Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_862 and Pf6N2E2_863 are separated by 287 nucleotides Pf6N2E2_863 and Pf6N2E2_864 overlap by 4 nucleotides Pf6N2E2_864 and Pf6N2E2_865 are separated by 3 nucleotides
Pf6N2E2_862 - Fumarylacetoacetate hydrolase family protein, at 1,010,456 to 1,011,634
_862
Pf6N2E2_863 - Transcriptional regulator, IclR family, at 1,011,922 to 1,012,728
_863
Pf6N2E2_864 - 2-keto-4-pentenoate hydratase (EC 4.2.1.80), at 1,012,725 to 1,013,525
_864
Pf6N2E2_865 - Fumarylacetoacetate hydrolase family protein, at 1,013,529 to 1,014,422
_865
Position (kb)
1011
1012
1013 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1010.935 kb on + strand, within Pf6N2E2_862 at 1010.959 kb on + strand, within Pf6N2E2_862 at 1010.959 kb on + strand, within Pf6N2E2_862 at 1010.960 kb on - strand, within Pf6N2E2_862 at 1010.960 kb on - strand, within Pf6N2E2_862 at 1010.972 kb on + strand, within Pf6N2E2_862 at 1011.493 kb on + strand, within Pf6N2E2_862 at 1011.493 kb on + strand, within Pf6N2E2_862 at 1011.493 kb on + strand, within Pf6N2E2_862 at 1011.494 kb on - strand, within Pf6N2E2_862 at 1011.557 kb on + strand at 1011.557 kb on + strand at 1011.557 kb on + strand at 1011.557 kb on + strand at 1011.558 kb on - strand at 1011.632 kb on + strand at 1011.632 kb on + strand at 1011.632 kb on + strand at 1011.632 kb on + strand at 1011.633 kb on - strand at 1011.636 kb on + strand at 1011.637 kb on - strand at 1011.637 kb on - strand at 1011.637 kb on - strand at 1011.702 kb on + strand at 1011.703 kb on - strand at 1011.738 kb on + strand at 1011.738 kb on + strand at 1011.829 kb on - strand at 1011.829 kb on - strand at 1011.829 kb on - strand at 1011.829 kb on - strand at 1011.831 kb on + strand at 1011.832 kb on - strand at 1011.832 kb on - strand at 1011.832 kb on - strand at 1011.907 kb on + strand at 1011.907 kb on + strand at 1011.907 kb on + strand at 1011.907 kb on + strand at 1011.908 kb on - strand at 1011.908 kb on - strand at 1011.908 kb on - strand at 1011.908 kb on - strand at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.297 kb on + strand, within Pf6N2E2_863 at 1012.504 kb on + strand, within Pf6N2E2_863 at 1012.636 kb on + strand, within Pf6N2E2_863 at 1012.636 kb on + strand, within Pf6N2E2_863 at 1012.724 kb on + strand at 1012.724 kb on + strand at 1012.725 kb on - strand at 1012.892 kb on - strand, within Pf6N2E2_864 at 1012.892 kb on - strand, within Pf6N2E2_864 at 1012.892 kb on - strand, within Pf6N2E2_864 at 1012.965 kb on + strand, within Pf6N2E2_864 at 1012.965 kb on + strand, within Pf6N2E2_864 at 1012.966 kb on - strand, within Pf6N2E2_864 at 1012.982 kb on - strand, within Pf6N2E2_864 at 1012.982 kb on - strand, within Pf6N2E2_864 at 1012.982 kb on - strand, within Pf6N2E2_864 at 1013.085 kb on + strand, within Pf6N2E2_864 at 1013.086 kb on - strand, within Pf6N2E2_864 at 1013.155 kb on - strand, within Pf6N2E2_864 at 1013.156 kb on + strand, within Pf6N2E2_864 at 1013.156 kb on + strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.204 