Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2187 and Pf6N2E2_2188 are separated by 197 nucleotides Pf6N2E2_2188 and Pf6N2E2_2189 are separated by 92 nucleotides
Pf6N2E2_2187 - Transcriptional regulator, LysR family, at 2,591,642 to 2,592,571
_2187
Pf6N2E2_2188 - Predicted transcriptional regulator LiuR of leucine degradation pathway, MerR family, at 2,592,769 to 2,593,170
_2188
Pf6N2E2_2189 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 2,593,263 to 2,594,162
_2189
Position (kb)
2592
2593
2594 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2592.013 kb on + strand, within Pf6N2E2_2187 at 2592.013 kb on + strand, within Pf6N2E2_2187 at 2592.014 kb on - strand, within Pf6N2E2_2187 at 2592.014 kb on - strand, within Pf6N2E2_2187 at 2592.014 kb on - strand, within Pf6N2E2_2187 at 2592.123 kb on + strand, within Pf6N2E2_2187 at 2592.152 kb on + strand, within Pf6N2E2_2187 at 2592.152 kb on + strand, within Pf6N2E2_2187 at 2592.153 kb on - strand, within Pf6N2E2_2187 at 2592.153 kb on - strand, within Pf6N2E2_2187 at 2592.215 kb on + strand, within Pf6N2E2_2187 at 2592.238 kb on + strand, within Pf6N2E2_2187 at 2592.238 kb on + strand, within Pf6N2E2_2187 at 2592.239 kb on - strand, within Pf6N2E2_2187 at 2592.239 kb on - strand, within Pf6N2E2_2187 at 2592.266 kb on + strand, within Pf6N2E2_2187 at 2592.267 kb on - strand, within Pf6N2E2_2187 at 2592.267 kb on - strand, within Pf6N2E2_2187 at 2592.267 kb on - strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.330 kb on - strand, within Pf6N2E2_2187 at 2592.330 kb on - strand, within Pf6N2E2_2187 at 2592.570 kb on - strand at 2592.631 kb on - strand at 2592.631 kb on - strand at 2592.643 kb on - strand at 2592.721 kb on - strand at 2592.732 kb on + strand at 2592.737 kb on + strand at 2592.784 kb on + strand at 2592.784 kb on + strand at 2592.785 kb on - strand at 2592.785 kb on - strand at 2592.785 kb on - strand at 2592.785 kb on - strand at 2592.841 kb on + strand, within Pf6N2E2_2188 at 2592.842 kb on - strand, within Pf6N2E2_2188 at 2592.842 kb on - strand, within Pf6N2E2_2188 at 2592.892 kb on + strand, within Pf6N2E2_2188 at 2592.986 kb on - strand, within Pf6N2E2_2188 at 2592.986 kb on - strand, within Pf6N2E2_2188 at 2592.986 kb on - strand, within Pf6N2E2_2188 at 2593.169 kb on - strand at 2593.234 kb on + strand at 2593.234 kb on + strand at 2593.234 kb on + strand at 2593.235 kb on - strand at 2593.235 kb on - strand at 2593.283 kb on - strand at 2593.283 kb on - strand at 2593.392 kb on + strand, within Pf6N2E2_2189 at 2593.486 kb on + strand, within Pf6N2E2_2189 at 2593.486 kb on + strand, within Pf6N2E2_2189 at 2593.491 kb on + strand, within Pf6N2E2_2189 at 2593.491 kb on + strand, within Pf6N2E2_2189 at 2593.492 kb on - strand, within Pf6N2E2_2189 at 2593.762 kb on - strand, within Pf6N2E2_2189 at 2593.762 kb on - strand, within Pf6N2E2_2189 at 2593.776 kb on + strand, within Pf6N2E2_2189 