Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2175 and Pf6N2E2_2176 are separated by 2 nucleotides Pf6N2E2_2176 and Pf6N2E2_2177 overlap by 4 nucleotides
Pf6N2E2_2175 - Transcriptional regulator, RpiR family, at 2,580,015 to 2,580,869
_2175
Pf6N2E2_2176 - Amidases related to nicotinamidase, at 2,580,872 to 2,581,516
_2176
Pf6N2E2_2177 - Glutamine synthetase (EC 6.3.1.2), putative, at 2,581,513 to 2,582,868
_2177
Position (kb)
2580
2581
2582 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2579.881 kb on + strand at 2579.881 kb on + strand at 2579.881 kb on + strand at 2579.882 kb on - strand at 2579.882 kb on - strand at 2579.923 kb on + strand at 2579.924 kb on - strand at 2579.924 kb on - strand at 2579.942 kb on - strand at 2579.942 kb on - strand at 2579.942 kb on - strand at 2579.951 kb on + strand at 2579.955 kb on + strand at 2579.955 kb on + strand at 2579.956 kb on - strand at 2579.956 kb on - strand at 2579.959 kb on + strand at 2579.960 kb on - strand at 2579.975 kb on + strand at 2579.976 kb on - strand at 2580.418 kb on + strand, within Pf6N2E2_2175 at 2580.418 kb on + strand, within Pf6N2E2_2175 at 2580.418 kb on + strand, within Pf6N2E2_2175 at 2580.459 kb on + strand, within Pf6N2E2_2175 at 2580.459 kb on + strand, within Pf6N2E2_2175 at 2580.660 kb on + strand, within Pf6N2E2_2175 at 2580.867 kb on + strand at 2580.867 kb on + strand at 2581.008 kb on - strand, within Pf6N2E2_2176 at 2581.277 kb on + strand, within Pf6N2E2_2176 at 2581.412 kb on + strand, within Pf6N2E2_2176 at 2581.413 kb on - strand, within Pf6N2E2_2176 at 2581.457 kb on + strand at 2581.633 kb on + strand at 2581.633 kb on + strand at 2581.634 kb on - strand at 2581.634 kb on - strand at 2581.634 kb on - strand at 2581.721 kb on - strand, within Pf6N2E2_2177 at 2581.807 kb on - strand, within Pf6N2E2_2177 at 2582.092 kb on + strand, within Pf6N2E2_2177 at 2582.151 kb on - strand, within Pf6N2E2_2177 at 2582.353 kb on + strand, within Pf6N2E2_2177 at 2582.398 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 2,579,881 + +1.5 2,579,881 + +0.7 2,579,881 + +0.0 2,579,882 - +0.0 2,579,882 - +0.1 2,579,923 + +2.5 2,579,924 - +1.5 2,579,924 - -0.5 2,579,942 - -0.2 2,579,942 - -0.0 2,579,942 - -1.5 2,579,951 + +0.8 2,579,955 + -0.3 2,579,955 + -0.0 2,579,956 - -1.5 2,579,956 - -0.1 2,579,959 + +0.1 2,579,960 - -1.0 2,579,975 + +1.0 2,579,976 - -0.7 2,580,418 + Pf6N2E2_2175 0.47 -0.3 2,580,418 + Pf6N2E2_2175 0.47 +1.8 2,580,418 + Pf6N2E2_2175 0.47 +0.0 2,580,459 + Pf6N2E2_2175 0.52 -0.0 2,580,459 + Pf6N2E2_2175 0.52 -0.2 2,580,660 + Pf6N2E2_2175 0.75 +0.4 2,580,867 + -1.8 2,580,867 + +0.1 2,581,008 - Pf6N2E2_2176 0.21 -0.8 2,581,277 + Pf6N2E2_2176 0.63 -1.5 2,581,412 + Pf6N2E2_2176 0.84 +0.5 2,581,413 - Pf6N2E2_2176 0.84 -2.8 2,581,457 + -1.2 2,581,633 + -0.5 2,581,633 + -1.2 2,581,634 - -0.8 2,581,634 - -1.4 2,581,634 - -0.3 2,581,721 - Pf6N2E2_2177 0.15 -1.5 2,581,807 - Pf6N2E2_2177 0.22 -1.2 2,582,092 + Pf6N2E2_2177 0.43 -1.3 2,582,151 - Pf6N2E2_2177 0.47 -0.7 2,582,353 + Pf6N2E2_2177 0.62 -0.5 2,582,398 + Pf6N2E2_2177 0.65 -0.3 2,582,434 + Pf6N2E2_2177 0.68 -0.5 2,582,434 + Pf6N2E2_2177 0.68 +0.0 2,582,434 + Pf6N2E2_2177 0.68 +0.0 2,582,435 - Pf6N2E2_2177 0.68 -1.0 2,582,435 - Pf6N2E2_2177 0.68 -0.8 2,582,435 - Pf6N2E2_2177 0.68 -1.4 2,582,435 - Pf6N2E2_2177 0.68 -1.7
Or see this region's nucleotide sequence