kb on - strand, within Pf6N2E2_864 at 1013.222 kb on - strand, within Pf6N2E2_864 at 1013.379 kb on - strand, within Pf6N2E2_864 at 1013.427 kb on + strand, within Pf6N2E2_864 at 1013.427 kb on + strand, within Pf6N2E2_864 at 1013.427 kb on + strand, within Pf6N2E2_864 at 1013.428 kb on - strand, within Pf6N2E2_864 at 1013.537 kb on - strand at 1013.537 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 1,010,935 + Pf6N2E2_862 0.41 +1.5 1,010,959 + Pf6N2E2_862 0.43 +1.3 1,010,959 + Pf6N2E2_862 0.43 -0.5 1,010,960 - Pf6N2E2_862 0.43 -0.7 1,010,960 - Pf6N2E2_862 0.43 +2.5 1,010,972 + Pf6N2E2_862 0.44 +0.2 1,011,493 + Pf6N2E2_862 0.88 -1.5 1,011,493 + Pf6N2E2_862 0.88 -0.6 1,011,493 + Pf6N2E2_862 0.88 +0.2 1,011,494 - Pf6N2E2_862 0.88 +0.3 1,011,557 + -0.4 1,011,557 + -0.6 1,011,557 + -0.5 1,011,557 + -0.4 1,011,558 - +0.5 1,011,632 + -0.4 1,011,632 + +0.1 1,011,632 + -0.5 1,011,632 + -0.4 1,011,633 - +0.5 1,011,636 + +0.3 1,011,637 - -0.5 1,011,637 - -0.4 1,011,637 - +0.5 1,011,702 + +0.2 1,011,703 - +0.2 1,011,738 + -0.3 1,011,738 + +0.0 1,011,829 - -1.6 1,011,829 - +0.2 1,011,829 - -1.5 1,011,829 - +0.5 1,011,831 + +0.8 1,011,832 - -0.2 1,011,832 - -0.7 1,011,832 - -0.0 1,011,907 + +0.2 1,011,907 + +0.4 1,011,907 + -0.8 1,011,907 + +1.5 1,011,908 - -0.7 1,011,908 - +1.3 1,011,908 - -0.5 1,011,908 - +1.5 1,012,151 + Pf6N2E2_863 0.28 -1.3 1,012,151 + Pf6N2E2_863 0.28 -0.6 1,012,151 + Pf6N2E2_863 0.28 -0.3 1,012,151 + Pf6N2E2_863 0.28 -0.2 1,012,151 + Pf6N2E2_863 0.28 +1.5 1,012,293 + Pf6N2E2_863 0.46 -1.6 1,012,293 + Pf6N2E2_863 0.46 -2.0 1,012,293 + Pf6N2E2_863 0.46 -1.2 1,012,293 + Pf6N2E2_863 0.46 -0.4 1,012,293 + Pf6N2E2_863 0.46 -2.4 1,012,293 + Pf6N2E2_863 0.46 -1.8 1,012,293 + Pf6N2E2_863 0.46 -2.7 1,012,293 + Pf6N2E2_863 0.46 -3.5 1,012,293 + Pf6N2E2_863 0.46 -1.3 1,012,293 + Pf6N2E2_863 0.46 -0.2 1,012,293 + Pf6N2E2_863 0.46 -0.8 1,012,293 + Pf6N2E2_863 0.46 -0.9 1,012,297 + Pf6N2E2_863 0.46 -0.9 1,012,504 + Pf6N2E2_863 0.72 -0.0 1,012,636 + Pf6N2E2_863 0.88 -0.4 1,012,636 + Pf6N2E2_863 0.88 -0.1 1,012,724 + -1.6 1,012,724 + +0.5 1,012,725 - -0.7 1,012,892 - Pf6N2E2_864 0.21 -0.1 1,012,892 - Pf6N2E2_864 0.21 +0.2 1,012,892 - Pf6N2E2_864 0.21 +0.1 1,012,965 + Pf6N2E2_864 0.30 -0.4 1,012,965 + Pf6N2E2_864 0.30 +1.5 1,012,966 - Pf6N2E2_864 0.30 -0.3 1,012,982 - Pf6N2E2_864 0.32 -0.7 1,012,982 - Pf6N2E2_864 0.32 +0.5 1,012,982 - Pf6N2E2_864 0.32 -0.9 1,013,085 + Pf6N2E2_864 0.45 +0.4 1,013,086 - Pf6N2E2_864 0.45 +1.2 1,013,155 - Pf6N2E2_864 0.54 +1.5 1,013,156 + Pf6N2E2_864 0.54 +0.4 1,013,156 + Pf6N2E2_864 0.54 -0.1 1,013,157 - Pf6N2E2_864 0.54 +0.9 1,013,157 - Pf6N2E2_864 0.54 -1.1 1,013,157 - Pf6N2E2_864 0.54 -1.2 1,013,157 - Pf6N2E2_864 0.54 -0.9 1,013,204 - Pf6N2E2_864 0.60 -0.5 1,013,222 - Pf6N2E2_864 0.62 +2.7 1,013,379 - Pf6N2E2_864 0.82 -0.5 1,013,427 + Pf6N2E2_864 0.88 -0.9 1,013,427 + Pf6N2E2_864 0.88 -0.1 1,013,427 + Pf6N2E2_864 0.88 +0.6 1,013,428 - Pf6N2E2_864 0.88 -0.1 1,013,537 - +1.5 1,013,537 - +0.5
Or see this region's nucleotide sequence