Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1410 and Pf6N2E2_1411 overlap by 4 nucleotides Pf6N2E2_1411 and Pf6N2E2_1412 overlap by 15 nucleotides Pf6N2E2_1412 and Pf6N2E2_1413 are separated by 53 nucleotides Pf6N2E2_1413 and Pf6N2E2_1414 are separated by 158 nucleotides
Pf6N2E2_1410 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 1,655,263 to 1,656,651
_1410
Pf6N2E2_1411 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 1,656,648 to 1,657,742
_1411
Pf6N2E2_1412 - hypothetical protein, at 1,657,728 to 1,658,063
_1412
Pf6N2E2_1413 - putative membrane protein, at 1,658,117 to 1,658,560
_1413
Pf6N2E2_1414 - hypothetical protein, at 1,658,719 to 1,658,901
_1414
Position (kb)
1656
1657
1658 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1655.697 kb on - strand, within Pf6N2E2_1410 at 1655.783 kb on + strand, within Pf6N2E2_1410 at 1655.783 kb on + strand, within Pf6N2E2_1410 at 1655.783 kb on + strand, within Pf6N2E2_1410 at 1655.783 kb on + strand, within Pf6N2E2_1410 at 1655.784 kb on - strand, within Pf6N2E2_1410 at 1655.784 kb on - strand, within Pf6N2E2_1410 at 1655.784 kb on - strand, within Pf6N2E2_1410 at 1655.909 kb on + strand, within Pf6N2E2_1410 at 1655.909 kb on + strand, within Pf6N2E2_1410 at 1655.909 kb on + strand, within Pf6N2E2_1410 at 1655.916 kb on - strand, within Pf6N2E2_1410 at 1655.916 kb on - strand, within Pf6N2E2_1410 at 1656.000 kb on - strand, within Pf6N2E2_1410 at 1656.000 kb on - strand, within Pf6N2E2_1410 at 1656.000 kb on - strand, within Pf6N2E2_1410 at 1656.290 kb on + strand, within Pf6N2E2_1410 at 1656.291 kb on - strand, within Pf6N2E2_1410 at 1656.443 kb on + strand, within Pf6N2E2_1410 at 1656.476 kb on + strand, within Pf6N2E2_1410 at 1656.476 kb on + strand, within Pf6N2E2_1410 at 1656.476 kb on + strand, within Pf6N2E2_1410 at 1656.477 kb on - strand, within Pf6N2E2_1410 at 1656.477 kb on - strand, within Pf6N2E2_1410 at 1656.477 kb on - strand, within Pf6N2E2_1410 at 1656.477 kb on - strand, within Pf6N2E2_1410 at 1656.578 kb on + strand at 1656.608 kb on + strand at 1656.988 kb on + strand, within Pf6N2E2_1411 at 1656.989 kb on - strand, within Pf6N2E2_1411 at 1657.014 kb on + strand, within Pf6N2E2_1411 at 1657.014 kb on + strand, within Pf6N2E2_1411 at 1657.014 kb on + strand, within Pf6N2E2_1411 at 1657.015 kb on - strand at 1657.015 kb on - strand, within Pf6N2E2_1411 at 1657.015 kb on - strand, within Pf6N2E2_1411 at 1657.015 kb on - strand, within Pf6N2E2_1411 at 1657.019 kb on - strand, within Pf6N2E2_1411 at 1657.019 kb on - strand, within Pf6N2E2_1411 at 1657.019 kb on - strand, within Pf6N2E2_1411 at 1657.037 kb on + strand, within Pf6N2E2_1411 at 1657.086 kb on - strand, within Pf6N2E2_1411 at 1657.174 kb on + strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.300 kb on + strand, within Pf6N2E2_1411 