Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_70 and Pf6N2E2_71 are separated by 63 nucleotides Pf6N2E2_71 and Pf6N2E2_72 are separated by 252 nucleotides
Pf6N2E2_70 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 112,610 to 113,464
_70
Pf6N2E2_71 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 113,528 to 114,160
_71
Pf6N2E2_72 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 114,413 to 115,333
_72
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 112.569 kb on + strand at 112.569 kb on + strand at 112.569 kb on + strand at 112.570 kb on - strand at 112.570 kb on - strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.572 kb on - strand at 112.572 kb on - strand at 112.572 kb on - strand at 112.572 kb on - strand at 112.589 kb on - strand at 112.589 kb on - strand at 112.603 kb on + strand at 112.620 kb on + strand at 112.620 kb on + strand at 112.621 kb on - strand at 112.682 kb on + strand at 112.683 kb on - strand at 112.991 kb on + strand, within Pf6N2E2_70 at 112.992 kb on - strand, within Pf6N2E2_70 at 112.992 kb on - strand, within Pf6N2E2_70 at 113.000 kb on + strand, within Pf6N2E2_70 at 113.000 kb on + strand, within Pf6N2E2_70 at 113.001 kb on - strand, within Pf6N2E2_70 at 113.370 kb on - strand, within Pf6N2E2_70 at 113.370 kb on - strand, within Pf6N2E2_70 at 113.376 kb on + strand, within Pf6N2E2_70 at 113.417 kb on + strand at 113.417 kb on + strand at 113.624 kb on + strand, within Pf6N2E2_71 at 113.745 kb on + strand, within Pf6N2E2_71 at 113.745 kb on + strand, within Pf6N2E2_71 at 113.825 kb on + strand, within Pf6N2E2_71 at 113.826 kb on - strand, within Pf6N2E2_71 at 113.915 kb on + strand, within Pf6N2E2_71 at 113.915 kb on + strand, within Pf6N2E2_71 at 113.915 kb on + strand, within Pf6N2E2_71 at 113.916 kb on - strand, within Pf6N2E2_71 at 113.916 kb on - strand, within Pf6N2E2_71 at 114.053 kb on + strand, within Pf6N2E2_71 at 114.053 kb on + strand, within Pf6N2E2_71 at 114.196 kb on + strand at 114.196 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 112,569 + +0.6 112,569 + -1.7 112,569 + -0.7 112,570 - -0.0 112,570 - +0.5 112,571 + -0.8 112,571 + -0.1 112,571 + +1.0 112,571 + -0.6 112,571 + -0.1 112,571 + -0.0 112,571 + -0.4 112,572 - +0.6 112,572 - -0.5 112,572 - -1.4 112,572 - -0.1 112,589 - -0.8 112,589 - -0.6 112,603 + -0.2 112,620 + +1.4 112,620 + +0.4 112,621 - +0.2 112,682 + -1.9 112,683 - +0.8 112,991 + Pf6N2E2_70 0.45 -2.9 112,992 - Pf6N2E2_70 0.45 +0.4 112,992 - Pf6N2E2_70 0.45 -2.4 113,000 + Pf6N2E2_70 0.46 -1.9 113,000 + Pf6N2E2_70 0.46 -0.7 113,001 - Pf6N2E2_70 0.46 -0.0 113,370 - Pf6N2E2_70 0.89 -0.4 113,370 - Pf6N2E2_70 0.89 -0.8 113,376 + Pf6N2E2_70 0.90 +0.4 113,417 + -0.6 113,417 + -0.0 113,624 + Pf6N2E2_71 0.15 -2.0 113,745 + Pf6N2E2_71 0.34 -2.3 113,745 + Pf6N2E2_71 0.34 -3.0 113,825 + Pf6N2E2_71 0.47 -3.3 113,826 - Pf6N2E2_71 0.47 -2.8 113,915 + Pf6N2E2_71 0.61 -3.5 113,915 + Pf6N2E2_71 0.61 -3.0 113,915 + Pf6N2E2_71 0.61 -4.5 113,916 - Pf6N2E2_71 0.61 -1.9 113,916 - Pf6N2E2_71 0.61 -2.8 114,053 + Pf6N2E2_71 0.83 -2.1 114,053 + Pf6N2E2_71 0.83 -2.2 114,196 + +1.4 114,196 + -0.9
Or see this region's nucleotide sequence