Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_724 and Pf6N2E2_725 are separated by 229 nucleotides Pf6N2E2_725 and Pf6N2E2_726 overlap by 4 nucleotides
Pf6N2E2_724 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 827,836 to 828,513
_724
Pf6N2E2_725 - Nitrate/nitrite response regulator protein, at 828,743 to 829,390
_725
Pf6N2E2_726 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 829,387 to 831,186
_726
Position (kb) 828
829
830 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 828.193 kb on + strand, within Pf6N2E2_724 at 828.248 kb on - strand, within Pf6N2E2_724 at 828.488 kb on - strand at 828.539 kb on + strand at 828.540 kb on - strand at 828.563 kb on - strand at 828.563 kb on - strand at 828.711 kb on + strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.712 kb on - strand at 828.731 kb on + strand at 828.732 kb on - strand at 828.732 kb on - strand at 829.035 kb on + strand, within Pf6N2E2_725 at 829.035 kb on + strand, within Pf6N2E2_725 at 829.036 kb on - strand, within Pf6N2E2_725 at 829.155 kb on + strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.156 kb on - strand, within Pf6N2E2_725 at 829.361 kb on + strand at 829.362 kb on - strand at 829.856 kb on + strand, within Pf6N2E2_726 at 829.965 kb on - strand, within Pf6N2E2_726 at 830.076 kb on + strand, within Pf6N2E2_726 at 830.076 kb on + strand, within Pf6N2E2_726 at 830.220 kb on + strand, within Pf6N2E2_726
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 828,193 + Pf6N2E2_724 0.53 +0.4 828,248 - Pf6N2E2_724 0.61 +0.6 828,488 - +0.2 828,539 + +0.1 828,540 - -0.2 828,563 - -1.4 828,563 - -0.5 828,711 + -0.6 828,712 - -0.3 828,712 - -0.2 828,712 - +1.4 828,712 - +1.2 828,712 - -1.0 828,731 + -2.8 828,732 - +1.1 828,732 - -0.6 829,035 + Pf6N2E2_725 0.45 -2.4 829,035 + Pf6N2E2_725 0.45 -3.4 829,036 - Pf6N2E2_725 0.45 -3.6 829,155 + Pf6N2E2_725 0.64 -4.1 829,156 - Pf6N2E2_725 0.64 -3.2 829,156 - Pf6N2E2_725 0.64 -2.0 829,156 - Pf6N2E2_725 0.64 -2.2 829,156 - Pf6N2E2_725 0.64 +1.4 829,361 + -3.9 829,362 - -2.9 829,856 + Pf6N2E2_726 0.26 -2.6 829,965 - Pf6N2E2_726 0.32 -1.8 830,076 + Pf6N2E2_726 0.38 -4.2 830,076 + Pf6N2E2_726 0.38 -3.3 830,220 + Pf6N2E2_726 0.46 -1.6
Or see this region's nucleotide sequence