,229,958 + Pf6N2E2_2738 0.25 +1.5 3,229,958 + Pf6N2E2_2738 0.25 +1.5 3,229,958 + Pf6N2E2_2738 0.25 +0.3 3,229,959 - Pf6N2E2_2738 0.25 -0.7 3,229,959 - Pf6N2E2_2738 0.25 -0.2 3,229,959 - Pf6N2E2_2738 0.25 -0.3 3,229,968 + Pf6N2E2_2738 0.26 +0.1 3,229,968 + Pf6N2E2_2738 0.26 +0.2 3,229,969 - Pf6N2E2_2738 0.26 +0.0 3,230,072 + Pf6N2E2_2738 0.36 +0.2 3,230,072 + Pf6N2E2_2738 0.36 -0.2 3,230,073 - Pf6N2E2_2738 0.36 -0.5 3,230,073 - Pf6N2E2_2738 0.36 -0.2 3,230,073 - Pf6N2E2_2738 0.36 +0.6 3,230,073 - Pf6N2E2_2738 0.36 +0.4 3,230,081 + Pf6N2E2_2738 0.37 -0.2 3,230,081 + Pf6N2E2_2738 0.37 -0.4 3,230,081 + Pf6N2E2_2738 0.37 -0.0 3,230,081 + Pf6N2E2_2738 0.37 -0.3 3,230,082 - Pf6N2E2_2738 0.37 -0.2 3,230,082 - Pf6N2E2_2738 0.37 -0.6 3,230,082 - Pf6N2E2_2738 0.37 +0.5 3,230,099 - Pf6N2E2_2738 0.39 -0.9 3,230,224 - Pf6N2E2_2738 0.52 +1.4 3,230,237 + Pf6N2E2_2738 0.53 +0.8 3,230,625 - +2.5 3,230,625 - +0.5 3,230,625 - +0.6 3,230,651 - +2.5 3,230,693 + +1.3 3,230,693 + -0.1 3,230,694 - +0.3 3,230,694 - -2.7 3,230,694 - -0.5 3,230,706 + -0.1 3,230,706 + -0.1 3,230,706 + +0.1 3,230,708 + -0.2 3,230,708 + +0.3 3,230,708 + -1.9
Or see this region's nucleotide sequence