Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_729

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_728 and Pf6N2E2_729 are separated by 64 nucleotidesPf6N2E2_729 and Pf6N2E2_730 are separated by 1 nucleotides Pf6N2E2_728 - Nitrate/nitrite transporter, at 832,774 to 834,171 _728 Pf6N2E2_729 - Respiratory nitrate reductase alpha chain (EC 1.7.99.4), at 834,236 to 838,009 _729 Pf6N2E2_730 - Respiratory nitrate reductase beta chain (EC 1.7.99.4), at 838,011 to 839,549 _730 Position (kb) 834 835 836 837 838 839Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 833.239 kb on + strand, within Pf6N2E2_728at 833.375 kb on + strand, within Pf6N2E2_728at 833.812 kb on + strand, within Pf6N2E2_728at 833.813 kb on - strand, within Pf6N2E2_728at 834.036 kb on + strandat 834.036 kb on + strandat 834.037 kb on - strandat 834.088 kb on + strandat 834.088 kb on + strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.139 kb on + strandat 834.139 kb on + strandat 834.170 kb on - strandat 834.180 kb on + strandat 834.246 kb on + strandat 834.247 kb on - strandat 834.275 kb on - strandat 834.275 kb on - strandat 834.341 kb on + strandat 834.359 kb on + strandat 834.359 kb on + strandat 834.423 kb on - strandat 834.489 kb on + strandat 834.490 kb on - strandat 834.540 kb on - strandat 834.540 kb on - strandat 834.540 kb on - strandat 834.540 kb on - strandat 834.545 kb on + strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.678 kb on - strand, within Pf6N2E2_729at 834.678 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.758 kb on + strand, within Pf6N2E2_729at 834.758 kb on + strand, within Pf6N2E2_729at 834.759 kb on - strand, within Pf6N2E2_729at 834.830 kb on + strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.965 kb on + strand, within Pf6N2E2_729at 834.978 kb on - strand, within Pf6N2E2_729at 834.978 kb on - strand, within Pf6N2E2_729at 834.982 kb on + strand, within Pf6N2E2_729at 835.049 kb on + strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.085 kb on + strand, within Pf6N2E2_729at 835.085 kb on + strand, within Pf6N2E2_729at 835.086 kb on - strand, within Pf6N2E2_729at 835.233 kb on - strand, within Pf6N2E2_729at 835.542 kb on - strand, within Pf6N2E2_729at 835.542 kb on - strand, within Pf6N2E2_729at 835.565 kb on + strand, within Pf6N2E2_729at 835.565 kb on + strand, within Pf6N2E2_729at 835.566 kb on - strand, within Pf6N2E2_729at 835.566 kb on - strand, within Pf6N2E2_729at 835.730 kb on + strand, within Pf6N2E2_729at 835.730 kb on + strand, within Pf6N2E2_729at 835.730 kb on + strand, within Pf6N2E2_729at 835.907 kb on + strand, within Pf6N2E2_729at 835.976 kb on + strand, within Pf6N2E2_729at 835.976 kb on + strand, within Pf6N2E2_729at 835.976 kb on + strand, within Pf6N2E2_729at 835.976 kb on + strand, within