Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_236 and Pf6N2E2_237 are separated by 291 nucleotides Pf6N2E2_237 and Pf6N2E2_238 are separated by 219 nucleotides Pf6N2E2_238 and Pf6N2E2_239 are separated by 33 nucleotides Pf6N2E2_239 and Pf6N2E2_240 are separated by 171 nucleotides
Pf6N2E2_236 - putative transcriptional regulator protein, at 298,600 to 299,253
_236
Pf6N2E2_237 - hypothetical protein, at 299,545 to 299,757
_237
Pf6N2E2_238 - hypothetical protein, at 299,977 to 300,096
_238
Pf6N2E2_239 - Fatty acid desaturase, at 300,130 to 300,831
_239
Pf6N2E2_240 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 301,003 to 301,893
_240
Position (kb) 299
300
301 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 299.089 kb on + strand, within Pf6N2E2_236 at 299.090 kb on - strand, within Pf6N2E2_236 at 299.116 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.130 kb on - strand, within Pf6N2E2_236 at 299.130 kb on - strand, within Pf6N2E2_236 at 299.165 kb on + strand, within Pf6N2E2_236 at 299.272 kb on + strand at 299.272 kb on + strand at 299.301 kb on - strand at 299.307 kb on + strand at 299.392 kb on + strand at 299.393 kb on - strand at 299.413 kb on + strand at 299.414 kb on - strand at 299.491 kb on + strand at 299.491 kb on + strand at 299.491 kb on + strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.538 kb on + strand at 299.539 kb on - strand at 299.539 kb on - strand at 299.539 kb on - strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.545 kb on - strand at 299.545 kb on - strand at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.747 kb on - strand at 299.786 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.813 kb on - strand at 299.813 kb on - strand at 299.871 kb on - strand at 299.880 kb on + strand at 299.880 kb on + strand at 299.880 kb on + strand at 299.881 kb on - strand at 299.881 kb on - strand at 299.881 kb on - strand at 299.896 kb on + strand at 299.978 kb on + strand at 299.979 kb on - strand at 299.992 kb on + strand, within Pf6N2E2_238 at 299.992 kb on + strand, within Pf6N2E2_238 at 300.010 kb on - strand, within Pf6N2E2_238 at 300.172 kb on + strand at 300.183 kb on + strand at 300.184 kb on - strand at 300.184 kb on - strand at 300.295 kb on + strand, within Pf6N2E2_239 at 300.295 kb on + strand, within Pf6N2E2_239 at 300.296 kb on - strand, within Pf6N2E2_239 at 300.296 kb on - strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.482 kb on - strand, within Pf6N2E2_239 at 300.482 kb on - strand, within Pf6N2E2_239 at 300.506 kb on - strand, within Pf6N2E2_239 at 300.506 kb on - strand, within Pf6N2E2_239 at 300.559 kb on + strand, within Pf6N2E2_239 at 300.595 kb on - strand, within Pf6N2E2_239 at 300.595 kb on - strand, within Pf6N2E2_239 at 300.707 kb on - strand, within Pf6N2E2_239 at 300.707 kb on - strand, within