Experiment: Nitrate reduction, replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_671 and Pf6N2E2_672 are separated by 51 nucleotides Pf6N2E2_672 and Pf6N2E2_673 overlap by 8 nucleotides Pf6N2E2_673 and Pf6N2E2_674 are separated by 12 nucleotides Pf6N2E2_674 and Pf6N2E2_675 overlap by 8 nucleotides
Pf6N2E2_671 - 2-methylaconitate racemase, at 776,215 to 777,243
_671
Pf6N2E2_672 - ABC-type polar amino acid transport system, ATPase component, at 777,295 to 778,017
_672
Pf6N2E2_673 - Probable permease of ABC transporter, at 778,010 to 778,660
_673
Pf6N2E2_674 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 778,673 to 779,338
_674
Pf6N2E2_675 - 2-Methylcitrate dehydratase AcnD, at 779,331 to 781,106
_675
Position (kb) 778
779 Strain fitness (log2 ratio) -2
-1
0
1
2 at 777.217 kb on + strand at 777.217 kb on + strand at 777.218 kb on - strand at 777.218 kb on - strand at 777.494 kb on - strand, within Pf6N2E2_672 at 777.548 kb on + strand, within Pf6N2E2_672 at 777.548 kb on + strand, within Pf6N2E2_672 at 777.549 kb on - strand, within Pf6N2E2_672 at 778.078 kb on - strand, within Pf6N2E2_673 at 778.078 kb on - strand, within Pf6N2E2_673 at 778.081 kb on - strand, within Pf6N2E2_673 at 778.282 kb on - strand, within Pf6N2E2_673 at 778.282 kb on - strand, within Pf6N2E2_673 at 778.375 kb on - strand, within Pf6N2E2_673 at 778.623 kb on + strand at 778.624 kb on - strand at 778.651 kb on + strand at 778.651 kb on + strand at 778.652 kb on - strand at 778.740 kb on + strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.741 kb on - strand, within Pf6N2E2_674 at 778.765 kb on - strand, within Pf6N2E2_674 at 778.765 kb on - strand, within Pf6N2E2_674 at 778.794 kb on + strand, within Pf6N2E2_674 at 778.795 kb on - strand, within Pf6N2E2_674 at 778.891 kb on - strand, within Pf6N2E2_674 at 778.891 kb on - strand, within Pf6N2E2_674 at 779.106 kb on + strand, within Pf6N2E2_674 at 779.107 kb on - strand, within Pf6N2E2_674 at 779.115 kb on + strand, within Pf6N2E2_674 at 779.115 kb on + strand, within Pf6N2E2_674 at 779.116 kb on - strand, within Pf6N2E2_674 at 779.116 kb on - strand, within Pf6N2E2_674 at 779.204 kb on + strand, within Pf6N2E2_674 at 779.228 kb on + strand, within Pf6N2E2_674 at 779.229 kb on - strand, within Pf6N2E2_674 at 779.328 kb on + strand at 779.329 kb on - strand at 779.329 kb on - strand at 779.471 kb on - strand at 779.471 kb on - strand at 779.480 kb on - strand at 779.480 kb on - strand at 779.480 kb on - strand at 779.606 kb on + strand, within Pf6N2E2_675 at 779.656 kb on + strand, within Pf6N2E2_675 at 779.656 kb on + strand, within Pf6N2E2_675 at 779.656 kb on + strand, within Pf6N2E2_675 at 779.656 kb on + strand, within Pf6N2E2_675 at 779.657 kb on - strand, within Pf6N2E2_675
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A remove 777,217 + +0.5 777,217 + +0.6 777,218 - -0.3 777,218 - +1.4 777,494 - Pf6N2E2_672 0.28 -0.5 777,548 + Pf6N2E2_672 0.35 +0.1 777,548 + Pf6N2E2_672 0.35 +0.6 777,549 - Pf6N2E2_672 0.35 -1.3 778,078 - Pf6N2E2_673 0.10 -0.2 778,078 - Pf6N2E2_673 0.10 -1.1 778,081 - Pf6N2E2_673 0.11 -0.2 778,282 - Pf6N2E2_673 0.42 -1.7 778,282 - Pf6N2E2_673 0.42 -1.3 778,375 - Pf6N2E2_673 0.56 +0.2 778,623 + +1.6 778,624 - +0.7 778,651 + -0.1 778,651 + +2.4 778,652 - +2.4 778,740 + Pf6N2E2_674 0.10 +0.6 778,741 - Pf6N2E2_674 0.10 -2.0 778,741 - Pf6N2E2_674 0.10 +0.8 778,741 - Pf6N2E2_674 0.10 +0.1 778,741 - Pf6N2E2_674 0.10 +0.2 778,765 - Pf6N2E2_674 0.14 +0.0 778,765 - Pf6N2E2_674 0.14 +0.4 778,794 + Pf6N2E2_674 0.18 +0.0 778,795 - Pf6N2E2_674 0.18 -1.0 778,891 - Pf6N2E2_674 0.33 -0.5 778,891 - Pf6N2E2_674 0.33 -0.3 779,106 + Pf6N2E2_674 0.65 +0.1 779,107 - Pf6N2E2_674 0.65 -1.2 779,115 + Pf6N2E2_674 0.66 +0.7 779,115 + Pf6N2E2_674 0.66 -0.6 779,116 - Pf6N2E2_674 0.67 -0.4 779,116 - Pf6N2E2_674 0.67 +1.4 779,204 + Pf6N2E2_674 0.80 +0.1 779,228 + Pf6N2E2_674 0.83 -0.9 779,229 - Pf6N2E2_674 0.83 -0.0 779,328 + +2.4 779,329 - +1.4 779,329 - -0.6 779,471 - +0.1 779,471 - -0.6 779,480 - -0.2 779,480 - +0.6 779,480 - -0.7 779,606 + Pf6N2E2_675 0.15 +0.4 779,656 + Pf6N2E2_675 0.18 +1.1 779,656 + Pf6N2E2_675 0.18 +0.4 779,656 + Pf6N2E2_675 0.18 -0.4 779,656 + Pf6N2E2_675 0.18 +1.4 779,657 - Pf6N2E2_675 0.18 +0.9
Or see this region's nucleotide sequence