Pf6N2E2_729at 835.976 kb on + strand, within Pf6N2E2_729at 835.976 kb on + strand, within Pf6N2E2_729at 835.976 kb on + strand, within Pf6N2E2_729at 835.976 kb on + strand, within Pf6N2E2_729at 835.976 kb on + strand, within Pf6N2E2_729at 836.082 kb on - strand, within Pf6N2E2_729at 836.082 kb on - strand, within Pf6N2E2_729at 836.082 kb on - strand, within Pf6N2E2_729at 836.181 kb on - strand, within Pf6N2E2_729at 836.181 kb on - strand, within Pf6N2E2_729at 836.291 kb on + strand, within Pf6N2E2_729at 836.292 kb on - strand, within Pf6N2E2_729at 836.292 kb on - strand, within Pf6N2E2_729at 836.292 kb on - strand, within Pf6N2E2_729at 836.423 kb on + strand, within Pf6N2E2_729at 836.423 kb on + strand, within Pf6N2E2_729at 836.424 kb on - strand, within Pf6N2E2_729at 836.435 kb on + strand, within Pf6N2E2_729at 836.435 kb on + strand, within Pf6N2E2_729at 836.436 kb on - strand, within Pf6N2E2_729at 836.482 kb on + strand, within Pf6N2E2_729at 836.483 kb on - strand, within Pf6N2E2_729at 836.483 kb on - strand, within Pf6N2E2_729at 836.621 kb on + strand, within Pf6N2E2_729at 836.622 kb on - strand, within Pf6N2E2_729at 836.724 kb on - strand, within Pf6N2E2_729at 836.933 kb on + strand, within Pf6N2E2_729at 836.933 kb on + strand, within Pf6N2E2_729at 836.933 kb on + strand, within Pf6N2E2_729at 836.933 kb on + strand, within Pf6N2E2_729at 836.934 kb on - strand, within Pf6N2E2_729at 836.934 kb on - strand, within Pf6N2E2_729at 836.934 kb on - strand, within Pf6N2E2_729at 836.934 kb on - strand, within Pf6N2E2_729at 837.315 kb on - strand, within Pf6N2E2_729at 837.315 kb on - strand, within Pf6N2E2_729at 837.383 kb on + strand, within Pf6N2E2_729at 837.384 kb on - strand, within Pf6N2E2_729at 837.551 kb on + strand, within Pf6N2E2_729at 837.551 kb on + strand, within Pf6N2E2_729at 837.551 kb on + strand, within Pf6N2E2_729at 837.552 kb on - strand, within Pf6N2E2_729at 837.552 kb on - strand, within Pf6N2E2_729at 837.552 kb on - strand, within Pf6N2E2_729at 837.552 kb on - strand, within Pf6N2E2_729at 837.734 kb on + strandat 837.734 kb on + strandat 837.734 kb on + strandat 837.734 kb on + strandat 837.734 kb on + strandat 837.734 kb on + strandat 837.734 kb on + strandat 837.735 kb on - strandat 837.735 kb on - strandat 837.735 kb on - strandat 837.735 kb on - strandat 837.735 kb on - strandat 837.735 kb on - strandat 837.879 kb on - strandat 837.879 kb on - strandat 837.879 kb on - strandat 837.879 kb on - strandat 838.122 kb on + strandat 838.123 kb on - strandat 838.123 kb on - strandat 838.308 kb on + strand, within Pf6N2E2_730at 838.309 kb on - strand, within Pf6N2E2_730at 838.311 kb on + strand, within Pf6N2E2_730at 838.311 kb on + strand, within Pf6N2E2_730at 838.512 kb on + strand, within Pf6N2E2_730at 838.513 kb on - strand, within Pf6N2E2_730at 