Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2032 and Pf6N2E2_2033 are separated by 8 nucleotides Pf6N2E2_2033 and Pf6N2E2_2034 are separated by 5 nucleotides Pf6N2E2_2034 and Pf6N2E2_2035 are separated by 67 nucleotides
Pf6N2E2_2032 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 2,420,802 to 2,421,824
_2032
Pf6N2E2_2033 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 2,421,833 to 2,423,425
_2033
Pf6N2E2_2034 - Methyltransferase, at 2,423,431 to 2,424,294
_2034
Pf6N2E2_2035 - epoxide hydrolase, at 2,424,362 to 2,425,366
_2035
Position (kb)
2421
2422
2423
2424 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2420.952 kb on + strand, within Pf6N2E2_2032 at 2420.953 kb on - strand, within Pf6N2E2_2032 at 2420.968 kb on - strand, within Pf6N2E2_2032 at 2421.045 kb on + strand, within Pf6N2E2_2032 at 2421.045 kb on + strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.113 kb on + strand, within Pf6N2E2_2032 at 2421.113 kb on + strand, within Pf6N2E2_2032 at 2421.114 kb on - strand, within Pf6N2E2_2032 at 2421.123 kb on + strand, within Pf6N2E2_2032 at 2421.123 kb on + strand, within Pf6N2E2_2032 at 2421.123 kb on + strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.233 kb on + strand, within Pf6N2E2_2032 at 2421.303 kb on + strand, within Pf6N2E2_2032 at 2421.304 kb on - strand, within Pf6N2E2_2032 at 2421.306 kb on + strand, within Pf6N2E2_2032 at 2421.307 kb on - strand, within Pf6N2E2_2032 at 2421.307 kb on - strand, within Pf6N2E2_2032 at 2421.372 kb on + strand, within Pf6N2E2_2032 at 2421.372 kb on + strand, within Pf6N2E2_2032 at 2421.373 kb on - strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.505 kb on - strand, within Pf6N2E2_2032 at 2421.594 kb on + strand, within Pf6N2E2_2032 at 2421.594 kb on + strand, within Pf6N2E2_2032 at 2421.594 kb on + strand, within Pf6N2E2_2032 at 2421.595 kb on - strand, within Pf6N2E2_2032 at 2421.865 kb on - strand at 2422.589 kb on + strand, within Pf6N2E2_2033 at 2422.589 kb on + strand, within Pf6N2E2_2033 at 2422.590 kb on - strand, within Pf6N2E2_2033 at 2422.721 kb on + strand, within Pf6N2E2_2033 at 2422.722 kb on - strand, within Pf6N2E2_2033 at 2422.741 kb on + strand, within Pf6N2E2_2033 at 2422.742 kb on - strand, within Pf6N2E2_2033 at 2423.249 kb on + strand, within Pf6N2E2_2033 at 2423.261 kb on + strand, within Pf6N2E2_2033 at 2423.261 kb on + strand, within Pf6N2E2_2033 at 2423.262 kb on - strand, within Pf6N2E2_2033 at 2423.262 kb on - strand, within Pf6N2E2_2033 at 2423.387 kb on + strand at 2423.387 kb on + strand