Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2179 and Pf6N2E2_2180 are separated by 110 nucleotides Pf6N2E2_2180 and Pf6N2E2_2181 are separated by 61 nucleotides
Pf6N2E2_2179 - Putrescine importer, at 2,583,681 to 2,585,030
_2179
Pf6N2E2_2180 - Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109), at 2,585,141 to 2,586,157
_2180
Pf6N2E2_2181 - Arginine N-succinyltransferase (EC 2.3.1.109), at 2,586,219 to 2,587,253
_2181
Position (kb)
2585
2586
2587 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2584.192 kb on - strand, within Pf6N2E2_2179 at 2584.192 kb on - strand, within Pf6N2E2_2179 at 2584.251 kb on + strand, within Pf6N2E2_2179 at 2584.683 kb on + strand, within Pf6N2E2_2179 at 2584.683 kb on + strand, within Pf6N2E2_2179 at 2584.684 kb on - strand, within Pf6N2E2_2179 at 2584.696 kb on - strand, within Pf6N2E2_2179 at 2584.696 kb on - strand, within Pf6N2E2_2179 at 2584.728 kb on + strand, within Pf6N2E2_2179 at 2584.729 kb on - strand, within Pf6N2E2_2179 at 2584.824 kb on + strand, within Pf6N2E2_2179 at 2584.824 kb on + strand, within Pf6N2E2_2179 at 2584.908 kb on + strand at 2584.908 kb on + strand at 2584.908 kb on + strand at 2584.908 kb on + strand at 2584.908 kb on + strand at 2584.908 kb on + strand at 2584.908 kb on + strand at 2584.908 kb on + strand at 2584.909 kb on - strand at 2584.968 kb on + strand at 2584.968 kb on + strand at 2584.968 kb on + strand at 2585.065 kb on + strand at 2585.065 kb on + strand at 2585.065 kb on + strand at 2585.065 kb on + strand at 2585.065 kb on + strand at 2585.066 kb on - strand at 2585.151 kb on + strand at 2585.167 kb on - strand at 2585.167 kb on - strand at 2585.388 kb on - strand, within Pf6N2E2_2180 at 2585.402 kb on + strand, within Pf6N2E2_2180 at 2585.781 kb on - strand, within Pf6N2E2_2180 at 2585.781 kb on - strand, within Pf6N2E2_2180 at 2585.781 kb on - strand, within Pf6N2E2_2180 at 2585.781 kb on - strand, within Pf6N2E2_2180 at 2585.888 kb on + strand, within Pf6N2E2_2180 at 2585.888 kb on + strand at 2585.889 kb on - strand, within Pf6N2E2_2180 at 2585.889 kb on - strand, within Pf6N2E2_2180 at 2585.896 kb on + strand at 2586.038 kb on + strand, within Pf6N2E2_2180 at 2586.153 kb on + strand at 2586.201 kb on + strand at 2586.201 kb on + strand at 2586.201 kb on + strand at 2586.201 kb on + strand at 2586.202 kb on - strand at 2586.202 kb on - strand at 2586.202 kb on - strand at 2586.202 kb on - strand at 2586.202 kb on - strand at 2586.202 kb on - strand at 2586.472 kb on - strand at 2586.472 kb on - strand, within Pf6N2E2_2181 at 2586.477 kb on + strand, within Pf6N2E2_2181 at 2586.850 kb on - strand, within Pf6N2E2_2181
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 2,584,192 - Pf6N2E2_2179 0.38 -0.4 2,584,192 - Pf6N2E2_2179 0.38 -1.6 2,584,251 + Pf6N2E2_2179 0.42 -1.0 2,584,683 + Pf6N2E2_2179 0.74 +0.5 2,584,683 + Pf6N2E2_2179 0.74 -0.1 2,584,684 - Pf6N2E2_2179 0.74 -2.0 2,584,696 - Pf6N2E2_2179 0.75 -0.6 2,584,696 - Pf6N2E2_2179 0.75 -3.2 2,584,728 + Pf6N2E2_2179 0.78 +0.8 2,584,729 - Pf6N2E2_2179 0.78 +0.1 2,584,824 + Pf6N2E2_2179 0.85 +0.2 2,584,824 + Pf6N2E2_2179 0.85 +2.1 2,584,908 + -0.2 2,584,908 + +1.0 2,584,908 + -0.4 2,584,908 + +1.5 2,584,908 + +0.5 2,584,908 + +1.3 2,584,908 + +0.1 2,584,908 + +0.4 2,584,909 - -0.5 2,584,968 + +0.8 2,584,968 + +0.5 2,584,968 + +0.2 2,585,065 + +0.7 2,585,065 + +0.1 2,585,065 + +0.1 2,585,065 + -0.0 2,585,065 + -0.7 2,585,066 - -2.7 2,585,151 + -2.1 2,585,167 - +0.0 2,585,167 - +0.9 2,585,388 - Pf6N2E2_2180 0.24 -1.9 2,585,402 + Pf6N2E2_2180 0.26 -0.7 2,585,781 - Pf6N2E2_2180 0.63 -1.7 2,585,781 - Pf6N2E2_2180 0.63 -2.0 2,585,781 - Pf6N2E2_2180 0.63 -1.2 2,585,781 - Pf6N2E2_2180 0.63 -2.3 2,585,888 + Pf6N2E2_2180 0.73 -0.3 2,585,888 + +1.5 2,585,889 - Pf6N2E2_2180 0.74 -2.4 2,585,889 - Pf6N2E2_2180 0.74 -1.3 2,585,896 + -0.8 2,586,038 + Pf6N2E2_2180 0.88 -1.6 2,586,153 + -1.7 2,586,201 + +0.1 2,586,201 + +0.4 2,586,201 + -0.1 2,586,201 + -0.6 2,586,202 - -1.0 2,586,202 - -1.1 2,586,202 - -1.2 2,586,202 - -1.0 2,586,202 - +0.5 2,586,202 - -0.9 2,586,472 - +1.5 2,586,472 - Pf6N2E2_2181 0.24 -1.5 2,586,477 + Pf6N2E2_2181 0.25 -0.6 2,586,850 - Pf6N2E2_2181 0.61 -1.5
Or see this region's nucleotide sequence