Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_758 and Pf6N2E2_759 are separated by 8 nucleotides Pf6N2E2_759 and Pf6N2E2_760 are separated by 163 nucleotides Pf6N2E2_760 and Pf6N2E2_761 are separated by 95 nucleotides
Pf6N2E2_758 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 867,937 to 869,457
_758
Pf6N2E2_759 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 869,466 to 870,275
_759
Pf6N2E2_760 - Na+/H+ antiporter NhaB, at 870,439 to 871,941
_760
Pf6N2E2_761 - Hemolysin-type calcium-binding region, at 872,037 to 873,326
_761
Position (kb)
870
871
872 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 869.508 kb on - strand at 869.718 kb on + strand, within Pf6N2E2_759 at 869.719 kb on - strand, within Pf6N2E2_759 at 869.719 kb on - strand, within Pf6N2E2_759 at 869.788 kb on + strand, within Pf6N2E2_759 at 869.788 kb on + strand, within Pf6N2E2_759 at 869.789 kb on - strand, within Pf6N2E2_759 at 869.789 kb on - strand, within Pf6N2E2_759 at 869.895 kb on + strand, within Pf6N2E2_759 at 869.895 kb on + strand, within Pf6N2E2_759 at 869.895 kb on + strand, within Pf6N2E2_759 at 869.895 kb on + strand, within Pf6N2E2_759 at 869.895 kb on + strand, within Pf6N2E2_759 at 869.896 kb on - strand, within Pf6N2E2_759 at 869.896 kb on - strand, within Pf6N2E2_759 at 869.896 kb on - strand, within Pf6N2E2_759 at 870.018 kb on + strand, within Pf6N2E2_759 at 870.258 kb on + strand at 870.258 kb on + strand at 870.335 kb on - strand at 870.335 kb on - strand at 870.335 kb on - strand at 870.434 kb on + strand at 870.435 kb on - strand at 870.435 kb on - strand at 870.435 kb on - strand at 870.435 kb on - strand at 870.435 kb on - strand at 870.440 kb on + strand at 870.440 kb on + strand at 870.440 kb on + strand at 870.441 kb on - strand at 870.449 kb on + strand at 870.450 kb on - strand at 870.450 kb on - strand at 870.450 kb on - strand at 870.462 kb on - strand at 870.492 kb on - strand at 870.522 kb on - strand at 870.693 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.699 kb on - strand, within Pf6N2E2_760 at 871.023 kb on + strand, within Pf6N2E2_760 at 871.024 kb on - strand, within Pf6N2E2_760 at 871.024 kb on - strand, within Pf6N2E2_760 at 871.085 kb on + strand, within Pf6N2E2_760 at 871.085 kb on + strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.336 kb on + strand, within Pf6N2E2_760 at 871.337 kb on - strand, within Pf6N2E2_760 at 871.446 kb on - strand, within Pf6N2E2_760 at 871.454 kb on + strand, within Pf6N2E2_760 at 871.454 kb on + strand, within Pf6N2E2_760 at 871.454 kb on + strand, within Pf6N2E2_760 at 871.454 kb on + strand, within Pf6N2E2_760 at 871.455 kb on - strand, within Pf6N2E2_760 at 871.455 kb on - strand, within Pf6N2E2_760 at 871.455 kb on - strand, within Pf6N2E2_760 at 871.619 kb on + strand, within Pf6N2E2_760 at 871.619 kb on + strand, within Pf6N2E2_760 at 871.619 kb on + strand, within Pf6N2E2_760 at 871.620 kb on - strand, within Pf6N2E2_760 at 871.620 kb on - strand, within Pf6N2E2_760 at 871.682 kb on + strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.761 kb on + strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.880 kb on + strand at 871.880 kb on + strand at 871.881 kb on - strand at 871.881 kb on - strand at 871.881 kb on - strand at 871.881 kb on - strand at 871.908 kb on + strand at 871.908 kb on + strand at 871.955 kb on + strand at 871.993 kb on + strand at 872.008 kb on + strand at 872.008 kb on + strand at 872.012 kb on + strand at 872.012 kb on + strand at 872.012 kb on + strand at 872.012 kb on + strand at 872.120 kb on + strand at 872.214 kb on + strand, within Pf6N2E2_761 at 872.215 kb on - strand, within Pf6N2E2_761 at 872.406 kb on - strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.607 kb on - strand, within Pf6N2E2_761 at 872.607 kb on - strand, within Pf6N2E2_761 at 872.607 kb on - strand, within Pf6N2E2_761 at 872.646 kb on - strand, within Pf6N2E2_761 at 872.646 kb on - strand, within Pf6N2E2_761 at 872.756 kb on - strand, within Pf6N2E2_761 at 872.796 kb on + strand, within Pf6N2E2_761
