Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_610

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_609 and Pf6N2E2_610 are separated by 193 nucleotidesPf6N2E2_610 and Pf6N2E2_611 are separated by 219 nucleotides Pf6N2E2_609 - L-arabonate dehydratase (EC 4.2.1.25) (from data), at 691,355 to 693,091 _609 Pf6N2E2_610 - Nitrate/nitrite transporter, at 693,285 to 694,607 _610 Pf6N2E2_611 - 2-dehydro-3-deoxy-L-arabinonate dehydratase (EC 4.2.1.43) (from data), at 694,827 to 695,819 _611 Position (kb) 693 694 695Strain fitness (log2 ratio) -2 -1 0 1at 692.386 kb on + strand, within Pf6N2E2_609at 692.386 kb on + strand, within Pf6N2E2_609at 692.387 kb on - strand, within Pf6N2E2_609at 692.400 kb on + strand, within Pf6N2E2_609at 693.306 kb on - strandat 693.317 kb on + strandat 693.468 kb on + strand, within Pf6N2E2_610at 693.468 kb on + strand, within Pf6N2E2_610at 693.468 kb on + strand, within Pf6N2E2_610at 693.468 kb on + strand, within Pf6N2E2_610at 693.469 kb on - strand, within Pf6N2E2_610at 693.580 kb on + strand, within Pf6N2E2_610at 693.581 kb on - strand, within Pf6N2E2_610at 693.593 kb on + strand, within Pf6N2E2_610at 693.600 kb on + strand, within Pf6N2E2_610at 693.600 kb on + strand, within Pf6N2E2_610at 693.601 kb on - strand, within Pf6N2E2_610at 693.601 kb on - strand, within Pf6N2E2_610at 693.601 kb on - strand, within Pf6N2E2_610at 693.601 kb on - strand, within Pf6N2E2_610at 693.657 kb on + strand, within Pf6N2E2_610at 693.657 kb on + strand, within Pf6N2E2_610at 693.657 kb on + strand, within Pf6N2E2_610at 693.658 kb on - strand, within Pf6N2E2_610at 693.660 kb on + strand, within Pf6N2E2_610at 693.660 kb on + strand, within Pf6N2E2_610at 693.660 kb on + strand, within Pf6N2E2_610at 693.660 kb on + strand, within Pf6N2E2_610at 693.660 kb on + strand, within Pf6N2E2_610at 693.661 kb on - strand, within Pf6N2E2_610at 693.728 kb on + strand, within Pf6N2E2_610at 693.728 kb on + strand, within Pf6N2E2_610at 693.728 kb on + strand, within Pf6N2E2_610at 693.729 kb on - strand, within Pf6N2E2_610at 693.729 kb on - strand, within Pf6N2E2_610at 693.865 kb on - strand, within Pf6N2E2_610at 694.027 kb on - strand, within Pf6N2E2_610at 694.134 kb on + strand, within Pf6N2E2_610at 694.134 kb on + strand, within Pf6N2E2_610at 694.134 kb on + strand, within Pf6N2E2_610at 694.135 kb on - strand, within Pf6N2E2_610at 694.218 kb on + strand, within Pf6N2E2_610at 694.218 kb on + strand, within Pf6N2E2_610at 694.218 kb on + strand, within Pf6N2E2_610at 694.219 kb on - strand, within Pf6N2E2_610at 694.219 kb on - strand, within Pf6N2E2_610at 694.222 kb on + strand, within Pf6N2E2_610at 694.222 kb on + strand, within Pf6N2E2_610at 694.222 kb on + strand, within Pf6N2E2_610at 694.222 kb on + strand, within Pf6N2E2_610at 694.222 kb on + strand, within Pf6N2E2_610at 694.223 kb on - strand, within Pf6N2E2_610at 694.416 kb on + strand, within Pf6N2E2_610at 694.452 kb on + strand, within Pf6N2E2_610at 694.479 kb on + strandat 694.480 kb on - strandat 694.601 kb on + strandat 694.796 kb on + strandat 694.796 kb on + strandat 694.797 kb on - strandat 694.834 kb on + strandat 694.835 kb on - strandat 694.835 kb on - strandat 695.007 kb on + strand, within Pf6N2E2_611at 695.245 kb on - strand, within Pf6N2E2_611at 695.343 kb on + strand, within Pf6N2E2_611at 695.343 kb on + strandat 695.343 kb on + strand, within Pf6N2E2_611at 695.343 kb on + strand, within Pf6N2E2_611at 695.344 kb on - strand, within Pf6N2E2_611at 695.344 kb on - strand, within Pf6N2E2_611at 695.430 kb on + strand, within Pf6N2E2_611at 695.430 kb on + strand, within Pf6N2E2_611at 695.431 kb on - strand, within Pf6N2E2_611at 695.431 kb on - strand, within Pf6N2E2_611at 695.431 kb on - strand, within Pf6N2E2_611at 695.436 kb on + strand, within Pf6N2E2_611