838.542 kb on + strand, within Pf6N2E2_730at 838.542 kb on + strand, within Pf6N2E2_730at 838.542 kb on + strand, within Pf6N2E2_730at 838.542 kb on + strand, within Pf6N2E2_730at 838.542 kb on + strand, within Pf6N2E2_730at 838.543 kb on - strand, within Pf6N2E2_730at 838.543 kb on - strand, within Pf6N2E2_730at 838.543 kb on - strand, within Pf6N2E2_730at 838.611 kb on + strand, within Pf6N2E2_730at 838.612 kb on - strand, within Pf6N2E2_730at 838.612 kb on - strand, within Pf6N2E2_730at 838.612 kb on - strand, within Pf6N2E2_730at 838.692 kb on + strand, within Pf6N2E2_730at 838.692 kb on + strand, within Pf6N2E2_730at 838.693 kb on - strand, within Pf6N2E2_730at 838.693 kb on - strand, within Pf6N2E2_730at 838.693 kb on - strand, within Pf6N2E2_730at 838.884 kb on + strand, within Pf6N2E2_730at 838.885 kb on - strand, within Pf6N2E2_730

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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833,239 + Pf6N2E2_728 0.33 +1.4
833,375 + Pf6N2E2_728 0.43 -3.7
833,812 + Pf6N2E2_728 0.74 +0.4
833,813 - Pf6N2E2_728 0.74 -3.6
834,036 + +1.4
834,036 + +1.4
834,037 - +0.4
834,088 + -2.8
834,088 + -1.7
834,089 - -3.4
834,089 - -1.1
834,089 - -2.3
834,089 - -4.4
834,089 - -2.3
834,089 - -0.7
834,139 + -1.8
834,139 + +1.9
834,170 - -0.6
834,180 + -1.6
834,246 + -1.9
834,247 - -0.0
834,275 - -2.1
834,275 - -2.3
834,341 + -0.9
834,359 + -2.5
834,359 + -1.8
834,423 - +1.4
834,489 + -2.6
834,490 - -3.8
834,540 - -3.1
834,540 - -0.6
834,540 - -1.0
834,540 - -2.6
834,545 + +0.4
834,546 - -3.0
834,546 - -1.6
834,546 - +0.4
834,546 - -2.8
834,546 - -2.3
834,678 - Pf6N2E2_729 0.12 -2.6
834,678 - Pf6N2E2_729 0.12 +0.4
834,680 - Pf6N2E2_729 0.12 -3.3
834,680 - Pf6N2E2_729 0.12 -3.3
834,680 - Pf6N2E2_729 0.12 -3.5
834,680 - Pf6N2E2_729 0.12 -0.1
834,680 - Pf6N2E2_729 0.12 -4.0
834,680 - Pf6N2E2_729 0.12 -0.8
834,758 + Pf6N2E2_729 0.14 -2.3
834,758 + Pf6N2E2_729 0.14 -4.0
834,759 - Pf6N2E2_729 0.14 -2.3
834,830 + Pf6N2E2_729 0.16 -1.8
834,885 - Pf6N2E2_729 0.17 -3.9
834,885 - Pf6N2E2_729 0.17 -2.3
834,885 - Pf6N2E2_729 0.17 -2.4
834,885 - Pf6N2E2_729 0.17 -2.0
834,885 - Pf6N2E2_729 0.17 -0.4
834,965 + Pf6N2E2_729 0.19 -0.1
834,978 - Pf6N2E2_729 0.20 -4.0
834,978 - Pf6N2E2_729 0.20 +0.1
834,982 + Pf6N2E2_729 0.20 -2.2
835,049 + Pf6N2E2_729 0.22 -2.5
835,050 - Pf6N2E2_729 0.22 -3.3
835,050 - Pf6N2E2_729 0.22 -2.8
835,050 - Pf6N2E2_729 0.22 -1.5
835,050 - Pf6N2E2_729 0.22 -2.3
835,085 + Pf6N2E2_729 0.22 -2.4
835,085 + Pf6N2E2_729 0.22 -2.7
835,086 - Pf6N2E2_729 0.23 -4.0
835,233 - Pf6N2E2_729 0.26 -1.3
835,542 - Pf6N2E2_729 0.35 -2.8
835,542 - Pf6N2E2_729 0.35 -3.0
835,565 + Pf6N2E2_729 0.35 -0.3
835,565 + Pf6N2E2_729 0.35 -2.0
835,566 - Pf6N2E2_729 0.35 -3.4
835,566 - Pf6N2E2_729 0.35 -2.4
835,730 + Pf6N2E2_729 0.40 -0.3
