Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1884 and Pf6N2E2_1885 are separated by 666 nucleotides Pf6N2E2_1885 and Pf6N2E2_1886 are separated by 639 nucleotides Pf6N2E2_1886 and Pf6N2E2_1887 are separated by 17 nucleotides Pf6N2E2_1887 and Pf6N2E2_1888 overlap by 47 nucleotides
Pf6N2E2_1884 - oxidoreductase, short-chain dehydrogenase/reductase family, at 2,235,697 to 2,236,422
_1884
Pf6N2E2_1885 - Transcriptional regulator, AraC family, at 2,237,089 to 2,237,466
_1885
Pf6N2E2_1886 - hypothetical protein, at 2,238,106 to 2,238,237
_1886
Pf6N2E2_1887 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 2,238,255 to 2,238,368
_1887
Pf6N2E2_1888 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 2,238,322 to 2,239,461
_1888
Position (kb)
2237
2238 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2236.393 kb on + strand at 2236.394 kb on - strand at 2236.464 kb on - strand at 2236.464 kb on - strand at 2236.464 kb on - strand at 2236.485 kb on - strand at 2236.496 kb on - strand at 2236.496 kb on - strand at 2236.501 kb on + strand at 2236.501 kb on + strand at 2236.677 kb on + strand at 2236.677 kb on + strand at 2236.768 kb on + strand at 2236.769 kb on - strand at 2236.783 kb on + strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.784 kb on - strand at 2236.787 kb on + strand at 2236.788 kb on - strand at 2236.788 kb on - strand at 2236.815 kb on - strand at 2236.880 kb on + strand at 2236.881 kb on - strand at 2236.883 kb on + strand at 2236.884 kb on - strand at 2236.884 kb on - strand at 2236.934 kb on + strand at 2236.941 kb on - strand at 2236.941 kb on - strand at 2236.950 kb on - strand at 2236.950 kb on - strand at 2237.042 kb on + strand at 2237.060 kb on - strand at 2237.062 kb on - strand at 2237.062 kb on - strand at 2237.132 kb on + strand, within Pf6N2E2_1885 at 2237.132 kb on + strand, within Pf6N2E2_1885 at 2237.151 kb on - strand, within Pf6N2E2_1885 at 2237.270 kb on - strand, within Pf6N2E2_1885 at 2237.464 kb on + strand at 2237.570 kb on + strand at 2237.571 kb on - strand at 2237.571 kb on - strand at 2237.743 kb on + strand at 2237.744 kb on - strand at 2237.744 kb on - strand at 2237.772 kb on + strand at 2237.864 kb on + strand at 2237.865 kb on - strand at 2237.920 kb on + strand at 2237.921 kb on - strand at 2237.921 kb on - strand at 2238.026 kb on + strand at 2238.102 kb on - strand at 2238.102 kb on - strand at 2238.157 kb on - strand, within Pf6N2E2_1886 at 2238.157 kb on - strand, within Pf6N2E2_1886 at 2238.160 kb on - strand, within Pf6N2E2_1886 at 2238.236 kb on - strand at 2238.236 kb on - strand at 2238.364 kb on + strand at 2238.364 kb on + strand at 2238.364 kb on + strand at 2238.364 kb on + strand at 2238.365 kb on - strand at 2238.365 kb on - strand at 2238.412 kb on + strand at 2238.415 kb on + strand at 2238.449 kb on + strand, within Pf6N2E2_1888 at 2238.450 kb on - strand, within Pf6N2E2_1888 at 2238.450 kb on - strand, within Pf6N2E2_1888
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 2,236,393 + +0.5 2,236,394 - -0.3 2,236,464 - +1.5 2,236,464 - +3.5 2,236,464 - -0.2 2,236,485 - -1.2 2,236,496 - +1.5 2,236,496 - +1.9 2,236,501 + -0.4 2,236,501 + -0.2 2,236,677 + +0.1 2,236,677 + -0.5 2,236,768 + +0.1 2,236,769 - -0.4 2,236,783 + +0.2 2,236,784 - -0.2 2,236,784 - -0.0 2,236,784 - +1.5 2,236,784 - -0.9 2,236,784 - -1.6 2,236,787 + +0.7 2,236,788 - -2.0 2,236,788 - +1.5 2,236,815 - +0.0 2,236,880 + +1.5 2,236,881 - +0.7 2,236,883 + +0.3 2,236,884 - -0.4 2,236,884 - +0.8 2,236,934 + -0.5 2,236,941 - -0.6 2,236,941 - +1.5 2,236,950 - -0.0 2,236,950 - -0.1 2,237,042 + +1.5 2,237,060 - -1.8 2,237,062 - +0.4 2,237,062 - -1.6 2,237,132 + Pf6N2E2_1885 0.11 -2.0 2,237,132 + Pf6N2E2_1885 0.11 -1.1 2,237,151 - Pf6N2E2_1885 0.16 -2.3 2,237,270 - Pf6N2E2_1885 0.48 -0.7 2,237,464 + -0.3 2,237,570 + -1.6 2,237,571 - -1.5 2,237,571 - -0.8 2,237,743 + -0.7 2,237,744 - +1.0 2,237,744 - +1.5 2,237,772 + +0.2 2,237,864 + +1.5 2,237,865 - -0.2 2,237,920 + -0.6 2,237,921 - +0.2 2,237,921 - +0.4 2,238,026 + -0.4 2,238,102 - +0.9 2,238,102 - +0.5 2,238,157 - Pf6N2E2_1886 0.39 -0.5 2,238,157 - Pf6N2E2_1886 0.39 -0.3 2,238,160 - Pf6N2E2_1886 0.41 -1.0 2,238,236 - -0.4 2,238,236 - -0.1 2,238,364 + -0.1 2,238,364 + +0.3 2,238,364 + -0.1 2,238,364 + -0.1 2,238,365 - -0.9 2,238,365 - +0.1 2,238,412 + +0.4 2,238,415 + -1.3 2,238,449 + Pf6N2E2_1888 0.11 +0.5 2,238,450 - Pf6N2E2_1888 0.11 -1.9 2,238,450 - Pf6N2E2_1888 0.11 -1.5
Or see this region's nucleotide sequence