at 2423.387 kb on + strand at 2423.388 kb on - strand at 2423.388 kb on - strand at 2423.389 kb on + strand at 2423.390 kb on - strand at 2423.567 kb on - strand, within Pf6N2E2_2034 at 2423.567 kb on - strand, within Pf6N2E2_2034 at 2423.889 kb on + strand, within Pf6N2E2_2034 at 2424.013 kb on - strand, within Pf6N2E2_2034 at 2424.275 kb on + strand at 2424.364 kb on - strand at 2424.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 2,420,952 + Pf6N2E2_2032 0.15 -0.7 2,420,953 - Pf6N2E2_2032 0.15 +2.9 2,420,968 - Pf6N2E2_2032 0.16 +0.2 2,421,045 + Pf6N2E2_2032 0.24 +0.2 2,421,045 + Pf6N2E2_2032 0.24 -0.0 2,421,046 - Pf6N2E2_2032 0.24 +0.2 2,421,046 - Pf6N2E2_2032 0.24 -1.0 2,421,046 - Pf6N2E2_2032 0.24 -0.5 2,421,046 - Pf6N2E2_2032 0.24 -0.4 2,421,046 - Pf6N2E2_2032 0.24 -0.7 2,421,113 + Pf6N2E2_2032 0.30 +0.2 2,421,113 + Pf6N2E2_2032 0.30 +0.2 2,421,114 - Pf6N2E2_2032 0.30 +0.5 2,421,123 + Pf6N2E2_2032 0.31 -1.0 2,421,123 + Pf6N2E2_2032 0.31 +1.1 2,421,123 + Pf6N2E2_2032 0.31 -0.4 2,421,124 - Pf6N2E2_2032 0.31 -0.1 2,421,124 - Pf6N2E2_2032 0.31 +0.4 2,421,124 - Pf6N2E2_2032 0.31 +0.2 2,421,124 - Pf6N2E2_2032 0.31 -1.3 2,421,233 + Pf6N2E2_2032 0.42 -0.1 2,421,303 + Pf6N2E2_2032 0.49 -0.0 2,421,304 - Pf6N2E2_2032 0.49 +0.4 2,421,306 + Pf6N2E2_2032 0.49 +0.0 2,421,307 - Pf6N2E2_2032 0.49 +0.1 2,421,307 - Pf6N2E2_2032 0.49 +0.2 2,421,372 + Pf6N2E2_2032 0.56 -0.5 2,421,372 + Pf6N2E2_2032 0.56 +0.3 2,421,373 - Pf6N2E2_2032 0.56 +0.9 2,421,504 + Pf6N2E2_2032 0.69 +3.1 2,421,504 + Pf6N2E2_2032 0.69 +0.9 2,421,504 + Pf6N2E2_2032 0.69 -0.7 2,421,504 + Pf6N2E2_2032 0.69 -0.1 2,421,505 - Pf6N2E2_2032 0.69 +0.3 2,421,594 + Pf6N2E2_2032 0.77 -0.5 2,421,594 + Pf6N2E2_2032 0.77 +0.7 2,421,594 + Pf6N2E2_2032 0.77 -0.8 2,421,595 - Pf6N2E2_2032 0.78 -0.3 2,421,865 - +1.7 2,422,589 + Pf6N2E2_2033 0.47 -2.3 2,422,589 + Pf6N2E2_2033 0.47 -1.9 2,422,590 - Pf6N2E2_2033 0.48 -0.4 2,422,721 + Pf6N2E2_2033 0.56 -0.4 2,422,722 - Pf6N2E2_2033 0.56 -0.7 2,422,741 + Pf6N2E2_2033 0.57 -2.9 2,422,742 - Pf6N2E2_2033 0.57 +1.4 2,423,249 + Pf6N2E2_2033 0.89 -0.2 2,423,261 + Pf6N2E2_2033 0.90 +1.3 2,423,261 + Pf6N2E2_2033 0.90 -2.2 2,423,262 - Pf6N2E2_2033 0.90 -0.5 2,423,262 - Pf6N2E2_2033 0.90 +1.0 2,423,387 + -0.3 2,423,387 + +0.4 2,423,387 + +0.7 2,423,388 - -0.4 2,423,388 - +0.1 2,423,389 + -0.1 2,423,390 - -0.5 2,423,567 - Pf6N2E2_2034 0.16 +0.3 2,423,567 - Pf6N2E2_2034 0.16 +1.3 2,423,889 + Pf6N2E2_2034 0.53 -0.5 2,424,013 - Pf6N2E2_2034 0.67 +0.2 2,424,275 + +0.5 2,424,364 - -0.6 2,424,364 - +1.5
Or see this region's nucleotide sequence