Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_728

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_727 and Pf6N2E2_728 overlap by 23 nucleotidesPf6N2E2_728 and Pf6N2E2_729 are separated by 64 nucleotides Pf6N2E2_727 - Nitrate/nitrite transporter, at 831,495 to 832,796 _727 Pf6N2E2_728 - Nitrate/nitrite transporter, at 832,774 to 834,171 _728 Pf6N2E2_729 - Respiratory nitrate reductase alpha chain (EC 1.7.99.4), at 834,236 to 838,009 _729 Position (kb) 832 833 834 835Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 831.852 kb on + strand, within Pf6N2E2_727at 831.852 kb on + strand, within Pf6N2E2_727at 831.852 kb on + strand, within Pf6N2E2_727at 832.134 kb on + strand, within Pf6N2E2_727at 832.135 kb on - strand, within Pf6N2E2_727at 832.135 kb on - strand, within Pf6N2E2_727at 832.137 kb on + strand, within Pf6N2E2_727at 832.159 kb on - strand, within Pf6N2E2_727at 832.159 kb on - strand, within Pf6N2E2_727at 832.188 kb on + strand, within Pf6N2E2_727at 832.188 kb on + strand, within Pf6N2E2_727at 832.189 kb on - strand, within Pf6N2E2_727at 832.189 kb on - strand, within Pf6N2E2_727at 832.189 kb on - strand, within Pf6N2E2_727at 832.189 kb on - strand, within Pf6N2E2_727at 832.191 kb on + strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.503 kb on + strand, within Pf6N2E2_727at 832.503 kb on + strand, within Pf6N2E2_727at 832.504 kb on - strand, within Pf6N2E2_727at 832.504 kb on - strand, within Pf6N2E2_727at 832.650 kb on + strand, within Pf6N2E2_727at 832.650 kb on + strand, within Pf6N2E2_727at 832.651 kb on - strand, within Pf6N2E2_727at 832.651 kb on - strand, within Pf6N2E2_727at 832.651 kb on - strand, within Pf6N2E2_727at 832.683 kb on + strandat 832.683 kb on + strandat 832.683 kb on + strandat 832.683 kb on + strandat 832.683 kb on + strandat 832.684 kb on - strandat 832.684 kb on - strandat 832.684 kb on - strandat 833.042 kb on - strand, within Pf6N2E2_728at 833.239 kb on + strand, within Pf6N2E2_728at 833.375 kb on + strand, within Pf6N2E2_728at 833.812 kb on + strand, within Pf6N2E2_728at 833.813 kb on - strand, within Pf6N2E2_728at 834.036 kb on + strandat 834.036 kb on + strandat 834.037 kb on - strandat 834.088 kb on + strandat 834.088 kb on + strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.139 kb on + strandat 834.139 kb on + strandat 834.170 kb on - strandat 834.180 kb on + strandat 834.246 kb on + strandat 834.247 kb on - strandat 834.275 kb on - strandat 834.275 kb on - strandat 834.341 kb on + strandat 834.359 kb on + strandat 834.359 kb on + strandat 834.423 kb on - strandat 834.489 kb on + strandat 834.490 kb on - strandat 834.540 kb on - strandat 834.540 kb on - strandat 834.540 kb on - strandat 834.540 kb on - strandat 834.545 kb on + strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.678 kb on - strand, within Pf6N2E2_729at 834.678 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.758 kb on + strand, within Pf6N2E2_729at 834.758 kb on + strand, within Pf6N2E2_729at 834.759 kb on - strand, within Pf6N2E2_729at 834.830 kb on + strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.965 kb on + strand, within Pf6N2E2_729at 834.978 kb on - strand, within Pf6N2E2_729at 834.978 kb on - strand, within Pf6N2E2_729at 834.982 kb on + strand, within Pf6N2E2_729at 835.049 kb on + strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.085 kb on + strand, within Pf6N2E2_729at 835.085 kb on + strand, within Pf6N2E2_729at 835.086 kb on - strand, within Pf6N2E2_729