835,730 + Pf6N2E2_729 0.40 -2.7
835,730 + Pf6N2E2_729 0.40 -3.1
835,907 + Pf6N2E2_729 0.44 -3.0
835,976 + Pf6N2E2_729 0.46 -3.1
835,976 + Pf6N2E2_729 0.46 -3.4
835,976 + Pf6N2E2_729 0.46 -1.7
835,976 + Pf6N2E2_729 0.46 -1.8
835,976 + Pf6N2E2_729 0.46 -2.8
835,976 + Pf6N2E2_729 0.46 -1.4
835,976 + Pf6N2E2_729 0.46 -1.7
835,976 + Pf6N2E2_729 0.46 -2.8
835,976 + Pf6N2E2_729 0.46 -1.8
836,082 - Pf6N2E2_729 0.49 -2.6
836,082 - Pf6N2E2_729 0.49 -4.4
836,082 - Pf6N2E2_729 0.49 -1.9
836,181 - Pf6N2E2_729 0.52 -2.6
836,181 - Pf6N2E2_729 0.52 -2.8
836,291 + Pf6N2E2_729 0.54 +0.4
836,292 - Pf6N2E2_729 0.54 -1.4
836,292 - Pf6N2E2_729 0.54 -2.2
836,292 - Pf6N2E2_729 0.54 -4.1
836,423 + Pf6N2E2_729 0.58 -1.7
836,423 + Pf6N2E2_729 0.58 +0.4
836,424 - Pf6N2E2_729 0.58 -0.9
836,435 + Pf6N2E2_729 0.58 -3.4
836,435 + Pf6N2E2_729 0.58 +0.4
836,436 - Pf6N2E2_729 0.58 -2.3
836,482 + Pf6N2E2_729 0.60 -1.6
836,483 - Pf6N2E2_729 0.60 -2.9
836,483 - Pf6N2E2_729 0.60 -3.4
836,621 + Pf6N2E2_729 0.63 -2.5
836,622 - Pf6N2E2_729 0.63 -1.6
836,724 - Pf6N2E2_729 0.66 -2.7
836,933 + Pf6N2E2_729 0.71 -2.1
836,933 + Pf6N2E2_729 0.71 -3.2
836,933 + Pf6N2E2_729 0.71 -2.1
836,933 + Pf6N2E2_729 0.71 -1.1
836,934 - Pf6N2E2_729 0.71 -1.8
836,934 - Pf6N2E2_729 0.71 -2.4
836,934 - Pf6N2E2_729 0.71 -3.3
836,934 - Pf6N2E2_729 0.71 -1.1
837,315 - Pf6N2E2_729 0.82 -2.1
837,315 - Pf6N2E2_729 0.82 -3.8
837,383 + Pf6N2E2_729 0.83 -4.0
837,384 - Pf6N2E2_729 0.83 -4.0
837,551 + Pf6N2E2_729 0.88 -4.2
837,551 + Pf6N2E2_729 0.88 -3.6
837,551 + Pf6N2E2_729 0.88 -1.4
837,552 - Pf6N2E2_729 0.88 -4.0
837,552 - Pf6N2E2_729 0.88 -1.8
837,552 - Pf6N2E2_729 0.88 -1.6
837,552 - Pf6N2E2_729 0.88 -4.7
837,734 + -2.1
837,734 + +1.4
837,734 + +1.4
837,734 + -2.6
837,734 + -3.6
837,734 + -0.8
837,734 + -1.5
837,735 - -2.0
837,735 - -1.7
837,735 - +1.4
837,735 - -2.5
837,735 - -3.6
837,735 - -3.0
837,879 - -1.6
837,879 - -4.0
837,879 - -3.7
837,879 - -3.6
838,122 + -3.3
838,123 - +0.4
838,123 - -0.1
838,308 + Pf6N2E2_730 0.19 -1.1
838,309 - Pf6N2E2_730 0.19 -0.6
838,311 + Pf6N2E2_730 0.19 -3.5
838,311 + Pf6N2E2_730 0.19 -4.0
838,512 + Pf6N2E2_730 0.33 -1.4
838,513 - Pf6N2E2_730 0.33 -3.9
838,542 + Pf6N2E2_730 0.35 -2.6
838,542 + Pf6N2E2_730 0.35 -1.1
838,542 + Pf6N2E2_730 0.35 -2.0
838,542 + Pf6N2E2_730 0.35 -1.1
838,542 + Pf6N2E2_730 0.35 -2.1
838,543 - Pf6N2E2_730 0.35 -1.1
838,543 - Pf6N2E2_730 0.35 -2.0
838,543 - Pf6N2E2_730 0.35 +1.4
838,611 + Pf6N2E2_730 0.39 -1.4
838,612 - Pf6N2E2_730 0.39 -4.4
838,612 - Pf6N2E2_730 0.39 +0.4
838,612 - Pf6N2E2_730 0.39 -4.1
838,692 + Pf6N2E2_730 0.44 -3.4
838,692 + Pf6N2E2_730 0.44 -3.5
838,693 - Pf6N2E2_730 0.44 -1.7
838,693 - Pf6N2E2_730 0.44 -3.8
838,693 - Pf6N2E2_730 0.44 +0.4
838,884 + Pf6N2E2_730 0.57 -1.2
838,885 - Pf6N2E2_730 0.57 -3.4

Or see this region's nucleotide sequence