Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1810 and Pf6N2E2_1811 are separated by 9 nucleotides Pf6N2E2_1811 and Pf6N2E2_1812 are separated by 31 nucleotides Pf6N2E2_1812 and Pf6N2E2_1813 overlap by 4 nucleotides Pf6N2E2_1813 and Pf6N2E2_1814 overlap by 4 nucleotides
Pf6N2E2_1810 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 2,142,347 to 2,143,264
_1810
Pf6N2E2_1811 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 2,143,274 to 2,144,176
_1811
Pf6N2E2_1812 - peptide ABC transporter, ATP-binding protein, at 2,144,208 to 2,145,224
_1812
Pf6N2E2_1813 - ABC-type oligopeptide transport system, ATPase component, at 2,145,221 to 2,146,210
_1813
Pf6N2E2_1814 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 2,146,207 to 2,147,631
_1814
Position (kb)
2144
2145
2146 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2143.271 kb on + strand at 2143.272 kb on - strand at 2143.272 kb on - strand at 2143.307 kb on + strand at 2143.436 kb on + strand, within Pf6N2E2_1811 at 2143.437 kb on - strand, within Pf6N2E2_1811 at 2143.508 kb on + strand, within Pf6N2E2_1811 at 2143.508 kb on + strand, within Pf6N2E2_1811 at 2143.508 kb on + strand, within Pf6N2E2_1811 at 2143.672 kb on + strand, within Pf6N2E2_1811 at 2143.680 kb on - strand, within Pf6N2E2_1811 at 2143.848 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.190 kb on + strand at 2144.190 kb on + strand at 2144.190 kb on + strand at 2144.191 kb on - strand at 2144.191 kb on - strand at 2144.191 kb on - strand at 2144.194 kb on - strand at 2144.194 kb on - strand at 2144.194 kb on - strand at 2144.215 kb on + strand at 2144.216 kb on - strand at 2144.539 kb on - strand, within Pf6N2E2_1812 at 2144.539 kb on - strand, within Pf6N2E2_1812 at 2144.904 kb on + strand, within Pf6N2E2_1812 at 2144.905 kb on - strand, within Pf6N2E2_1812 at 2144.905 kb on - strand, within Pf6N2E2_1812 at 2144.967 kb on + strand, within Pf6N2E2_1812 at 2144.990 kb on + strand, within Pf6N2E2_1812 at 2145.403 kb on - strand, within Pf6N2E2_1813 at 2145.518 kb on + strand, within Pf6N2E2_1813 at 2145.518 kb on + strand, within Pf6N2E2_1813 at 2145.836 kb on + strand, within Pf6N2E2_1813 at 2145.837 kb on - strand, within Pf6N2E2_1813 at 2145.837 kb on - strand, within Pf6N2E2_1813 at 2145.837 kb on - strand, within Pf6N2E2_1813 at 2145.837 kb on - strand, within Pf6N2E2_1813 at 2145.841 kb on - strand, within Pf6N2E2_1813 at 2145.900 kb on - strand, within Pf6N2E2_1813 at 2145.940 kb on - strand, within Pf6N2E2_1813 at 2145.962 kb on + strand, within Pf6N2E2_1813 at 2145.963 kb on - strand, within Pf6N2E2_1813
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 2,143,271 + -1.0 2,143,272 - -0.0 2,143,272 - -1.0 2,143,307 + -0.7 2,143,436 + Pf6N2E2_1811 0.18 +0.2 2,143,437 - Pf6N2E2_1811 0.18 -0.7 2,143,508 + Pf6N2E2_1811 0.26 -0.3 2,143,508 + Pf6N2E2_1811 0.26 +0.5 2,143,508 + Pf6N2E2_1811 0.26 -0.9 2,143,672 + Pf6N2E2_1811 0.44 -1.1 2,143,680 - Pf6N2E2_1811 0.45 -0.0 2,143,848 - Pf6N2E2_1811 0.64 +1.2 2,144,055 - Pf6N2E2_1811 0.86 +1.5 2,144,055 - Pf6N2E2_1811 0.86 -0.9 2,144,055 - Pf6N2E2_1811 0.86 +2.1 2,144,055 - Pf6N2E2_1811 0.86 +0.2 2,144,055 - Pf6N2E2_1811 0.86 -0.5 2,144,055 - Pf6N2E2_1811 0.86 -0.1 2,144,190 + -0.1 2,144,190 + -0.9 2,144,190 + -0.5 2,144,191 - +0.5 2,144,191 - -0.7 2,144,191 - -0.6 2,144,194 - +0.4 2,144,194 - +0.7 2,144,194 - -0.5 2,144,215 + -0.7 2,144,216 - +0.1 2,144,539 - Pf6N2E2_1812 0.33 -1.0 2,144,539 - Pf6N2E2_1812 0.33 -1.3 2,144,904 + Pf6N2E2_1812 0.68 +1.5 2,144,905 - Pf6N2E2_1812 0.69 +1.5 2,144,905 - Pf6N2E2_1812 0.69 -1.2 2,144,967 + Pf6N2E2_1812 0.75 -0.2 2,144,990 + Pf6N2E2_1812 0.77 -1.5 2,145,403 - Pf6N2E2_1813 0.18 +0.9 2,145,518 + Pf6N2E2_1813 0.30 +1.5 2,145,518 + Pf6N2E2_1813 0.30 +0.0 2,145,836 + Pf6N2E2_1813 0.62 -0.5 2,145,837 - Pf6N2E2_1813 0.62 +0.5 2,145,837 - Pf6N2E2_1813 0.62 -0.4 2,145,837 - Pf6N2E2_1813 0.62 +0.1 2,145,837 - Pf6N2E2_1813 0.62 +1.0 2,145,841 - Pf6N2E2_1813 0.63 -0.7 2,145,900 - Pf6N2E2_1813 0.69 +2.8 2,145,940 - Pf6N2E2_1813 0.73 +0.3 2,145,962 + Pf6N2E2_1813 0.75 +1.0 2,145,963 - Pf6N2E2_1813 0.75 +0.8
Or see this region's nucleotide sequence