Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_718

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_717 and Pf6N2E2_718 are separated by 155 nucleotidesPf6N2E2_718 and Pf6N2E2_719 are separated by 88 nucleotidesPf6N2E2_719 and Pf6N2E2_720 overlap by 4 nucleotides Pf6N2E2_717 - Functional role page for Anaerobic nitric oxide reductase transcription regulator NorR, at 820,718 to 822,250 _717 Pf6N2E2_718 - Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5), at 822,406 to 823,053 _718 Pf6N2E2_719 - Molybdenum cofactor biosynthesis protein MoaB, at 823,142 to 823,696 _719 Pf6N2E2_720 - Molybdopterin biosynthesis protein MoeA, at 823,693 to 824,928 _720 Position (kb) 822 823 824Strain fitness (log2 ratio) -3 -2 -1 0 1at 821.554 kb on + strand, within Pf6N2E2_717at 821.744 kb on + strand, within Pf6N2E2_717at 821.745 kb on - strand, within Pf6N2E2_717at 821.745 kb on - strand, within Pf6N2E2_717at 821.745 kb on - strand, within Pf6N2E2_717at 822.220 kb on + strandat 822.220 kb on + strandat 822.220 kb on + strandat 822.221 kb on - strandat 822.650 kb on - strand, within Pf6N2E2_718at 822.732 kb on - strand, within Pf6N2E2_718at 822.732 kb on - strand, within Pf6N2E2_718at 822.732 kb on - strand, within Pf6N2E2_718at 822.782 kb on - strand, within Pf6N2E2_718at 823.067 kb on + strandat 823.067 kb on + strandat 823.067 kb on + strandat 823.068 kb on - strandat 823.068 kb on - strandat 823.084 kb on - strandat 823.193 kb on - strandat 823.303 kb on + strand, within Pf6N2E2_719at 823.308 kb on - strand, within Pf6N2E2_719at 823.886 kb on - strand, within Pf6N2E2_720

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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821,554 + Pf6N2E2_717 0.55 -2.8
821,744 + Pf6N2E2_717 0.67 +0.4
821,745 - Pf6N2E2_717 0.67 -2.2
821,745 - Pf6N2E2_717 0.67 -2.2
821,745 - Pf6N2E2_717 0.67 -1.0
822,220 + -1.4
822,220 + -2.7
822,220 + -1.7
822,221 - -3.0
822,650 - Pf6N2E2_718 0.38 -2.3
822,732 - Pf6N2E2_718 0.50 -3.4
822,732 - Pf6N2E2_718 0.50 -3.1
822,732 - Pf6N2E2_718 0.50 -0.7
822,782 - Pf6N2E2_718 0.58 -0.2
823,067 + +0.9
823,067 + +1.4
823,067 + -1.2
823,068 - +1.4
823,068 - +0.4
823,084 - +0.9
823,193 - -0.3
823,303 + Pf6N2E2_719 0.29 +1.2
823,308 - Pf6N2E2_719 0.30 -0.7
823,886 - Pf6N2E2_720 0.16 -0.6

Or see this region's nucleotide sequence