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 869,508 - +0.1 869,718 + Pf6N2E2_759 0.31 -0.1 869,719 - Pf6N2E2_759 0.31 -0.1 869,719 - Pf6N2E2_759 0.31 +1.3 869,788 + Pf6N2E2_759 0.40 -0.4 869,788 + Pf6N2E2_759 0.40 +1.3 869,789 - Pf6N2E2_759 0.40 -0.0 869,789 - Pf6N2E2_759 0.40 -0.2 869,895 + Pf6N2E2_759 0.53 -0.1 869,895 + Pf6N2E2_759 0.53 -0.3 869,895 + Pf6N2E2_759 0.53 +0.8 869,895 + Pf6N2E2_759 0.53 -0.2 869,895 + Pf6N2E2_759 0.53 +0.6 869,896 - Pf6N2E2_759 0.53 +0.2 869,896 - Pf6N2E2_759 0.53 -0.6 869,896 - Pf6N2E2_759 0.53 +0.1 870,018 + Pf6N2E2_759 0.68 +0.2 870,258 + -0.2 870,258 + -1.2 870,335 - -0.0 870,335 - +0.9 870,335 - -1.2 870,434 + -2.0 870,435 - +0.2 870,435 - +0.2 870,435 - +0.1 870,435 - -0.6 870,435 - +0.6 870,440 + +0.7 870,440 + -0.9 870,440 + +0.4 870,441 - -2.1 870,449 + -0.0 870,450 - -2.6 870,450 - +0.2 870,450 - +0.6 870,462 - -1.1 870,492 - -1.5 870,522 - -2.2 870,693 + Pf6N2E2_760 0.17 -2.4 870,698 + Pf6N2E2_760 0.17 -0.7 870,698 + Pf6N2E2_760 0.17 -1.5 870,698 + Pf6N2E2_760 0.17 -1.0 870,698 + Pf6N2E2_760 0.17 -1.1 870,698 + Pf6N2E2_760 0.17 -2.7 870,698 + Pf6N2E2_760 0.17 -1.1 870,698 + Pf6N2E2_760 0.17 -0.1 870,698 + Pf6N2E2_760 0.17 -2.1 870,698 + Pf6N2E2_760 0.17 -1.9 870,699 - Pf6N2E2_760 0.17 -1.9 871,023 + Pf6N2E2_760 0.39 +1.5 871,024 - Pf6N2E2_760 0.39 -1.4 871,024 - Pf6N2E2_760 0.39 -1.0 871,085 + Pf6N2E2_760 0.43 -1.8 871,085 + Pf6N2E2_760 0.43 -0.7 871,106 - Pf6N2E2_760 0.44 -1.2 871,106 - Pf6N2E2_760 0.44 -1.9 871,106 - Pf6N2E2_760 0.44 +1.5 871,106 - Pf6N2E2_760 0.44 -1.0 871,106 - Pf6N2E2_760 0.44 +1.5 871,106 - Pf6N2E2_760 0.44 -1.3 871,106 - Pf6N2E2_760 0.44 -1.8 871,106 - Pf6N2E2_760 0.44 -2.4 871,106 - Pf6N2E2_760 0.44 -3.1 871,336 + Pf6N2E2_760 0.60 -3.3 871,337 - Pf6N2E2_760 0.60 -1.5 871,446 - Pf6N2E2_760 0.67 -1.3 871,454 + Pf6N2E2_760 0.68 +0.2 871,454 + Pf6N2E2_760 0.68 +0.1 871,454 + Pf6N2E2_760 0.68 -2.8 871,454 + Pf6N2E2_760 0.68 -2.5 871,455 - Pf6N2E2_760 0.68 -0.9 871,455 - Pf6N2E2_760 0.68 -1.7 871,455 - Pf6N2E2_760 0.68 -2.6 871,619 + Pf6N2E2_760 0.79 -2.8 871,619 + Pf6N2E2_760 0.79 -0.4 871,619 + Pf6N2E2_760 0.79 -1.8 871,620 - Pf6N2E2_760 0.79 -1.3 871,620 - Pf6N2E2_760 0.79 +0.9 871,682 + Pf6N2E2_760 0.83 -1.9 871,683 - Pf6N2E2_760 0.83 -2.1 871,683 - Pf6N2E2_760 0.83 -1.7 871,683 - Pf6N2E2_760 0.83 -2.4 871,683 - Pf6N2E2_760 0.83 -0.8 871,683 - Pf6N2E2_760 0.83 -1.2 871,683 - Pf6N2E2_760 0.83 -2.7 871,761 + Pf6N2E2_760 0.88 -2.1 871,762 - Pf6N2E2_760 0.88 -1.9 871,762 - Pf6N2E2_760 0.88 -2.7 871,762 - Pf6N2E2_760 0.88 -2.2 871,762 - Pf6N2E2_760 0.88 -1.4 871,762 - Pf6N2E2_760 0.88 -1.8 871,762 - Pf6N2E2_760 0.88 -3.0 871,880 + -3.1 871,880 + -1.4 871,881 - -0.8 871,881 - +0.5 871,881 - -2.2 871,881 - -1.8 871,908 + -1.2 871,908 + +1.5 871,955 + +4.3 871,993 + -0.1 872,008 + -0.5 872,008 + -1.2 872,012 + -1.5 872,012 + +0.1 872,012 + -1.5 872,012 + -1.5 872,120 + -0.7 872,214 + Pf6N2E2_761 0.14 -0.2 872,215 - Pf6N2E2_761 0.14 +0.3 872,406 - Pf6N2E2_761 0.29 +2.0 872,606 + Pf6N2E2_761 0.44 -0.7 872,606 + Pf6N2E2_761 0.44 -0.1 872,606 + Pf6N2E2_761 0.44 -0.1 872,606 + Pf6N2E2_761 0.44 +0.7 872,606 + Pf6N2E2_761 0.44 -2.6 872,606 + Pf6N2E2_761 0.44 -0.3 872,606 + Pf6N2E2_761 0.44 +2.5 872,607 - Pf6N2E2_761 0.44 +0.6 872,607 - Pf6N2E2_761 0.44 +0.5 872,607 - Pf6N2E2_761 0.44 -0.0 872,646 - Pf6N2E2_761 0.47 +1.5 872,646 - Pf6N2E2_761 0.47 +1.1 872,756 - Pf6N2E2_761 0.56 +0.5 872,796 + Pf6N2E2_761 0.59 +1.5
Or see this region's nucleotide sequence