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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831,852 + Pf6N2E2_727 0.27 -2.4
831,852 + Pf6N2E2_727 0.27 -2.0
831,852 + Pf6N2E2_727 0.27 -0.3
832,134 + Pf6N2E2_727 0.49 +0.4
832,135 - Pf6N2E2_727 0.49 -1.6
832,135 - Pf6N2E2_727 0.49 -1.3
832,137 + Pf6N2E2_727 0.49 -0.4
832,159 - Pf6N2E2_727 0.51 -3.1
832,159 - Pf6N2E2_727 0.51 -2.4
832,188 + Pf6N2E2_727 0.53 -0.9
832,188 + Pf6N2E2_727 0.53 -0.8
832,189 - Pf6N2E2_727 0.53 -3.2
832,189 - Pf6N2E2_727 0.53 -2.1
832,189 - Pf6N2E2_727 0.53 -1.1
832,189 - Pf6N2E2_727 0.53 -4.0
832,191 + Pf6N2E2_727 0.53 +0.2
832,192 - Pf6N2E2_727 0.54 -1.7
832,192 - Pf6N2E2_727 0.54 -3.7
832,192 - Pf6N2E2_727 0.54 +0.4
832,192 - Pf6N2E2_727 0.54 +1.4
832,192 - Pf6N2E2_727 0.54 -2.7
832,192 - Pf6N2E2_727 0.54 -1.7
832,503 + Pf6N2E2_727 0.77 +2.4
832,503 + Pf6N2E2_727 0.77 +0.9
832,504 - Pf6N2E2_727 0.77 -1.5
832,504 - Pf6N2E2_727 0.77 -0.1
832,650 + Pf6N2E2_727 0.89 +1.4
832,650 + Pf6N2E2_727 0.89 +1.3
832,651 - Pf6N2E2_727 0.89 -0.2
832,651 - Pf6N2E2_727 0.89 +0.4
832,651 - Pf6N2E2_727 0.89 -1.6
832,683 + +2.4
832,683 + +1.7
832,683 + +1.6
832,683 + +2.1
832,683 + +0.1
832,684 - -1.1
832,684 - +1.4
832,684 - -2.0
833,042 - Pf6N2E2_728 0.19 -1.5
833,239 + Pf6N2E2_728 0.33 +1.4
833,375 + Pf6N2E2_728 0.43 -3.7
833,812 + Pf6N2E2_728 0.74 +0.4
833,813 - Pf6N2E2_728 0.74 -3.6
834,036 + +1.4
834,036 + +1.4
834,037 - +0.4
834,088 + -2.8
834,088 + -1.7
834,089 - -3.4
834,089 - -1.1
834,089 - -2.3
834,089 - -4.4
834,089 - -2.3
834,089 - -0.7
834,139 + -1.8
834,139 + +1.9
834,170 - -0.6
834,180 + -1.6
834,246 + -1.9
834,247 - -0.0
834,275 - -2.1
834,275 - -2.3
834,341 + -0.9
834,359 + -2.5
834,359 + -1.8
834,423 - +1.4
834,489 + -2.6
834,490 - -3.8
834,540 - -3.1
834,540 - -0.6
834,540 - -1.0
834,540 - -2.6
834,545 + +0.4
834,546 - -3.0
834,546 - -1.6
834,546 - +0.4
834,546 - -2.8
834,546 - -2.3
834,678 - Pf6N2E2_729 0.12 -2.6
834,678 - Pf6N2E2_729 0.12 +0.4
834,680 - Pf6N2E2_729 0.12 -3.3
834,680 - Pf6N2E2_729 0.12 -3.3
834,680 - Pf6N2E2_729 0.12 -3.5
834,680 - Pf6N2E2_729 0.12 -0.1
834,680 - Pf6N2E2_729 0.12 -4.0
834,680 - Pf6N2E2_729 0.12 -0.8
834,758 + Pf6N2E2_729 0.14 -2.3
834,758 + Pf6N2E2_729 0.14 -4.0
834,759 - Pf6N2E2_729 0.14 -2.3
834,830 + Pf6N2E2_729 0.16 -1.8
834,885 - Pf6N2E2_729 0.17 -3.9
834,885 - Pf6N2E2_729 0.17 -2.3
834,885 - Pf6N2E2_729 0.17 -2.4
834,885 - Pf6N2E2_729 0.17 -2.0
834,885 - Pf6N2E2_729 0.17 -0.4
834,965 + Pf6N2E2_729 0.19 -0.1
834,978 - Pf6N2E2_729 0.20 -4.0
834,978 - Pf6N2E2_729 0.20 +0.1
834,982 + Pf6N2E2_729 0.20 -2.2
835,049 + Pf6N2E2_729 0.22 -2.5
835,050 - Pf6N2E2_729 0.22 -3.3
835,050 - Pf6N2E2_729 0.22 -2.8
835,050 - Pf6N2E2_729 0.22 -1.5
835,050 - Pf6N2E2_729 0.22 -2.3
835,085 + Pf6N2E2_729 0.22 -2.4
835,085 + Pf6N2E2_729 0.22 -2.7
835,086 - Pf6N2E2_729 0.23 -4.0

Or see this region's nucleotide sequence