Pf6N2E2_239 at 300.709 kb on - strand, within Pf6N2E2_239 at 300.749 kb on - strand, within Pf6N2E2_239 at 300.764 kb on - strand at 300.764 kb on - strand at 300.773 kb on + strand at 300.830 kb on - strand at 300.835 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.845 kb on - strand at 300.845 kb on - strand at 300.894 kb on + strand at 300.894 kb on + strand at 300.960 kb on - strand at 300.960 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 299,089 + Pf6N2E2_236 0.75 +0.2 299,090 - Pf6N2E2_236 0.75 -0.3 299,116 + Pf6N2E2_236 0.79 -1.7 299,129 + Pf6N2E2_236 0.81 -0.6 299,129 + Pf6N2E2_236 0.81 -0.5 299,129 + Pf6N2E2_236 0.81 -0.4 299,130 - Pf6N2E2_236 0.81 -0.1 299,130 - Pf6N2E2_236 0.81 +0.6 299,165 + Pf6N2E2_236 0.86 -0.2 299,272 + -1.2 299,272 + -1.5 299,301 - +0.6 299,307 + +0.4 299,392 + -0.6 299,393 - -0.4 299,413 + -1.2 299,414 - -0.9 299,491 + +0.6 299,491 + +0.5 299,491 + +1.5 299,492 - +1.5 299,492 - -2.5 299,492 - +0.5 299,492 - +0.5 299,492 - -0.7 299,492 - +0.9 299,492 - +1.5 299,492 - -0.7 299,492 - -0.4 299,538 + +0.1 299,539 - +1.5 299,539 - +0.0 299,539 - +3.1 299,544 + -0.8 299,544 + -0.7 299,544 + +0.2 299,544 + +1.5 299,545 - +0.2 299,545 - +1.5 299,591 - Pf6N2E2_237 0.22 +0.0 299,591 - Pf6N2E2_237 0.22 -0.1 299,591 - Pf6N2E2_237 0.22 -0.9 299,591 - Pf6N2E2_237 0.22 -0.6 299,747 - +0.2 299,786 - +1.9 299,810 - -0.3 299,810 - +1.1 299,810 - +0.5 299,810 - +0.6 299,810 - -0.8 299,813 - -0.1 299,813 - -1.4 299,871 - +0.5 299,880 + +1.5 299,880 + -1.1 299,880 + -2.4 299,881 - -1.3 299,881 - +0.6 299,881 - +0.0 299,896 + +0.2 299,978 + -1.2 299,979 - -0.9 299,992 + Pf6N2E2_238 0.12 +0.5 299,992 + Pf6N2E2_238 0.12 -0.1 300,010 - Pf6N2E2_238 0.28 -0.1 300,172 + +1.5 300,183 + +1.5 300,184 - +0.5 300,184 - +0.2 300,295 + Pf6N2E2_239 0.24 -0.9 300,295 + Pf6N2E2_239 0.24 +1.5 300,296 - Pf6N2E2_239 0.24 +0.0 300,296 - Pf6N2E2_239 0.24 -0.2 300,297 + Pf6N2E2_239 0.24 -0.9 300,297 + Pf6N2E2_239 0.24 -0.8 300,297 + Pf6N2E2_239 0.24 -1.5 300,298 - Pf6N2E2_239 0.24 +0.1 300,298 - Pf6N2E2_239 0.24 +0.5 300,298 - Pf6N2E2_239 0.24 +1.5 300,298 - Pf6N2E2_239 0.24 -0.9 300,298 - Pf6N2E2_239 0.24 +0.2 300,298 - Pf6N2E2_239 0.24 -0.1 300,298 - Pf6N2E2_239 0.24 +0.1 300,482 - Pf6N2E2_239 0.50 -0.1 300,482 - Pf6N2E2_239 0.50 +1.2 300,506 - Pf6N2E2_239 0.54 +1.5 300,506 - Pf6N2E2_239 0.54 +0.1 300,559 + Pf6N2E2_239 0.61 -0.4 300,595 - Pf6N2E2_239 0.66 -0.7 300,595 - Pf6N2E2_239 0.66 +1.5 300,707 - Pf6N2E2_239 0.82 -1.9 300,707 - Pf6N2E2_239 0.82 -0.5 300,709 - Pf6N2E2_239 0.82 +1.5 300,749 - Pf6N2E2_239 0.88 +1.5 300,764 - +0.5 300,764 - -1.4 300,773 + +0.5 300,830 - -0.4 300,835 + -0.4 300,844 + +1.5 300,844 + +0.9 300,844 + -0.4 300,844 + -0.3 300,845 - +1.1 300,845 - -0.1 300,894 + -0.7 300,894 + +0.7 300,960 - -0.1 300,960 - +0.5
Or see this region's nucleotide sequence