Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_705

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_702 and Pf6N2E2_703 overlap by 4 nucleotidesPf6N2E2_703 and Pf6N2E2_704 are separated by 190 nucleotidesPf6N2E2_704 and Pf6N2E2_705 are separated by 24 nucleotidesPf6N2E2_705 and Pf6N2E2_706 are separated by 198 nucleotides Pf6N2E2_702 - Heme d1 biosynthesis protein NirF, at 807,015 to 808,193 _702 Pf6N2E2_703 - Cytochrome c55X precursor NirC, at 808,190 to 808,519 _703 Pf6N2E2_704 - Cytochrome c551/c552, at 808,710 to 809,018 _704 Pf6N2E2_705 - Cytochrome cd1 nitrite reductase (EC:1.7.2.1), at 809,043 to 810,722 _705 Pf6N2E2_706 - Nitric oxide reductase activation protein NorQ, at 810,921 to 811,724 _706 Pf6N2E2_707 - Nitric oxide reductase activation protein NorE, at 811,708 to 812,292 _707 Position (kb) 809 810 811Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 808.377 kb on + strand, within Pf6N2E2_703at 808.377 kb on + strand, within Pf6N2E2_703at 808.377 kb on + strand, within Pf6N2E2_703at 808.377 kb on + strand, within Pf6N2E2_703at 808.377 kb on + strand, within Pf6N2E2_703at 808.377 kb on + strand, within Pf6N2E2_703at 808.378 kb on - strand, within Pf6N2E2_703at 808.378 kb on - strand, within Pf6N2E2_703at 808.534 kb on + strandat 808.534 kb on + strandat 808.535 kb on - strandat 808.711 kb on + strandat 808.712 kb on - strandat 808.712 kb on - strandat 808.712 kb on - strandat 808.715 kb on + strandat 808.716 kb on - strandat 808.716 kb on - strandat 808.805 kb on + strand, within Pf6N2E2_704at 808.806 kb on - strand, within Pf6N2E2_704at 808.806 kb on - strand, within Pf6N2E2_704at 808.901 kb on + strand, within Pf6N2E2_704at 808.902 kb on - strand, within Pf6N2E2_704at 809.010 kb on - strandat 809.047 kb on + strandat 809.047 kb on + strandat 809.047 kb on + strandat 809.047 kb on + strandat 809.047 kb on + strandat 809.048 kb on - strandat 809.048 kb on - strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.068 kb on + strandat 809.069 kb on - strandat 809.218 kb on + strand, within Pf6N2E2_705at 809.219 kb on - strand, within Pf6N2E2_705at 809.263 kb on + strand, within Pf6N2E2_705at 809.264 kb on - strand, within Pf6N2E2_705at 809.368 kb on + strand, within Pf6N2E2_705at 809.368 kb on + strand, within Pf6N2E2_705at 809.369 kb on - strand, within Pf6N2E2_705at 809.369 kb on - strand, within Pf6N2E2_705at 809.369 kb on - strand, within Pf6N2E2_705at 809.369 kb on - strand, within Pf6N2E2_705at 809.369 kb on - strand, within Pf6N2E2_705at 809.453 kb on - strand, within Pf6N2E2_705at 809.453 kb on - strand, within Pf6N2E2_705at 809.666 kb on - strand, within Pf6N2E2_705at 809.666 kb on - strand, within Pf6N2E2_705at 809.749 kb on + strand, within Pf6N2E2_705at 809.959 kb on + strand, within Pf6N2E2_705at 809.959 kb on + strand, within Pf6N2E2_705at 809.959 kb on + strand, within Pf6N2E2_705at 809.959 kb on + strand, within Pf6N2E2_705at 809.960 kb on - strand, within Pf6N2E2_705at 810.100 kb on + strand, within Pf6N2E2_705at 810.245 kb on + strand, within Pf6N2E2_705at 810.430 kb on + strand, within Pf6N2E2_705at 810.430 kb on + strand, within Pf6N2E2_705at 810.430 kb on + strand, within Pf6N2E2_705at 810.430 kb on + strand, within Pf6N2E2_705at 810.431 kb on - strand, within Pf6N2E2_705at 810.431 kb on - strand, within Pf6N2E2_705at 810.451 kb on + strand, within Pf6N2E2_705at 810.452 kb on - strand, within Pf6N2E2_705at 810.671 kb on + strandat 810.672 kb on - strandat 810.758 kb on + strandat 810.758 kb on + strandat 810.758 kb on + strandat 810.758 kb on + strandat 810.759 kb on - strandat 810.759 kb on - strandat 810.759 kb on - strandat 810.963 kb on + strandat 810.963 kb on + strandat 810.963 kb on + strandat 810.963 kb on + strandat 810.963 kb on + strandat 810.964 kb on - strandat 810.964 kb on - strandat 811.102 kb on - strand, within Pf6N2E2_706at 811.102 kb on - strand, within Pf6N2E2_706at 811.102 kb on - strand, within Pf6N2E2_706at 811.102 kb on - strand, within Pf6N2E2_706at 811.221 kb on - strand, within Pf6N2E2_706at 811.221 kb on - strand, within Pf6N2E2_706at 811.228 kb on - strand, within Pf6N2E2_706at 811.305 kb on + strand, within Pf6N2E2_706at 811.306 kb on - strand, within Pf6N2E2_706at 811.306 kb on - strand, within Pf6N2E2_706at 811.365 kb on + strand, within Pf6N2E2_706at 811.365 kb on + strand, within Pf6N2E2_706at 811.365 kb on + strand, within Pf6N2E2_706at 811.365 kb on + strand, within Pf6N2E2_706at 811.366 kb on - strand, within Pf6N2E2_706at 811.366 kb on - strand, within Pf6N2E2_706at 811.469 kb on + strand, within Pf6N2E2_706

