Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_717

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_715 and Pf6N2E2_716 are separated by 73 nucleotidesPf6N2E2_716 and Pf6N2E2_717 are separated by 160 nucleotidesPf6N2E2_717 and Pf6N2E2_718 are separated by 155 nucleotidesPf6N2E2_718 and Pf6N2E2_719 are separated by 88 nucleotides Pf6N2E2_715 - putative membrane protein, at 819,372 to 819,809 _715 Pf6N2E2_716 - Nitric oxide-dependent regulator DnrN or NorA, at 819,883 to 820,557 _716 Pf6N2E2_717 - Functional role page for Anaerobic nitric oxide reductase transcription regulator NorR, at 820,718 to 822,250 _717 Pf6N2E2_718 - Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5), at 822,406 to 823,053 _718 Pf6N2E2_719 - Molybdenum cofactor biosynthesis protein MoaB, at 823,142 to 823,696 _719 Position (kb) 820 821 822 823Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 819.795 kb on + strandat 819.795 kb on + strandat 819.795 kb on + strandat 819.796 kb on - strandat 819.864 kb on - strandat 819.978 kb on - strand, within Pf6N2E2_716at 820.181 kb on + strand, within Pf6N2E2_716at 820.181 kb on + strand, within Pf6N2E2_716at 820.181 kb on + strand, within Pf6N2E2_716at 820.181 kb on + strand, within Pf6N2E2_716at 820.182 kb on - strand, within Pf6N2E2_716at 820.182 kb on - strand, within Pf6N2E2_716at 820.182 kb on - strand, within Pf6N2E2_716at 820.182 kb on - strand, within Pf6N2E2_716at 820.478 kb on + strand, within Pf6N2E2_716at 820.478 kb on + strand, within Pf6N2E2_716at 820.478 kb on + strand, within Pf6N2E2_716at 820.478 kb on + strand, within Pf6N2E2_716at 820.478 kb on + strand, within Pf6N2E2_716at 820.479 kb on - strand, within Pf6N2E2_716at 820.479 kb on - strand, within Pf6N2E2_716at 820.479 kb on - strand, within Pf6N2E2_716at 820.491 kb on + strandat 820.491 kb on + strandat 820.714 kb on + strandat 820.714 kb on + strandat 820.805 kb on + strandat 820.806 kb on - strandat 820.994 kb on + strand, within Pf6N2E2_717at 821.063 kb on + strand, within Pf6N2E2_717at 821.064 kb on - strand, within Pf6N2E2_717at 821.064 kb on - strand, within Pf6N2E2_717at 821.064 kb on - strand, within Pf6N2E2_717at 821.064 kb on - strand, within Pf6N2E2_717at 821.064 kb on - strand, within Pf6N2E2_717at 821.319 kb on + strand, within Pf6N2E2_717at 821.319 kb on + strand, within Pf6N2E2_717at 821.319 kb on + strand, within Pf6N2E2_717at 821.319 kb on + strand, within Pf6N2E2_717at 821.320 kb on - strand, within Pf6N2E2_717at 821.554 kb on + strand, within Pf6N2E2_717at 821.744 kb on + strand, within Pf6N2E2_717at 821.745 kb on - strand, within Pf6N2E2_717at 821.745 kb on - strand, within Pf6N2E2_717at 821.745 kb on - strand, within Pf6N2E2_717at 822.220 kb on + strandat 822.220 kb on + strandat 822.220 kb on + strandat 822.221 kb on - strandat 822.650 kb on - strand, within Pf6N2E2_718at 822.732 kb on - strand, within Pf6N2E2_718at 822.732 kb on - strand, within Pf6N2E2_718at 822.732 kb on - strand, within Pf6N2E2_718at 822.782 kb on - strand, within Pf6N2E2_718at 823.067 kb on + strandat 823.067 kb on + strandat 823.067 kb on + strandat 823.068 kb on - strandat 823.068 kb on - strandat 823.084 kb on - strandat 823.193 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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819,795 + -0.1
819,795 + -0.3
819,795 + +1.4
819,796 - +2.4
819,864 - +0.1
819,978 - Pf6N2E2_716 0.14 -1.4
820,181 + Pf6N2E2_716 0.44 -0.3
820,181 + Pf6N2E2_716 0.44 +0.4
820,181 + Pf6N2E2_716 0.44 -0.8
820,181 + Pf6N2E2_716 0.44 +0.8
820,182 - Pf6N2E2_716 0.44 -1.3
820,182 - Pf6N2E2_716 0.44 -0.7
820,182 - Pf6N2E2_716 0.44 +0.1
820,182 - Pf6N2E2_716 0.44 +1.4
820,478 + Pf6N2E2_716 0.88 -0.6
820,478 + Pf6N2E2_716 0.88 -1.6
820,478 + Pf6N2E2_716 0.88 -1.6
820,478 + Pf6N2E2_716 0.88 +0.2
820,478 + Pf6N2E2_716 0.88 +0.6
820,479 - Pf6N2E2_716 0.88 +0.1
820,479 - Pf6N2E2_716 0.88 -1.6
820,479 - Pf6N2E2_716 0.88 -0.9
820,491 + -0.4
820,491 + -0.7
820,714 + -0.2
820,714 + +1.4
820,805 + -3.4
820,806 - +1.4
820,994 + Pf6N2E2_717 0.18 -1.2
821,063 + Pf6N2E2_717 0.23 -1.6
821,064 - Pf6N2E2_717 0.23 -0.3
821,064 - Pf6N2E2_717 0.23 -1.0
821,064 - Pf6N2E2_717 0.23 -0.9
821,064 - Pf6N2E2_717 0.23 -0.9
821,064 - Pf6N2E2_717 0.23 -2.0
821,319 + Pf6N2E2_717 0.39 -3.0
821,319 + Pf6N2E2_717 0.39 -0.6
821,319 + Pf6N2E2_717 0.39 -4.0
821,319 + Pf6N2E2_717 0.39 -1.2
821,320 - Pf6N2E2_717 0.39 -0.9
821,554 + Pf6N2E2_717 0.55 -2.8
821,744 + Pf6N2E2_717 0.67 +0.4
821,745 - Pf6N2E2_717 0.67 -2.2
821,745 - Pf6N2E2_717 0.67 -2.2
821,745 - Pf6N2E2_717 0.67 -1.0
822,220 + -1.4
822,220 + -2.7
822,220 + -1.7
822,221 - -3.0
822,650 - Pf6N2E2_718 0.38 -2.3
822,732 - Pf6N2E2_718 0.50 -3.4
822,732 - Pf6N2E2_718 0.50 -3.1
822,732 - Pf6N2E2_718 0.50 -0.7
822,782 - Pf6N2E2_718 0.58 -0.2
823,067 + +0.9
823,067 + +1.4
823,067 + -1.2
823,068 - +1.4
823,068 - +0.4
823,084 - +0.9
823,193 - -0.3

Or see this region's nucleotide sequence