Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1812

Experiment: Nitrate reduction, replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1810 and Pf6N2E2_1811 are separated by 9 nucleotidesPf6N2E2_1811 and Pf6N2E2_1812 are separated by 31 nucleotidesPf6N2E2_1812 and Pf6N2E2_1813 overlap by 4 nucleotidesPf6N2E2_1813 and Pf6N2E2_1814 overlap by 4 nucleotides Pf6N2E2_1810 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 2,142,347 to 2,143,264 _1810 Pf6N2E2_1811 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 2,143,274 to 2,144,176 _1811 Pf6N2E2_1812 - peptide ABC transporter, ATP-binding protein, at 2,144,208 to 2,145,224 _1812 Pf6N2E2_1813 - ABC-type oligopeptide transport system, ATPase component, at 2,145,221 to 2,146,210 _1813 Pf6N2E2_1814 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 2,146,207 to 2,147,631 _1814 Position (kb) 2144 2145 2146Strain fitness (log2 ratio) -1 0 1 2 3at 2143.271 kb on + strandat 2143.272 kb on - strandat 2143.272 kb on - strandat 2143.307 kb on + strandat 2143.436 kb on + strand, within Pf6N2E2_1811at 2143.437 kb on - strand, within Pf6N2E2_1811at 2143.508 kb on + strand, within Pf6N2E2_1811at 2143.508 kb on + strand, within Pf6N2E2_1811at 2143.508 kb on + strand, within Pf6N2E2_1811at 2143.672 kb on + strand, within Pf6N2E2_1811at 2143.680 kb on - strand, within Pf6N2E2_1811at 2143.848 kb on - strand, within Pf6N2E2_1811at 2144.055 kb on - strand, within Pf6N2E2_1811at 2144.055 kb on - strand, within Pf6N2E2_1811at 2144.055 kb on - strand, within Pf6N2E2_1811at 2144.055 kb on - strand, within Pf6N2E2_1811at 2144.055 kb on - strand, within Pf6N2E2_1811at 2144.055 kb on - strand, within Pf6N2E2_1811at 2144.190 kb on + strandat 2144.190 kb on + strandat 2144.190 kb on + strandat 2144.191 kb on - strandat 2144.191 kb on - strandat 2144.191 kb on - strandat 2144.194 kb on - strandat 2144.194 kb on - strandat 2144.194 kb on - strandat 2144.215 kb on + strandat 2144.216 kb on - strandat 2144.539 kb on - strand, within Pf6N2E2_1812at 2144.539 kb on - strand, within Pf6N2E2_1812at 2144.904 kb on + strand, within Pf6N2E2_1812at 2144.905 kb on - strand, within Pf6N2E2_1812at 2144.905 kb on - strand, within Pf6N2E2_1812at 2144.967 kb on + strand, within Pf6N2E2_1812at 2144.990 kb on + strand, within Pf6N2E2_1812at 2145.403 kb on - strand, within Pf6N2E2_1813at 2145.518 kb on + strand, within Pf6N2E2_1813at 2145.518 kb on + strand, within Pf6N2E2_1813at 2145.836 kb on + strand, within Pf6N2E2_1813at 2145.837 kb on - strand, within Pf6N2E2_1813at 2145.837 kb on - strand, within Pf6N2E2_1813at 2145.837 kb on - strand, within Pf6N2E2_1813at 2145.837 kb on - strand, within Pf6N2E2_1813at 2145.841 kb on - strand, within Pf6N2E2_1813at 2145.900 kb on - strand, within Pf6N2E2_1813at 2145.940 kb on - strand, within Pf6N2E2_1813at 2145.962 kb on + strand, within Pf6N2E2_1813at 2145.963 kb on - strand, within Pf6N2E2_1813

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B
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2,143,271 + -1.0
2,143,272 - -0.0
2,143,272 - -1.0
2,143,307 + -0.7
2,143,436 + Pf6N2E2_1811 0.18 +0.2
2,143,437 - Pf6N2E2_1811 0.18 -0.7
2,143,508 + Pf6N2E2_1811 0.26 -0.3
2,143,508 + Pf6N2E2_1811 0.26 +0.5
2,143,508 + Pf6N2E2_1811 0.26 -0.9
2,143,672 + Pf6N2E2_1811 0.44 -1.1
2,143,680 - Pf6N2E2_1811 0.45 -0.0
2,143,848 - Pf6N2E2_1811 0.64 +1.2
2,144,055 - Pf6N2E2_1811 0.86 +1.5
2,144,055 - Pf6N2E2_1811 0.86 -0.9
2,144,055 - Pf6N2E2_1811 0.86 +2.1
2,144,055 - Pf6N2E2_1811 0.86 +0.2
2,144,055 - Pf6N2E2_1811 0.86 -0.5
2,144,055 - Pf6N2E2_1811 0.86 -0.1
2,144,190 + -0.1
2,144,190 + -0.9
2,144,190 + -0.5
2,144,191 - +0.5
2,144,191 - -0.7
2,144,191 - -0.6
2,144,194 - +0.4
2,144,194 - +0.7
2,144,194 - -0.5
2,144,215 + -0.7
2,144,216 - +0.1
2,144,539 - Pf6N2E2_1812 0.33 -1.0
2,144,539 - Pf6N2E2_1812 0.33 -1.3
2,144,904 + Pf6N2E2_1812 0.68 +1.5
2,144,905 - Pf6N2E2_1812 0.69 +1.5
2,144,905 - Pf6N2E2_1812 0.69 -1.2
2,144,967 + Pf6N2E2_1812 0.75 -0.2
2,144,990 + Pf6N2E2_1812 0.77 -1.5
2,145,403 - Pf6N2E2_1813 0.18 +0.9
2,145,518 + Pf6N2E2_1813 0.30 +1.5
2,145,518 + Pf6N2E2_1813 0.30 +0.0
2,145,836 + Pf6N2E2_1813 0.62 -0.5
2,145,837 - Pf6N2E2_1813 0.62 +0.5
2,145,837 - Pf6N2E2_1813 0.62 -0.4
2,145,837 - Pf6N2E2_1813 0.62 +0.1
2,145,837 - Pf6N2E2_1813 0.62 +1.0
2,145,841 - Pf6N2E2_1813 0.63 -0.7
2,145,900 - Pf6N2E2_1813 0.69 +2.8
2,145,940 - Pf6N2E2_1813 0.73 +0.3
2,145,962 + Pf6N2E2_1813 0.75 +1.0
2,145,963 - Pf6N2E2_1813 0.75 +0.8

Or see this region's nucleotide sequence