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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808,377 + Pf6N2E2_703 0.57 -0.9
808,377 + Pf6N2E2_703 0.57 -1.9
808,377 + Pf6N2E2_703 0.57 -1.1
808,377 + Pf6N2E2_703 0.57 -2.1
808,377 + Pf6N2E2_703 0.57 -1.3
808,377 + Pf6N2E2_703 0.57 +0.3
808,378 - Pf6N2E2_703 0.57 -0.2
808,378 - Pf6N2E2_703 0.57 -0.0
808,534 + -0.5
808,534 + -0.2
808,535 - +0.4
808,711 + +1.4
808,712 - -1.9
808,712 - -0.1
808,712 - +0.5
808,715 + -0.4
808,716 - +0.3
808,716 - +0.4
808,805 + Pf6N2E2_704 0.31 -1.2
808,806 - Pf6N2E2_704 0.31 +1.4
808,806 - Pf6N2E2_704 0.31 -2.3
808,901 + Pf6N2E2_704 0.62 -0.2
808,902 - Pf6N2E2_704 0.62 -0.5
809,010 - -0.7
809,047 + -0.2
809,047 + -0.0
809,047 + -1.0
809,047 + -0.4
809,047 + -0.4
809,048 - -0.2
809,048 - +0.5
809,068 + -0.1
809,068 + -1.2
809,068 + +0.8
809,068 + -0.8
809,068 + -1.2
809,068 + -1.5
809,068 + -0.9
809,069 - -0.4
809,218 + Pf6N2E2_705 0.10 -0.2
809,219 - Pf6N2E2_705 0.10 +0.8
809,263 + Pf6N2E2_705 0.13 +0.0
809,264 - Pf6N2E2_705 0.13 +0.4
809,368 + Pf6N2E2_705 0.19 -0.1
809,368 + Pf6N2E2_705 0.19 -0.5
809,369 - Pf6N2E2_705 0.19 -0.4
809,369 - Pf6N2E2_705 0.19 -0.9
809,369 - Pf6N2E2_705 0.19 +1.0
809,369 - Pf6N2E2_705 0.19 -0.6
809,369 - Pf6N2E2_705 0.19 -0.3
809,453 - Pf6N2E2_705 0.24 +0.2
809,453 - Pf6N2E2_705 0.24 -1.5
809,666 - Pf6N2E2_705 0.37 -0.2
809,666 - Pf6N2E2_705 0.37 -1.4
809,749 + Pf6N2E2_705 0.42 -1.4
809,959 + Pf6N2E2_705 0.55 -0.6
809,959 + Pf6N2E2_705 0.55 -0.4
809,959 + Pf6N2E2_705 0.55 +1.4
809,959 + Pf6N2E2_705 0.55 -0.3
809,960 - Pf6N2E2_705 0.55 +1.0
810,100 + Pf6N2E2_705 0.63 -1.5
810,245 + Pf6N2E2_705 0.72 -0.6
810,430 + Pf6N2E2_705 0.83 -2.0
810,430 + Pf6N2E2_705 0.83 -0.8
810,430 + Pf6N2E2_705 0.83 -1.6
810,430 + Pf6N2E2_705 0.83 +1.2
810,431 - Pf6N2E2_705 0.83 -0.4
810,431 - Pf6N2E2_705 0.83 -0.6
810,451 + Pf6N2E2_705 0.84 -1.0
810,452 - Pf6N2E2_705 0.84 +0.4
810,671 + -1.0
810,672 - +1.7
810,758 + -0.6
810,758 + +1.4
810,758 + -1.7
810,758 + -1.1
810,759 - +0.6
810,759 - +2.2
810,759 - -0.5
810,963 + -2.1
810,963 + -0.2
810,963 + -0.7
810,963 + -2.3
810,963 + -1.6
810,964 - -0.6
810,964 - -1.7
811,102 - Pf6N2E2_706 0.23 +1.4
811,102 - Pf6N2E2_706 0.23 -1.3
811,102 - Pf6N2E2_706 0.23 -1.9
811,102 - Pf6N2E2_706 0.23 -1.6
811,221 - Pf6N2E2_706 0.37 +1.4
811,221 - Pf6N2E2_706 0.37 -2.9
811,228 - Pf6N2E2_706 0.38 -2.7
811,305 + Pf6N2E2_706 0.48 -0.2
811,306 - Pf6N2E2_706 0.48 +0.4
811,306 - Pf6N2E2_706 0.48 -2.5
811,365 + Pf6N2E2_706 0.55 -0.4
811,365 + Pf6N2E2_706 0.55 -2.0
811,365 + Pf6N2E2_706 0.55 -1.0
811,365 + Pf6N2E2_706 0.55 -3.3
811,366 - Pf6N2E2_706 0.55 -4.0
811,366 - Pf6N2E2_706 0.55 -3.0
811,469 + Pf6N2E2_706 0.68 -2.4

Or see this region's nucleotide sequence