Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_731

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_730 and Pf6N2E2_731 are separated by 2 nucleotidesPf6N2E2_731 and Pf6N2E2_732 overlap by 8 nucleotidesPf6N2E2_732 and Pf6N2E2_733 are separated by 10 nucleotides Pf6N2E2_730 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 838,011 to 839,549 _730 Pf6N2E2_731 - Respiratory nitrate reductase delta chain (EC 1.7.99.4), at 839,552 to 840,307 _731 Pf6N2E2_732 - Respiratory nitrate reductase gamma chain (EC 1.7.99.4), at 840,300 to 840,980 _732 Pf6N2E2_733 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 840,991 to 841,959 _733 Position (kb) 839 840 841Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 838.611 kb on + strand, within Pf6N2E2_730at 838.612 kb on - strand, within Pf6N2E2_730at 838.612 kb on - strand, within Pf6N2E2_730at 838.612 kb on - strand, within Pf6N2E2_730at 838.692 kb on + strand, within Pf6N2E2_730at 838.692 kb on + strand, within Pf6N2E2_730at 838.693 kb on - strand, within Pf6N2E2_730at 838.693 kb on - strand, within Pf6N2E2_730at 838.693 kb on - strand, within Pf6N2E2_730at 838.884 kb on + strand, within Pf6N2E2_730at 838.885 kb on - strand, within Pf6N2E2_730at 839.071 kb on - strand, within Pf6N2E2_730at 839.172 kb on + strand, within Pf6N2E2_730at 839.251 kb on - strand, within Pf6N2E2_730at 839.337 kb on + strand, within Pf6N2E2_730at 839.338 kb on - strand, within Pf6N2E2_730at 839.338 kb on - strand, within Pf6N2E2_730at 839.338 kb on - strand, within Pf6N2E2_730at 839.361 kb on + strand, within Pf6N2E2_730at 839.361 kb on + strand, within Pf6N2E2_730at 839.361 kb on + strand, within Pf6N2E2_730at 839.362 kb on - strand, within Pf6N2E2_730at 839.362 kb on - strand, within Pf6N2E2_730at 839.362 kb on - strand, within Pf6N2E2_730at 839.380 kb on + strand, within Pf6N2E2_730at 839.880 kb on + strand, within Pf6N2E2_731at 839.880 kb on + strand, within Pf6N2E2_731at 839.881 kb on - strand, within Pf6N2E2_731at 839.881 kb on - strand, within Pf6N2E2_731at 839.881 kb on - strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 839.888 kb on + strand, within Pf6N2E2_731at 840.298 kb on + strandat 840.298 kb on + strandat 840.305 kb on + strandat 840.305 kb on + strandat 840.306 kb on - strandat 840.306 kb on - strandat 840.396 kb on + strand, within Pf6N2E2_732at 840.397 kb on - strand, within Pf6N2E2_732at 840.397 kb on - strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.528 kb on + strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.529 kb on - strand, within Pf6N2E2_732at 840.740 kb on + strand, within Pf6N2E2_732at 840.852 kb on + strand, within Pf6N2E2_732at 840.852 kb on + strand, within Pf6N2E2_732at 840.853 kb on - strand, within Pf6N2E2_732at 840.940 kb on - strandat 840.940 kb on - strandat 841.246 kb on + strand, within Pf6N2E2_733at 841.247 kb on - strand, within Pf6N2E2_733

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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838,611 + Pf6N2E2_730 0.39 -1.4
838,612 - Pf6N2E2_730 0.39 -4.4
838,612 - Pf6N2E2_730 0.39 +0.4
838,612 - Pf6N2E2_730 0.39 -4.1
838,692 + Pf6N2E2_730 0.44 -3.4
838,692 + Pf6N2E2_730 0.44 -3.5
838,693 - Pf6N2E2_730 0.44 -1.7
838,693 - Pf6N2E2_730 0.44 -3.8
838,693 - Pf6N2E2_730 0.44 +0.4
838,884 + Pf6N2E2_730 0.57 -1.2
838,885 - Pf6N2E2_730 0.57 -3.4
839,071 - Pf6N2E2_730 0.69 -2.5
839,172 + Pf6N2E2_730 0.75 -3.7
839,251 - Pf6N2E2_730 0.81 -1.7
839,337 + Pf6N2E2_730 0.86 +1.4
839,338 - Pf6N2E2_730 0.86 -3.9
839,338 - Pf6N2E2_730 0.86 -1.6
839,338 - Pf6N2E2_730 0.86 -1.6
839,361 + Pf6N2E2_730 0.88 -2.0
839,361 + Pf6N2E2_730 0.88 +1.4
839,361 + Pf6N2E2_730 0.88 -2.7
839,362 - Pf6N2E2_730 0.88 -2.3
839,362 - Pf6N2E2_730 0.88 -3.8
839,362 - Pf6N2E2_730 0.88 -3.3
839,380 + Pf6N2E2_730 0.89 -3.1
839,880 + Pf6N2E2_731 0.43 -0.6
839,880 + Pf6N2E2_731 0.43 -2.8
839,881 - Pf6N2E2_731 0.44 -2.6
839,881 - Pf6N2E2_731 0.44 -0.1
839,881 - Pf6N2E2_731 0.44 -3.4
839,888 + Pf6N2E2_731 0.44 -1.6
839,888 + Pf6N2E2_731 0.44 -3.6
839,888 + Pf6N2E2_731 0.44 -1.0
839,888 + Pf6N2E2_731 0.44 -5.2
839,888 + Pf6N2E2_731 0.44 -2.3
839,888 + Pf6N2E2_731 0.44 -3.1
839,888 + Pf6N2E2_731 0.44 -4.1
840,298 + -2.3
840,298 + -0.6
840,305 + -0.3
840,305 + -1.1
840,306 - -1.7
840,306 - -2.5
840,396 + Pf6N2E2_732 0.14 -2.3
840,397 - Pf6N2E2_732 0.14 -1.4
840,397 - Pf6N2E2_732 0.14 -4.5
840,528 + Pf6N2E2_732 0.33 -3.6
840,528 + Pf6N2E2_732 0.33 -2.7
840,528 + Pf6N2E2_732 0.33 -3.0
840,528 + Pf6N2E2_732 0.33 -2.0
840,528 + Pf6N2E2_732 0.33 -3.2
840,528 + Pf6N2E2_732 0.33 -2.2
840,528 + Pf6N2E2_732 0.33 -1.4
840,528 + Pf6N2E2_732 0.33 -0.6
840,529 - Pf6N2E2_732 0.34 +0.4
840,529 - Pf6N2E2_732 0.34 +1.4
840,529 - Pf6N2E2_732 0.34 -1.1
840,529 - Pf6N2E2_732 0.34 -2.8
840,529 - Pf6N2E2_732 0.34 +0.9
840,529 - Pf6N2E2_732 0.34 -2.9
840,740 + Pf6N2E2_732 0.65 -3.0
840,852 + Pf6N2E2_732 0.81 -2.7
840,852 + Pf6N2E2_732 0.81 -3.6
840,853 - Pf6N2E2_732 0.81 -1.7
840,940 - -1.1
840,940 - -1.6
841,246 + Pf6N2E2_733 0.26 -1.8
841,247 - Pf6N2E2_733 0.26 -1.6

Or see this region's nucleotide sequence