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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692,386 + Pf6N2E2_609 0.59 -1.0
692,386 + Pf6N2E2_609 0.59 -1.1
692,387 - Pf6N2E2_609 0.59 -2.6
692,400 + Pf6N2E2_609 0.60 +0.1
693,306 - -0.4
693,317 + +0.4
693,468 + Pf6N2E2_610 0.14 -0.7
693,468 + Pf6N2E2_610 0.14 -0.4
693,468 + Pf6N2E2_610 0.14 +1.4
693,468 + Pf6N2E2_610 0.14 +0.2
693,469 - Pf6N2E2_610 0.14 -0.2
693,580 + Pf6N2E2_610 0.22 +0.6
693,581 - Pf6N2E2_610 0.22 -1.0
693,593 + Pf6N2E2_610 0.23 -0.3
693,600 + Pf6N2E2_610 0.24 -0.5
693,600 + Pf6N2E2_610 0.24 +0.6
693,601 - Pf6N2E2_610 0.24 +1.4
693,601 - Pf6N2E2_610 0.24 -1.8
693,601 - Pf6N2E2_610 0.24 -1.0
693,601 - Pf6N2E2_610 0.24 +1.4
693,657 + Pf6N2E2_610 0.28 +0.6
693,657 + Pf6N2E2_610 0.28 -0.4
693,657 + Pf6N2E2_610 0.28 -0.0
693,658 - Pf6N2E2_610 0.28 +0.3
693,660 + Pf6N2E2_610 0.28 +0.2
693,660 + Pf6N2E2_610 0.28 -0.3
693,660 + Pf6N2E2_610 0.28 +1.4
693,660 + Pf6N2E2_610 0.28 -0.8
693,660 + Pf6N2E2_610 0.28 -0.7
693,661 - Pf6N2E2_610 0.28 +1.1
693,728 + Pf6N2E2_610 0.33 +0.1
693,728 + Pf6N2E2_610 0.33 -2.3
693,728 + Pf6N2E2_610 0.33 -0.3
693,729 - Pf6N2E2_610 0.34 -1.5
693,729 - Pf6N2E2_610 0.34 +1.4
693,865 - Pf6N2E2_610 0.44 -0.0
694,027 - Pf6N2E2_610 0.56 -0.4
694,134 + Pf6N2E2_610 0.64 -0.3
694,134 + Pf6N2E2_610 0.64 -0.2
694,134 + Pf6N2E2_610 0.64 +0.9
694,135 - Pf6N2E2_610 0.64 -0.5
694,218 + Pf6N2E2_610 0.71 -0.6
694,218 + Pf6N2E2_610 0.71 -0.4
694,218 + Pf6N2E2_610 0.71 +0.3
694,219 - Pf6N2E2_610 0.71 -0.9
694,219 - Pf6N2E2_610 0.71 -0.6
694,222 + Pf6N2E2_610 0.71 -0.8
694,222 + Pf6N2E2_610 0.71 +1.4
694,222 + Pf6N2E2_610 0.71 -1.0
694,222 + Pf6N2E2_610 0.71 +0.1
694,222 + Pf6N2E2_610 0.71 -0.5
694,223 - Pf6N2E2_610 0.71 -0.6
694,416 + Pf6N2E2_610 0.85 -1.3
694,452 + Pf6N2E2_610 0.88 -0.7
694,479 + -0.4
694,480 - +0.4
694,601 + +1.2
694,796 + -0.5
694,796 + +0.0
694,797 - +0.3
694,834 + +0.2
694,835 - +1.2
694,835 - -0.2
695,007 + Pf6N2E2_611 0.18 -0.3
695,245 - Pf6N2E2_611 0.42 -1.5
695,343 + Pf6N2E2_611 0.52 +0.4
695,343 + -0.2
695,343 + Pf6N2E2_611 0.52 +0.1
695,343 + Pf6N2E2_611 0.52 +0.1
695,344 - Pf6N2E2_611 0.52 -0.2
695,344 - Pf6N2E2_611 0.52 -0.5
695,430 + Pf6N2E2_611 0.61 -0.5
695,430 + Pf6N2E2_611 0.61 -1.1
695,431 - Pf6N2E2_611 0.61 +0.4
695,431 - Pf6N2E2_611 0.61 +1.2
695,431 - Pf6N2E2_611 0.61 -0.5
695,436 + Pf6N2E2_611 0.61 -0.0

Or see this region's nucleotide sequence