Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1042 and Pf6N2E2_1043 are separated by 118 nucleotides Pf6N2E2_1043 and Pf6N2E2_1044 are separated by 176 nucleotides Pf6N2E2_1044 and Pf6N2E2_1045 are separated by 516 nucleotides
Pf6N2E2_1042 - Nitrate/nitrite transporter, at 1,216,292 to 1,217,611
_1042
Pf6N2E2_1043 - Transcriptional regulator, IclR family, at 1,217,730 to 1,218,494
_1043
Pf6N2E2_1044 - hypothetical protein, at 1,218,671 to 1,218,862
_1044
Pf6N2E2_1045 - hypothetical protein, at 1,219,379 to 1,219,516
_1045
Position (kb)
1217
1218
1219 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1217.108 kb on + strand, within Pf6N2E2_1042 at 1217.192 kb on + strand, within Pf6N2E2_1042 at 1217.390 kb on + strand, within Pf6N2E2_1042 at 1217.390 kb on + strand, within Pf6N2E2_1042 at 1217.390 kb on + strand, within Pf6N2E2_1042 at 1217.391 kb on - strand, within Pf6N2E2_1042 at 1217.412 kb on + strand, within Pf6N2E2_1042 at 1217.486 kb on + strand at 1217.510 kb on + strand at 1217.510 kb on + strand at 1217.510 kb on + strand at 1217.511 kb on - strand at 1217.656 kb on + strand at 1217.710 kb on + strand at 1217.710 kb on + strand at 1217.710 kb on + strand at 1217.729 kb on + strand at 1217.730 kb on - strand at 1217.730 kb on - strand at 1217.883 kb on + strand, within Pf6N2E2_1043 at 1218.081 kb on + strand, within Pf6N2E2_1043 at 1218.081 kb on + strand, within Pf6N2E2_1043 at 1218.081 kb on + strand, within Pf6N2E2_1043 at 1218.082 kb on - strand, within Pf6N2E2_1043 at 1218.082 kb on - strand, within Pf6N2E2_1043 at 1218.320 kb on + strand, within Pf6N2E2_1043 at 1218.321 kb on - strand, within Pf6N2E2_1043 at 1218.387 kb on - strand, within Pf6N2E2_1043 at 1218.534 kb on + strand at 1218.534 kb on + strand at 1218.541 kb on - strand at 1218.541 kb on - strand at 1218.558 kb on + strand at 1218.559 kb on - strand at 1218.565 kb on + strand at 1218.566 kb on - strand at 1218.566 kb on - strand at 1218.566 kb on - strand at 1218.629 kb on + strand at 1218.629 kb on + strand at 1218.629 kb on + strand at 1218.633 kb on + strand at 1218.634 kb on - strand at 1218.673 kb on - strand at 1218.707 kb on + strand, within Pf6N2E2_1044 at 1218.708 kb on - strand, within Pf6N2E2_1044 at 1218.708 kb on - strand, within Pf6N2E2_1044 at 1218.708 kb on - strand, within Pf6N2E2_1044 at 1218.710 kb on - strand, within Pf6N2E2_1044 at 1218.808 kb on + strand, within Pf6N2E2_1044 at 1218.809 kb on - strand, within Pf6N2E2_1044 at 1218.868 kb on + strand at 1218.868 kb on + strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.889 kb on - strand at 1218.950 kb on + strand at 1218.950 kb on + strand at 1218.950 kb on + strand at 1218.950 kb on + strand at 1218.950 kb on + strand at 1218.951 kb on - strand at 1218.951 kb on - strand at 1218.951 kb on - strand at 1219.027 kb on + strand at 1219.027 kb on + strand at 1219.179 kb on + strand at 1219.329 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 1,217,108 + Pf6N2E2_1042 0.62 -0.8 1,217,192 + Pf6N2E2_1042 0.68 +1.2 1,217,390 + Pf6N2E2_1042 0.83 +0.6 1,217,390 + Pf6N2E2_1042 0.83 -0.5 1,217,390 + Pf6N2E2_1042 0.83 +1.0 1,217,391 - Pf6N2E2_1042 0.83 +0.3 1,217,412 + Pf6N2E2_1042 0.85 -0.4 1,217,486 + -1.0 1,217,510 + +0.7 1,217,510 + -2.5 1,217,510 + +0.3 1,217,511 - -0.7 1,217,656 + +0.4 1,217,710 + -0.5 1,217,710 + -0.0 1,217,710 + -1.4 1,217,729 + -1.7 1,217,730 - +1.1 1,217,730 - -0.0 1,217,883 + Pf6N2E2_1043 0.20 -0.4 1,218,081 + Pf6N2E2_1043 0.46 -1.0 1,218,081 + Pf6N2E2_1043 0.46 -0.6 1,218,081 + Pf6N2E2_1043 0.46 -0.6 1,218,082 - Pf6N2E2_1043 0.46 +1.6 1,218,082 - Pf6N2E2_1043 0.46 -0.9 1,218,320 + Pf6N2E2_1043 0.77 +1.6 1,218,321 - Pf6N2E2_1043 0.77 +0.8 1,218,387 - Pf6N2E2_1043 0.86 -2.7 1,218,534 + -0.6 1,218,534 + +0.1 1,218,541 - +1.6 1,218,541 - -1.0 1,218,558 + +0.3 1,218,559 - -1.0 1,218,565 + -0.2 1,218,566 - -0.8 1,218,566 - +1.6 1,218,566 - -0.0 1,218,629 + +1.6 1,218,629 + -1.1 1,218,629 + -0.1 1,218,633 + -1.2 1,218,634 - -1.0 1,218,673 - +0.1 1,218,707 + Pf6N2E2_1044 0.19 +0.6 1,218,708 - Pf6N2E2_1044 0.19 +0.0 1,218,708 - Pf6N2E2_1044 0.19 -1.5 1,218,708 - Pf6N2E2_1044 0.19 -1.4 1,218,710 - Pf6N2E2_1044 0.20 -0.0 1,218,808 + Pf6N2E2_1044 0.71 +1.8 1,218,809 - Pf6N2E2_1044 0.72 +0.3 1,218,868 + +0.7 1,218,868 + +1.4 1,218,869 - +1.6 1,218,869 - -0.3 1,218,869 - +0.4 1,218,869 - -0.9 1,218,869 - +1.6 1,218,869 - +0.1 1,218,869 - +0.6 1,218,869 - +0.4 1,218,869 - +1.6 1,218,869 - -0.3 1,218,869 - -0.2 1,218,870 + -1.2 1,218,870 + -1.0 1,218,870 + +1.6 1,218,870 + -0.0 1,218,870 + -1.0 1,218,870 + +0.1 1,218,870 + +1.6 1,218,871 - -0.6 1,218,871 - +0.4 1,218,871 - +0.6 1,218,871 - -0.7 1,218,871 - +1.6 1,218,871 - -0.4 1,218,871 - +0.6 1,218,871 - +0.3 1,218,871 - +0.4 1,218,889 - +1.6 1,218,950 + -0.3 1,218,950 + -0.7 1,218,950 + +1.6 1,218,950 + +0.2 1,218,950 + +0.6 1,218,951 - +0.2 1,218,951 - -1.2 1,218,951 - +1.6 1,219,027 + -0.7 1,219,027 + +1.6 1,219,179 + -0.3 1,219,329 - -0.1
Or see this region's nucleotide sequence