at 2593.896 kb on + strand, within Pf6N2E2_2189 at 2593.896 kb on + strand, within Pf6N2E2_2189 at 2593.896 kb on + strand, within Pf6N2E2_2189 at 2593.897 kb on - strand, within Pf6N2E2_2189 at 2593.986 kb on + strand, within Pf6N2E2_2189 at 2594.031 kb on + strand, within Pf6N2E2_2189 at 2594.031 kb on + strand, within Pf6N2E2_2189 at 2594.032 kb on - strand, within Pf6N2E2_2189 at 2594.032 kb on - strand, within Pf6N2E2_2189 at 2594.032 kb on - strand, within Pf6N2E2_2189 at 2594.032 kb on - strand, within Pf6N2E2_2189 at 2594.032 kb on - strand, within Pf6N2E2_2189
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 2,592,013 + Pf6N2E2_2187 0.40 -0.2 2,592,013 + Pf6N2E2_2187 0.40 +0.5 2,592,014 - Pf6N2E2_2187 0.40 -2.2 2,592,014 - Pf6N2E2_2187 0.40 +0.4 2,592,014 - Pf6N2E2_2187 0.40 -0.3 2,592,123 + Pf6N2E2_2187 0.52 +0.3 2,592,152 + Pf6N2E2_2187 0.55 -1.5 2,592,152 + Pf6N2E2_2187 0.55 +0.2 2,592,153 - Pf6N2E2_2187 0.55 -0.1 2,592,153 - Pf6N2E2_2187 0.55 +0.5 2,592,215 + Pf6N2E2_2187 0.62 +0.3 2,592,238 + Pf6N2E2_2187 0.64 +0.8 2,592,238 + Pf6N2E2_2187 0.64 -0.0 2,592,239 - Pf6N2E2_2187 0.64 -0.0 2,592,239 - Pf6N2E2_2187 0.64 -0.1 2,592,266 + Pf6N2E2_2187 0.67 +1.5 2,592,267 - Pf6N2E2_2187 0.67 +0.0 2,592,267 - Pf6N2E2_2187 0.67 -0.3 2,592,267 - Pf6N2E2_2187 0.67 +0.0 2,592,329 + Pf6N2E2_2187 0.74 -0.3 2,592,329 + Pf6N2E2_2187 0.74 +0.6 2,592,329 + Pf6N2E2_2187 0.74 -0.6 2,592,329 + Pf6N2E2_2187 0.74 -0.0 2,592,329 + Pf6N2E2_2187 0.74 -0.3 2,592,330 - Pf6N2E2_2187 0.74 +0.3 2,592,330 - Pf6N2E2_2187 0.74 +0.5 2,592,570 - -1.3 2,592,631 - -0.5 2,592,631 - +0.5 2,592,643 - +0.5 2,592,721 - +0.5 2,592,732 + -0.4 2,592,737 + +0.1 2,592,784 + +1.5 2,592,784 + -0.5 2,592,785 - -1.6 2,592,785 - -0.3 2,592,785 - -2.9 2,592,785 - -0.0 2,592,841 + Pf6N2E2_2188 0.18 -0.7 2,592,842 - Pf6N2E2_2188 0.18 -0.3 2,592,842 - Pf6N2E2_2188 0.18 -0.3 2,592,892 + Pf6N2E2_2188 0.31 -1.0 2,592,986 - Pf6N2E2_2188 0.54 -1.6 2,592,986 - Pf6N2E2_2188 0.54 -2.4 2,592,986 - Pf6N2E2_2188 0.54 -2.4 2,593,169 - +1.5 2,593,234 + -0.0 2,593,234 + -0.3 2,593,234 + -0.4 2,593,235 - +1.5 2,593,235 - -0.0 2,593,283 - -1.0 2,593,283 - +0.5 2,593,392 + Pf6N2E2_2189 0.14 -1.9 2,593,486 + Pf6N2E2_2189 0.25 +1.5 2,593,486 + Pf6N2E2_2189 0.25 +0.5 2,593,491 + Pf6N2E2_2189 0.25 -0.8 2,593,491 + Pf6N2E2_2189 0.25 -2.0 2,593,492 - Pf6N2E2_2189 0.25 -2.2 2,593,762 - Pf6N2E2_2189 0.55 -1.5 2,593,762 - Pf6N2E2_2189 0.55 +1.5 2,593,776 + Pf6N2E2_2189 0.57 +1.5 2,593,896 + Pf6N2E2_2189 0.70 -1.0 2,593,896 + Pf6N2E2_2189 0.70 -2.0 2,593,896 + Pf6N2E2_2189 0.70 -1.5 2,593,897 - Pf6N2E2_2189 0.70 -1.6 2,593,986 + Pf6N2E2_2189 0.80 +1.1 2,594,031 + Pf6N2E2_2189 0.85 -1.0 2,594,031 + Pf6N2E2_2189 0.85 -1.0 2,594,032 - Pf6N2E2_2189 0.85 -0.8 2,594,032 - Pf6N2E2_2189 0.85 -0.0 2,594,032 - Pf6N2E2_2189 0.85 -0.3 2,594,032 - Pf6N2E2_2189 0.85 -2.0 2,594,032 - Pf6N2E2_2189 0.85 +1.5
Or see this region's nucleotide sequence