at 1657.301 kb on - strand, within Pf6N2E2_1411 at 1657.615 kb on + strand, within Pf6N2E2_1411 at 1657.616 kb on - strand, within Pf6N2E2_1411 at 1657.616 kb on - strand, within Pf6N2E2_1411 at 1657.753 kb on + strand at 1657.754 kb on - strand at 1657.754 kb on - strand at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.941 kb on - strand, within Pf6N2E2_1412 at 1658.130 kb on + strand at 1658.130 kb on + strand at 1658.130 kb on + strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.683 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 1,655,697 - Pf6N2E2_1410 0.31 -1.1 1,655,783 + Pf6N2E2_1410 0.37 -0.8 1,655,783 + Pf6N2E2_1410 0.37 +0.1 1,655,783 + Pf6N2E2_1410 0.37 -1.6 1,655,783 + Pf6N2E2_1410 0.37 -0.8 1,655,784 - Pf6N2E2_1410 0.38 +0.5 1,655,784 - Pf6N2E2_1410 0.38 +0.2 1,655,784 - Pf6N2E2_1410 0.38 -1.0 1,655,909 + Pf6N2E2_1410 0.47 -1.5 1,655,909 + Pf6N2E2_1410 0.47 +0.4 1,655,909 + Pf6N2E2_1410 0.47 -0.0 1,655,916 - Pf6N2E2_1410 0.47 -1.0 1,655,916 - Pf6N2E2_1410 0.47 -0.3 1,656,000 - Pf6N2E2_1410 0.53 +1.6 1,656,000 - Pf6N2E2_1410 0.53 +1.6 1,656,000 - Pf6N2E2_1410 0.53 +0.7 1,656,290 + Pf6N2E2_1410 0.74 -1.3 1,656,291 - Pf6N2E2_1410 0.74 +0.3 1,656,443 + Pf6N2E2_1410 0.85 +0.2 1,656,476 + Pf6N2E2_1410 0.87 +1.9 1,656,476 + Pf6N2E2_1410 0.87 -0.3 1,656,476 + Pf6N2E2_1410 0.87 -1.2 1,656,477 - Pf6N2E2_1410 0.87 +1.1 1,656,477 - Pf6N2E2_1410 0.87 -0.0 1,656,477 - Pf6N2E2_1410 0.87 +1.6 1,656,477 - Pf6N2E2_1410 0.87 +0.3 1,656,578 + +0.0 1,656,608 + +1.6 1,656,988 + Pf6N2E2_1411 0.31 -2.7 1,656,989 - Pf6N2E2_1411 0.31 +1.6 1,657,014 + Pf6N2E2_1411 0.33 -2.8 1,657,014 + Pf6N2E2_1411 0.33 -3.2 1,657,014 + Pf6N2E2_1411 0.33 +1.6 1,657,015 - +1.6 1,657,015 - Pf6N2E2_1411 0.34 -1.2 1,657,015 - Pf6N2E2_1411 0.34 -1.5 1,657,015 - Pf6N2E2_1411 0.34 -0.4 1,657,019 - Pf6N2E2_1411 0.34 -1.1 1,657,019 - Pf6N2E2_1411 0.34 -1.9 1,657,019 - Pf6N2E2_1411 0.34 -2.0 1,657,037 + Pf6N2E2_1411 0.36 -3.6 1,657,086 - Pf6N2E2_1411 0.40 +1.6 1,657,174 + Pf6N2E2_1411 0.48 -3.1 1,657,175 - Pf6N2E2_1411 0.48 -2.5 1,657,175 - Pf6N2E2_1411 0.48 +1.6 1,657,175 - Pf6N2E2_1411 0.48 -1.9 1,657,175 - Pf6N2E2_1411 0.48 -1.4 1,657,175 - Pf6N2E2_1411 0.48 -0.4 1,657,175 - Pf6N2E2_1411 0.48 -2.2 1,657,175 - Pf6N2E2_1411 0.48 +0.6 1,657,300 + Pf6N2E2_1411 0.60 -2.0 1,657,301 - Pf6N2E2_1411 0.60 -1.8 1,657,615 + Pf6N2E2_1411 0.88 +1.6 1,657,616 - Pf6N2E2_1411 0.88 -0.9 1,657,616 - Pf6N2E2_1411 0.88 -2.6 1,657,753 + -1.2 1,657,754 - -1.9 1,657,754 - -2.4 1,657,935 - Pf6N2E2_1412 0.62 -1.7 1,657,935 - Pf6N2E2_1412 0.62 -0.0 1,657,935 - Pf6N2E2_1412 0.62 -0.3 1,657,935 - Pf6N2E2_1412 0.62 -1.1 1,657,941 - Pf6N2E2_1412 0.63 +0.2 1,658,130 + +0.3 1,658,130 + +0.0 1,658,130 + -0.1 1,658,131 - -0.2 1,658,131 - -0.2 1,658,131 - +1.2 1,658,131 - +0.2 1,658,683 - +0.9
Or see this region's nucleotide sequence