Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2217 and Pf6N2E2_2218 are separated by 107 nucleotides Pf6N2E2_2218 and Pf6N2E2_2219 are separated by 93 nucleotides
Pf6N2E2_2217 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 2,628,296 to 2,629,579
_2217
Pf6N2E2_2218 - ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92), at 2,629,687 to 2,630,328
_2218
Pf6N2E2_2219 - Cell division trigger factor (EC 5.2.1.8), at 2,630,422 to 2,631,732
_2219
Position (kb)
2629
2630
2631 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2629.030 kb on - strand, within Pf6N2E2_2217 at 2629.030 kb on - strand, within Pf6N2E2_2217 at 2629.063 kb on - strand, within Pf6N2E2_2217 at 2629.063 kb on - strand, within Pf6N2E2_2217 at 2629.300 kb on - strand, within Pf6N2E2_2217 at 2629.300 kb on - strand, within Pf6N2E2_2217 at 2629.300 kb on - strand, within Pf6N2E2_2217 at 2629.348 kb on - strand, within Pf6N2E2_2217 at 2629.390 kb on + strand, within Pf6N2E2_2217 at 2629.536 kb on + strand at 2629.537 kb on - strand at 2629.676 kb on + strand at 2630.048 kb on + strand, within Pf6N2E2_2218 at 2630.093 kb on + strand, within Pf6N2E2_2218 at 2630.093 kb on + strand, within Pf6N2E2_2218 at 2630.093 kb on + strand, within Pf6N2E2_2218 at 2630.093 kb on + strand, within Pf6N2E2_2218 at 2630.093 kb on + strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.094 kb on - strand, within Pf6N2E2_2218 at 2630.165 kb on + strand, within Pf6N2E2_2218 at 2630.219 kb on + strand, within Pf6N2E2_2218 at 2630.219 kb on + strand, within Pf6N2E2_2218 at 2630.220 kb on - strand, within Pf6N2E2_2218 at 2630.220 kb on - strand, within Pf6N2E2_2218 at 2630.349 kb on + strand at 2630.349 kb on + strand at 2630.350 kb on - strand at 2630.354 kb on - strand at 2630.354 kb on - strand at 2630.354 kb on - strand at 2630.354 kb on - strand at 2630.354 kb on - strand at 2630.365 kb on - strand at 2630.371 kb on - strand at 2630.393 kb on - strand at 2630.403 kb on - strand at 2630.466 kb on - strand at 2630.466 kb on - strand at 2630.509 kb on - strand at 2630.571 kb on - strand, within Pf6N2E2_2219 at 2631.072 kb on - strand, within Pf6N2E2_2219
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 2,629,030 - Pf6N2E2_2217 0.57 -0.5 2,629,030 - Pf6N2E2_2217 0.57 -1.1 2,629,063 - Pf6N2E2_2217 0.60 -1.5 2,629,063 - Pf6N2E2_2217 0.60 -2.2 2,629,300 - Pf6N2E2_2217 0.78 -2.6 2,629,300 - Pf6N2E2_2217 0.78 -1.5 2,629,300 - Pf6N2E2_2217 0.78 -1.1 2,629,348 - Pf6N2E2_2217 0.82 -1.2 2,629,390 + Pf6N2E2_2217 0.85 -1.5 2,629,536 + -2.2 2,629,537 - -1.8 2,629,676 + -0.5 2,630,048 + Pf6N2E2_2218 0.56 +1.5 2,630,093 + Pf6N2E2_2218 0.63 -1.6 2,630,093 + Pf6N2E2_2218 0.63 +0.5 2,630,093 + Pf6N2E2_2218 0.63 +1.5 2,630,093 + Pf6N2E2_2218 0.63 -1.1 2,630,093 + Pf6N2E2_2218 0.63 -1.9 2,630,094 - Pf6N2E2_2218 0.63 -1.5 2,630,094 - Pf6N2E2_2218 0.63 -0.8 2,630,094 - Pf6N2E2_2218 0.63 -1.3 2,630,094 - Pf6N2E2_2218 0.63 -2.3 2,630,094 - Pf6N2E2_2218 0.63 +0.2 2,630,094 - Pf6N2E2_2218 0.63 +0.5 2,630,165 + Pf6N2E2_2218 0.74 -2.7 2,630,219 + Pf6N2E2_2218 0.83 -1.9 2,630,219 + Pf6N2E2_2218 0.83 -0.1 2,630,220 - Pf6N2E2_2218 0.83 -2.4 2,630,220 - Pf6N2E2_2218 0.83 -1.6 2,630,349 + +1.5 2,630,349 + -1.5 2,630,350 - -1.0 2,630,354 - +0.3 2,630,354 - +0.1 2,630,354 - -0.1 2,630,354 - +0.4 2,630,354 - -3.0 2,630,365 - -0.1 2,630,371 - +0.7 2,630,393 - -0.3 2,630,403 - +0.5 2,630,466 - +1.5 2,630,466 - +2.5 2,630,509 - +1.5 2,630,571 - Pf6N2E2_2219 0.11 +1.5 2,631,072 - Pf6N2E2_2219 0.50 +1.5
Or see this region's nucleotide sequence